Sequencing and PCR primers for primer information.
Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells.
| Catalog number | RDB14218 |
|---|---|
| Resource name | pcDNA5-FLAG/HA/SBP |
| Clone info. | Expression vector of N-terminally FLAG-HA-streptavidin binding peptide (SBP) tagged proteins in mammalian cells. The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting. |
| Vector backbone | pcDNA 5/FRT/TO (Plasmid) |
| Size of vector backbone | 5.1 kb |
| Selectable markers | Amp^r (E. coli), Hygro^r (mammalian cell). |
| Depositor | Ui-Tei, Kumiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB14218 | pcDNA5-FLAG/HA/SBP | DNA solution | Please contact us. |
Electronic file
Featured content
References
| original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
|---|
User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14218_A6Fup1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB14218_A6Fua.seq |
|---|---|
|
>D03279A6_A6Fu_1_CMV-Forward_H02_23.ab1 1 ATTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG 121 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA 181 GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TCCACCGCGG TGGCGGCCGC 241 CACCATGGAT TACAAGGATG ACGACGACGA TAAGTACCCA TACGATGTTC CAGATTACGC 301 TTCTATGGAC GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG 361 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC GGGAGCCCTG 421 CCCGGGCGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT 481 CGAGGGGGGG CCCGGTACCT TAATTAATTA AGGTACCAGG TAAGTGTACC CAATTCGCCC 541 TATAGTGAGT CGTATTACAA TTCACTCGAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT 601 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC 661 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT 721 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT 781 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG 841 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 901 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CCTTTTCGCT 961 TTCTTCCCTT CCTTTCTCGC CACGTTCGGC GGCCTTTCCC CGTTCAGGCT CTAAATCCGG 1021 GGCCTCCATT AAGGATTCCG GAATTTTAAG TGCCT // |
|
| Primer: BGHpA_F | RDB14218_A6Fub.seq |
|
>D03279A6_A6Fu_1_BGHpA_F_H03_24.ab1 1 CGTAAGGTTA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 61 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA 421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC 481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC 541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG 601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC 661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG 721 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG 781 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC 841 CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG 901 CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG 961 CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCCGAGGACT GCCCCCGAAG TCTGGCACCT 1021 CGTGCACGCG AATTCGCTCG ACCATGTCCT GACGGACATT GACCGCATAA CAGTCGGGAT 1081 CCAATTGAAC TG // |
|
Please visit Sequencing and PCR primers for primer information.
References
| original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
|---|
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl