RDB14214 - pcDNA3.1-myc-GFP-TNRC6A

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pcDNA3.1-myc-GFP-TNRC6A (#RDB14214)

Expression vector of myc-GFP-tagged human TNRC6A.

Clone info.	Expression vector of myc-GFP-tagged human TNRC6A.
Comment	Expression was confirmed by the depositor with microscopic observation and Western blotting.
Vector backbone	pcDNA3.1-myc (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA
Depositor\|Developer	Ui-Tei, Kumiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14214	pcDNA3.1-myc-GFP-TNRC6A	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-myc-GFP-TNRC6A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14214).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14214_A6Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14214_A6Fua.seq
>D03279A2_A6Fu_1_CMV-Forward_A07_01.ab1 1 ACGTAATAAC CATCCGCCCC ATTGAACGCA AAATGGGGCG GTAGGCGGTG TACGGTGGGA 61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG 121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GCCCACCATG GAACAAAAAC 181 TCATCTCAGA AGAGGATCTG TCCGGCGGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG 241 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT 301 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA 361 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT 421 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG 481 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG 541 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT 601 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG 661 TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAAGG TGAACTTCAA AGATCCGCCA 721 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG 781 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA 841 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAA 901 TCACTTCTCG GCAATGGACG AAGCTGTACA AGGTCCCGGG CGGCAAAGCT TTAATGGGAT 961 GCCTGGATTC CTGCCTTCCC AGTTCTGGAA ATCAGAAGAA GAAACAATCG ACTATCATGG 1021 GCTTTCAAGG AAACCACAGG GTGGGTGGAA AAAAAAGATG GGCCCCTTTC GGGAAATAGC 1081 AACTGGGAAA CCTTTGGGGT TCCCCCAAAA ACCAAGCTTT GGGTAGCGTT GGGGATAACC 1141 CAGCCAGAGC CCATAATAAA TA //
Primer: TNRC6A_GW1 Sequence file: RDB14214_A6Fub.seq
>D03279A2_A6Fu_1_TNRC6A_GW1_A04_01.ab1 1 AAAAAATGCG GTGCTATGGT TCTATTACTC TGGAGACAAA TGTTCAGGCC CTAATGGCCA 61 AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT GGCGTAAATG GTCCTATGGG 121 CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG TGGGAATCTG GTGCAGCAAA 181 CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT TCTGGAGGAA GTCGAAGAGG 241 ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC AGCGTTGAGT GGAACAAACT 301 GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT GGTAAGACGT TTACAAATGG 361 ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA TCTCAGACAA ATGAGCAAAG 421 CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT GGTAGTACAG AAAGCACTGG 481 ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA GACAGAAGAA AAATTGATCA 541 GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA GATCCACGTG TCCTGTCCAA 601 CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC TGGGATACAG AAACATCACC 661 TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG GGAAGCTCTG CAACACAGAC 721 TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT GGTAATGATA CCTCATCTGT 781 ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA GATTCCAAAG GCTCAAACTG 841 CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG GTCAAGAGCA ATCAGTGGGG 901 GGAAATTGCA AGAGGAGAAA GCTGCATGGA AATGACTCGC AAAAGAATAA ACAGGGATGG 961 GGGTGATGGG ACAAAAATCA AGCCAAAGGT GGTCCTGTTT CTGCCAGTGA TACTGGGGAG 1021 AACCTTCAGG ATATCATGGG TGAAGGCCCA ATAAGAAAAT CCAGCCCTTC AAG //
