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pcDNA5-FLAG/HA/SBP

Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells.

Catalog number RDB14218
Resource name pcDNA5-FLAG/HA/SBP
Clone info. Expression vector of N-terminally FLAG-HA-streptavidin binding peptide (SBP) tagged proteins in mammalian cells.
The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Depositor Ui-Tei, Kumiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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RDB14218 pcDNA5-FLAG/HA/SBP DNA solution Please contact us.

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References and tips

Electronic file

Featured content

References

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14218_A6Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14218_A6Fua.seq
>D03279A6_A6Fu_1_CMV-Forward_H02_23.ab1
    1 ATTCACTCCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG
  121 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
  181 GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TCCACCGCGG TGGCGGCCGC
  241 CACCATGGAT TACAAGGATG ACGACGACGA TAAGTACCCA TACGATGTTC CAGATTACGC
  301 TTCTATGGAC GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
  361 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC GGGAGCCCTG
  421 CCCGGGCGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT
  481 CGAGGGGGGG CCCGGTACCT TAATTAATTA AGGTACCAGG TAAGTGTACC CAATTCGCCC
  541 TATAGTGAGT CGTATTACAA TTCACTCGAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT
  601 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
  661 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT
  721 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT
  781 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG
  841 GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT
  901 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CCTTTTCGCT
  961 TTCTTCCCTT CCTTTCTCGC CACGTTCGGC GGCCTTTCCC CGTTCAGGCT CTAAATCCGG
1021 GGCCTCCATT AAGGATTCCG GAATTTTAAG TGCCT
//
Primer: BGHpA_F RDB14218_A6Fub.seq
>D03279A6_A6Fu_1_BGHpA_F_H03_24.ab1
    1 CGTAAGGTTA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG
   61 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
  121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
  181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
  241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
  301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
  361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA
  421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
  481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC
  541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG
  601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC
  661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG
  721 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
  781 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC
  841 CATTCGGACC GCAAGGAATC GGTCAATACA CTACATGGCG TGATTTCATA TGCGCGATTG
  901 CTGATCCCCA TGTGTATCAC TGGCAAACTG TGATGGACGA CACCGTCAGT GCGTCCGTCG
  961 CGCAGGCTCT CGATGAGCTG ATGCTTTGGG CCCGAGGACT GCCCCCGAAG TCTGGCACCT
 1021 CGTGCACGCG AATTCGCTCG ACCATGTCCT GACGGACATT GACCGCATAA CAGTCGGGAT
1081 CCAATTGAAC TG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2018.12.10

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