RDB14218 - pcDNA5-FLAG/HA/SBP

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pcDNA5-FLAG/HA/SBP

Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells.

Catalog number	RDB14218
Resource name	pcDNA5-FLAG/HA/SBP
Clone info.	Expression vector of N-terminally FLAG-HA-streptavidin binding peptide (SBP) tagged proteins in mammalian cells.
Comment	The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone	pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone	5.1 kb
Selectable markers	Amp^r (E. coli), Hygro^r (mammalian cell).
Depositor\|Developer	Ui-Tei, Kumiko \|

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External Database

Reference sequence

Sequence (full)	RDB14218zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14218	pcDNA5-FLAG/HA/SBP	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14218_A6Fup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,tet ope,tet ope,insert(FLAG,HA,SBP),bGH pA Sequence file: RDB14218_A6Fuc.seq
>D03279A6_A6Fu_1_CMV-Forward_H02_23.ab1 1 NNNNNNNNNN NNNNNTNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA 61 GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG 121 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA 181 GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TCCACCGCGG TGGCGGCCGC 241 CACCATGGAT TACAAGGATG ACGACGACGA TAAGTACCCA TACGATGTTC CAGATTACGC 301 TTCTATGGAC GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG 361 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC GGGNGCCCTG 421 CCCGGGCGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT 481 CGAGGGGGGG CCCGGTACCT TAATTAATTA AGGTACCAGG TAAGTGTACC CAATTCGCCC 541 TATAGTGAGT CGTATTACAA TTCACTCGAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT 601 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAANGTGCC 661 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GANTANGTGT 721 CATTCTATTC TGGGGGGTGG GGTGGGGCAN GACAGCAAGG GGGANGNNTN NNAAGANAAT 781 AGCANGCATG CTGGGGATGC GGNGGGCTCT ATNGCTTCTG ANGCNGAAAN AACCAGCNGG 841 GGGCTNTANG GGGTATCCCC NCNCGCCCNN NANCGGNNCN NTAANCNCNG NGGNNNNNNN 901 NNN //
Primer: BGHpA_F Region: bGH pA,FRT,HygR Sequence file: RDB14218_A6Fud.seq
>D03279A6_A6Fu_1_BGHpA_F_H03_24.ab1 1 NNNNNNNNNN NNNNNTTCNG GGNGGNGGGG NGGGGCANGN NAGCAAGGGG GNNGNNTGGG 61 AAGANAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA 421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC 481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC 541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG 601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC 661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG 721 CACAGGGTGT CACGTTGCAA GANCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG 781 TCGCGGANGN CATGGATGCG ATCGCTGCGG CCGATCTTAN CCAGACGANC GGGTTNNGCC 841 CNTTCNGANC GCAAGGANNC GGNCNATACA CTACATGGNG NGNNTTCATA NNCGCNANTG 901 NNNNNNCCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2022.08.12

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