Primer: TNRC6A_GW2 Sequence file: RDB14214_A6Fuc.seq
>D03279A2_A6Fu_1_TNRC6A_GW2_H04_22.ab1 1 CCACAACCAC GGCCAGGGGG GGGGTGGGAA GATGATTCTG CTGCTACAGG AATGGTCAAG 61 AGCAATCAGT GGGGGAATTG CAAAGAGGAG AAGGCTGCAT GGAATGACTC GCAAAAGAAT 121 AAACAGGGAT GGGGTGATGG ACAAAAATCA AGCCAAGGGT GGTCTGTTTC TGCCAGTGAT 181 AACTGGGGAG AAACTTCAAG GAATAACCAT TGGGGTGAGG CCAATAAGAA ATCCAGCTCA 241 GGAGGTAGTG ACAGTGACAG GTCCGTTTCC GGTTGGAACG AACTTGGTAA AACTAGTTCT 301 TTCACTTGGG GAAACAACAT AAATCCAAAT AATTCATCAG GATGGGATGA ATCTTCTAAA 361 CCTACTCCTT CCCAGGGATG GGGAGACCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA 421 GATTCGTCAA AGCCAGTCAG CTCTCCAGAC TGGAACAAGC AACAAGACAT TGTTGGATCT 481 TGGGGAATCC CACCAGCTAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG GGGACCTATA 541 CCAGCCCCAG CAAAAGAAGA AGAACCCACA GGCTGGGAGG AACCATCCCC AGAATCTATA 601 CGTCGCAAAA TGGAGATTGA TGATGGAACT TCAGCTTGGG GAGATCCAAG CAAATACAAC 661 TACAAAAATG TGAACATGTG GAACAAAAAC GTCCCAAATG GCAACAGCCG TTCAGACCAG 721 CAAAGCACAG GTACATCAGC TGCTAACGCC TGGCAAGTGC CATCTCAAAC AAAGAGGCAA 781 GCAGTGGCTC TGGCTGGGGG TGAGCCCTGG GGGGGAGCCT TTCTACTCCA GCCACAACTG 841 TGGATAATGG TACTTCAGCA TGGGGGTAAG CCCATAGACA GTGGGTCCCA GCTGGGGGGG 901 AACCCATTGC TGCGGCATCC AGCACATCCA CGTGGGGCTC CAGCTCTGTT GGGTCCACAA 961 GCATTAAGCA AATCTGGGCC AAAATCTATG CAAGATGGCT GGGTGTGGGT GGATGATATG 1021 CCAATTGCCT GGGAAATCGC CCCACTGGCT GGGAAGAGGA AAGAGGATGT GGAGATTGGA 1081 ATGTGGAATA GTTAATTCCA TCTCAAGAAG CTTGACTTCA TTCTTTAATG GCCACCATAT 1141 CCAAAGGAAA ATGTCATCGA AGGTCTTTGA ATGGCAAAAG GAAAAAGGAC ATAGGGGTTG 1201 A //
Primer: TNRC6A_NES-NLS-GW3 Sequence file: RDB14214_A6Fud.seq
>D03279A2_A6Fu_1_TNRC6A_NES-NLS-GW3_D07_10.ab1 1 TGGGCTGGCT GATATATTGA TGGGGAGCTT CATGCTTTGA CTGGACATAA ACTGCATGTT 61 TTGGGATTCA TCTGCTACAA TGCCATCCTT ATCAAAATAA GGATTGCGCT CCATGGAAGA 121 CTCTTTGGAA ATCTGAGGCC GAGAACCAGG GCCTTTCCCG ACCGTTCGAT TGTAATCACC 181 AATATTTAGG CTATGTTTAT CTATCTCCAT TCGTTTGTCT TGTAACATTC CTCCAGAAAG 241 GTCCATCTTA TTGCTTTGTA CATTTTCCTC TGGTGAGTCT CTCGAAAAAC TGATGTTTGA 301 AAACTGTTTA ACAAATGGAT TTATCCACGC TTCTTCTTGT TTGTTTCCAC CTTTCATCAT 361 TCCCCTTTCC CTTCTCCTTT TTTTGCCACT CAGACCCTTC GATGACATTT TCTTTGTATA 421 TGGTGGCCAA TTTAAAGATG AGTTAAGCTC TTGAGATGAA TTACTATTCC ACATTCCAAT 481 CTCCACATCC TCTTCCTCTT CCCAGCCAGT GGGGCGATTT CCAGGCAATG GCATATCATC 541 ACCACACCAG CCATCTTGCA TAGATTTTGG CCCAGATTTG CTTAATGCTT GTGGACCAAC 601 AGAGCTGGAG CCCCACGTGG ATGTGCTGGA TGCCGCAGCA ATGGGTTCCC CCCAGCTGGG 661 ACCACTGTCT ATGGGCTTAC CCCATGCTGA AGTACCATTA TCCACAGTTG TGGCTGGAGT 721 AGAAGGCTCC CCCCAGGGCT CACCCCAGCC AGAGCCACTG CTTGCCTCTT TGTTTGAGAT 781 GGCACTTGCA GGCGTTAGCA GCTGATGTAC CTGTGCTTGC TGGTCTGAAC GGCTGTTGCC 841 ATTTGGGACG TTTTTTGTTC CACATGTTCA CATTTTTTGT AGTTGTATTT GCTTTGGATC 901 TCCCCAAGCT GAAGTTCCAT CATCAATCTT CCATTTTTGC GACGTATAGA TTCTGGGGGA 961 TGGTTCCTTC CCAGCCCTGT GGGTTTCTTC TTTCTTTTTG CTGGGGGCTG GGTATAAGGT 1021 TCCCCCCCAG CCCAGCCTGT ACCCAAGGAG GTTTTGGCCC TGTAGCCTTG ATGGGGATTT 1081 CGTCAAGAAT CCCACATTGT CTGATGCCTT GATTCAGTTC TTTGGAGA //
Primer: EGFP-C Sequence file: RDB14214_A6Fue.seq
