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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA5-FLAG/HA/SBP

Expression vector of N-terminally FLAG/HA/SBP tagged proteins in mammalian cells.

Catalog number RDB14218
Resource name pcDNA5-FLAG/HA/SBP
Clone info. Expression vector of N-terminally FLAG-HA-streptavidin binding peptide (SBP) tagged proteins in mammalian cells.
Comment The peptide sequence of FLAG-tag is DYKDDDDDK instead of canonical sequence (DYKDDDDK). Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA 5/FRT/TO (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hygro^r (mammalian cell).
Depositor|Developer Ui-Tei, Kumiko |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB14218zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB14218 pcDNA5-FLAG/HA/SBP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14218_A6Fup1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,tet ope,tet ope,insert(FLAG,HA,SBP),bGH pA
            Sequence file: RDB14218_A6Fuc.seq check
            >D03279A6_A6Fu_1_CMV-Forward_H02_23.ab1
                1 NNNNNNNNNN NNNNNTNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGNN GTCTATATAA
               61 GCAGAGCTCT CCCTATCAGT GATAGAGATC TCCCTATCAG TGATAGAGAT CGTCGACGAG
              121 CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT GACCTCCATA
              181 GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGCGTT TCCACCGCGG TGGCGGCCGC
              241 CACCATGGAT TACAAGGATG ACGACGACGA TAAGTACCCA TACGATGTTC CAGATTACGC
              301 TTCTATGGAC GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
              361 CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC GGGNGCCCTG
              421 CCCGGGCGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT
              481 CGAGGGGGGG CCCGGTACCT TAATTAATTA AGGTACCAGG TAAGTGTACC CAATTCGCCC
              541 TATAGTGAGT CGTATTACAA TTCACTCGAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT
              601 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAANGTGCC
              661 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GANTANGTGT
              721 CATTCTATTC TGGGGGGTGG GGTGGGGCAN GACAGCAAGG GGGANGNNTN NNAAGANAAT
              781 AGCANGCATG CTGGGGATGC GGNGGGCTCT ATNGCTTCTG ANGCNGAAAN AACCAGCNGG
              841 GGGCTNTANG GGGTATCCCC NCNCGCCCNN NANCGGNNCN NTAANCNCNG NGGNNNNNNN
            901 NNN
            //
            Primer: BGHpA_F
            Region: bGH pA,FRT,HygR
            Sequence file: RDB14218_A6Fud.seq check
            >D03279A6_A6Fu_1_BGHpA_F_H03_24.ab1
                1 NNNNNNNNNN NNNNNTTCNG GGNGGNGGGG NGGGGCANGN NAGCAAGGGG GNNGNNTGGG
               61 AAGANAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA
              121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
              181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
              241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
              301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
              361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA
              421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC
              481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC
              541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG
              601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC
              661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG
              721 CACAGGGTGT CACGTTGCAA GANCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG
              781 TCGCGGANGN CATGGATGCG ATCGCTGCGG CCGATCTTAN CCAGACGANC GGGTTNNGCC
              841 CNTTCNGANC GCAAGGANNC GGNCNATACA CTACATGGNG NGNNTTCATA NNCGCNANTG
            901 NNNNNNCCNN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            2022.08.12

            GNP_filter3_RDBDEP_html_220812.pl