Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA5-FLAG/HA/SBP (#RDB14218)

Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells.

Clone info. Expression vector of N-terminally FLAG-HA-streptavidin binding peptide (SBP) tagged proteins in mammalian cells.
Comment The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Depositor|Developer Ui-Tei, Kumiko |
 
Sequence (full) RDB14218zzt01.seq compiled by us

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。

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RDB14218 pcDNA5-FLAG/HA/SBP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA5-FLAG/HA/SBP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14218).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14218_A6Fup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,tet ope,tet ope,insert(FLAG,HA,SBP),bGH pA
Sequence file: RDB14218_A6Fuc.seq check
>D03279A6_A6Fu_1_CMV-Forward_H02_23.ab1
    1 NNNNNNNNNN NNNNNTNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA
   61 GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG
  121 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
  181 GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TCCACCGCGG TGGCGGCCGC
  241 CACCATGGAT TACAAGGATG ACGACGACGA TAAGTACCCA TACGATGTTC CAGATTACGC
  301 TTCTATGGAC GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
  361 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC GGGNGCCCTG
  421 CCCGGGCGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT
  481 CGAGGGGGGG CCCGGTACCT TAATTAATTA AGGTACCAGG TAAGTGTACC CAATTCGCCC
  541 TATAGTGAGT CGTATTACAA TTCACTCGAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT
  601 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAANGTGCC
  661 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GANTANGTGT
  721 CATTCTATTC TGGGGGGTGG GGTGGGGCAN GACAGCAAGG GGGANGNNTN NNAAGANAAT
  781 AGCANGCATG CTGGGGATGC GGNGGGCTCT ATNGCTTCTG ANGCNGAAAN AACCAGCNGG
  841 GGGCTNTANG GGGTATCCCC NCNCGCCCNN NANCGGNNCN NTAANCNCNG NGGNNNNNNN
901 NNN
//
Primer: BGHpA_F (Pr0373)
Region: bGH pA,FRT,HygR
Sequence file: RDB14218_A6Fud.seq check
>D03279A6_A6Fu_1_BGHpA_F_H03_24.ab1
    1 NNNNNNNNNN NNNNNTTCNG GGNGGNGGGG NGGGGCANGN NAGCAAGGGG GNNGNNTGGG
   61 AAGANAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
  121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
  181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
  241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
  301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
  361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA
  421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
  481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC
  541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG
  601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC
  661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG
  721 CACAGGGTGT CACGTTGCAA GANCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
  781 TCGCGGANGN CATGGATGCG ATCGCTGCGG CCGATCTTAN CCAGACGANC GGGTTNNGCC
  841 CNTTCNGANC GCAAGGANNC GGNCNATACA CTACATGGNG NGNNTTCATA NNCGCNANTG
901 NNNNNNCCNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]