>D03279A2_A6Fu_1_EGFP-C_B01_04.ab1 1 CAGGCATCTC GGGCATGGAC GAGCTGTACA GTCCGGCGGC AAGCTTATGG ATGCTGATTC 61 TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG GCTTCAGGGA ACACAGGTGG 121 TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC CAAAACAAGT TTGTAGTTGG 181 TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG CCATGGGGTT TTTCCCATGG 241 AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT GAAAGCAAAT CTGAAAGTAG 301 CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA TCAAATGGAG GGTTAAATCC 361 AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA GTATTAGAGA ACAATGGACT 421 TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT AATATTCAGT GCAGTACTAT 481 AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG AGTGGTGGTT CTACCCATGG 541 TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA AGTGGTACAC AGAAGGTTTC 601 ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT GGACCAAATA ACACTACTAA 661 CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG AATAATGAGC TGCCTAGTAG 721 TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT CAGATGCAGC TCCATCAGTA 781 TGAATGGCAC TTCCCTTTCT CACCTTAGCA ATGGGAGAGT CAAAAAGTGG GAGGCTCTTA 841 TGGTACTACA TGGGGTGCCC TATGGGTTCT AATTTACTCT GGGAGACAAA TGTTCAGCCC 901 TAAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT ATGCAGCTGT GTAAATGGTC 961 CTATGGGCAC TACCTTTCCA GGTAACACAA ACAAAGGAGT GGTGTGTGGA TCTGTCCAGC 1021 ACTTCCAGAG TAACTCATGG ATGATGCGCA ATCTGA //
Primer: BGH_rev2 Sequence file: RDB14214_A6Fuf.seq
>D03279A2_A6Fu_1_BGH_rev2_C04_07.ab1 1 CTATCGGATA GGAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CATGGACTCT CCACCCCCAC 121 CCAGGTGGTC AACAGAAAGA AAAGCGTTAA TGGGAGATGG GCTGCTAATT CCTCGGGGGT 181 CGCTGCTGCT TGGGGGACCC CACAGGCTTG TGGAATAATG CGGGGTCCCC CAGAGTGAAG 241 TGCCGTGAAG GTCTCCACAG TTAGTTCCCG ACAGCCCAGC ACCATTCCAG TGATTGAGAT 301 CGGTCCGGCT GGAGAATGAG TGGGAACAGT CGAGGGAGCC CAGCCGGCTC TGGCTGGACC 361 CGAGAGACTG CCAGCCGGGA GAAGGGGTCA GAGACTGGCT TTGTGCAAAG AAACGACTGA 421 TCTCCTCTTC ACTGGCAAAC TCAGCAAGAA TAGTAGTGTT CCCCAGTACA CACATGTGCA 481 GAGACTTTTG TGCCTTCACT ACCTCTTCTT TTGAACTGTA GCGGACCAGA GCATTTCCGT 541 GAGGGAGGTT CAGGTGGAAT GTGATCAGCG GGCCGTGCTG CATGCACAGA GTGCGCAGAG 601 TTGAGCCATC GATCTGAGGT GTAAGGTTTT TTAGAACAAG CCAATTTGTT ATTCTCCCTG 661 AGCTGCTCTC ACCCCAGCTG GAACCTGGGG TATATCTGGC GTCAGAATTT CCCCATCCTC 721 CACCTATACG AAGGGGAGAA TTATCCCACG TAGACAAGGG TGGCTTCTGA CCAGTCAGTC 781 CCGGAGGTGG GCGGGACGGA GCAGTGATGT TTTTTAGGTG GCAAAGGGAC CTTCCACAGC 841 TCATGAGCCA GAGAGGTGTT TGTAACTGAA CCAGGAGACC ATGTCAATTT GGAATCACTA 901 TTTCTGGCTG AAGTGCTTTG TGCTGTACTG CTGAGGGGAA CGTTGTAGTT TGGAGCACGA 961 ATGGATGACC AGGGCACTAG TTGAAGCAGC GTATGTTTCA CGATGAAGAA TGACCCACTG 1021 TTTCTGGTCC ATGAAGTTGA TTCAAACCTT GCCGCACAGT ATAAGTGCCA AAGGACGGAT 1081 TTTAGTGAAC CACTG //
Primer: SV40pro_F Sequence file: RDB14214_A6Fug.seq
>D03279A2_A6Fu_1_SV40pro_F_D04_10.ab1 1 CCTGGTCTGG GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT 121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC 661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 901 CTTCTTGACG AGTTCTTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCC GACCAAGCGA 961 CGCCCAACCC TGCCAATCAC GAGATTTCGA TTTCACCCGC CCGGCGTTCT ATGAAAGGAT 1021 GGGCTTCGGA TCGATTTTCA GGACGTCGGC TGGATGAATC CATCCAGTCG GCGGGGGGAA 1081 TCGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]