Resource data sheet
Prev.

pcDNA3.1-myc-GFP-TNRC6A

Expression vector of myc-GFP-tagged human TNRC6A.

Catalog number RDB14214
Resource name pcDNA3.1-myc-GFP-TNRC6A
Clone info. Expression vector of myc-GFP-tagged human TNRC6A.
Expression was confirmed by the depositor with microscopic observation and Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name human TNRC6A cDNA Aquorea victoria green flurescent protein (GFP) cDNA
Depositor Ui-Tei, Kumiko |
 
Other clones in our bank human TNRC6A (NCBI Gene 27327) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14214 pcDNA3.1-myc-GFP-TNRC6A DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14214_A6Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14214_A6Fua.seq
>D03279A2_A6Fu_1_CMV-Forward_A07_01.ab1
    1 ACGTAATAAC CATCCGCCCC ATTGAACGCA AAATGGGGCG GTAGGCGGTG TACGGTGGGA
   61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
  121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGGCTA GCCCACCATG GAACAAAAAC
  181 TCATCTCAGA AGAGGATCTG TCCGGCGGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
  241 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
  301 CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA
  361 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT
  421 GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
  481 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG
  541 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
  601 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
  661 TCTATATCAT GGCCGACAAG CAGAAGAACG GCATCAAAGG TGAACTTCAA AGATCCGCCA
  721 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
  781 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA
  841 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAA
  901 TCACTTCTCG GCAATGGACG AAGCTGTACA AGGTCCCGGG CGGCAAAGCT TTAATGGGAT
  961 GCCTGGATTC CTGCCTTCCC AGTTCTGGAA ATCAGAAGAA GAAACAATCG ACTATCATGG
 1021 GCTTTCAAGG AAACCACAGG GTGGGTGGAA AAAAAAGATG GGCCCCTTTC GGGAAATAGC
 1081 AACTGGGAAA CCTTTGGGGT TCCCCCAAAA ACCAAGCTTT GGGTAGCGTT GGGGATAACC
1141 CAGCCAGAGC CCATAATAAA TA
//
Primer: TNRC6A_GW1 RDB14214_A6Fub.seq
>D03279A2_A6Fu_1_TNRC6A_GW1_A04_01.ab1
    1 AAAAAATGCG GTGCTATGGT TCTATTACTC TGGAGACAAA TGTTCAGGCC CTAATGGCCA
   61 AGCTAATGGT GACACTGTGA ATGCAACTCT AATGCAGCCT GGCGTAAATG GTCCTATGGG
  121 CACTAACTTT CAAGTTAACA CAAACAAAGG AGGTGGTGTG TGGGAATCTG GTGCAGCAAA
  181 CTCCCAGAGT ACATCATGGG GAAGTGGAAA TGGCGCAAAT TCTGGAGGAA GTCGAAGAGG
  241 ATGGGGAACC CCTGCACAAA ACACTGGCAC TAATTTACCC AGCGTTGAGT GGAACAAACT
  301 GCCTAGCAAT CAGCATTCCA ATGATAGTGC AAATGGCAAT GGTAAGACGT TTACAAATGG
  361 ATGGAAATCT ACTGAGGAAG AGGATCAGGG TTCTGCCACA TCTCAGACAA ATGAGCAAAG
  421 CAGTGTGTGG GCCAAAACAG GAGGTACAGT GGAGAGCGAT GGTAGTACAG AAAGCACTGG
  481 ACGCCTTGAG GAAAAAGGAA CTGGGGAAAG TCAGAGTAGA GACAGAAGAA AAATTGATCA
  541 GCACACATTA CTCCAAAGCA TTGTAAACAG AACTGACTTA GATCCACGTG TCCTGTCCAA
  601 CTCTGGTTGG GGACAGACTC CTATTAAGCA GAATACTGCC TGGGATACAG AAACATCACC
  661 TAGAGGGGAA CGAAAGACTG ACAATGGGAC AGAGGCCTGG GGAAGCTCTG CAACACAGAC
  721 TTTTAACTCA GGGGCATGTA TAGATAAGAC TAGCCCTAAT GGTAATGATA CCTCATCTGT
  781 ATCAGGGTGG GGCGATCCCA AACCTGCTCT GAGGTGGGGA GATTCCAAAG GCTCAAACTG
  841 CCAGGGGGGG TGGGAAGATG ATTCTGCTGC TACAGGAATG GTCAAGAGCA ATCAGTGGGG
  901 GGAAATTGCA AGAGGAGAAA GCTGCATGGA AATGACTCGC AAAAGAATAA ACAGGGATGG
  961 GGGTGATGGG ACAAAAATCA AGCCAAAGGT GGTCCTGTTT CTGCCAGTGA TACTGGGGAG
1021 AACCTTCAGG ATATCATGGG TGAAGGCCCA ATAAGAAAAT CCAGCCCTTC AAG
//
Primer: TNRC6A_GW2 RDB14214_A6Fuc.seq
>D03279A2_A6Fu_1_TNRC6A_GW2_H04_22.ab1
    1 CCACAACCAC GGCCAGGGGG GGGGTGGGAA GATGATTCTG CTGCTACAGG AATGGTCAAG
   61 AGCAATCAGT GGGGGAATTG CAAAGAGGAG AAGGCTGCAT GGAATGACTC GCAAAAGAAT
  121 AAACAGGGAT GGGGTGATGG ACAAAAATCA AGCCAAGGGT GGTCTGTTTC TGCCAGTGAT
  181 AACTGGGGAG AAACTTCAAG GAATAACCAT TGGGGTGAGG CCAATAAGAA ATCCAGCTCA
  241 GGAGGTAGTG ACAGTGACAG GTCCGTTTCC GGTTGGAACG AACTTGGTAA AACTAGTTCT
  301 TTCACTTGGG GAAACAACAT AAATCCAAAT AATTCATCAG GATGGGATGA ATCTTCTAAA
  361 CCTACTCCTT CCCAGGGATG GGGAGACCCT CCAAAGTCTA ATCAGTCTCT AGGTTGGGGA
  421 GATTCGTCAA AGCCAGTCAG CTCTCCAGAC TGGAACAAGC AACAAGACAT TGTTGGATCT
  481 TGGGGAATCC CACCAGCTAC AGGCAAACCT CCTGGTACAG GCTGGCTGGG GGGACCTATA
  541 CCAGCCCCAG CAAAAGAAGA AGAACCCACA GGCTGGGAGG AACCATCCCC AGAATCTATA
  601 CGTCGCAAAA TGGAGATTGA TGATGGAACT TCAGCTTGGG GAGATCCAAG CAAATACAAC
  661 TACAAAAATG TGAACATGTG GAACAAAAAC GTCCCAAATG GCAACAGCCG TTCAGACCAG
  721 CAAAGCACAG GTACATCAGC TGCTAACGCC TGGCAAGTGC CATCTCAAAC AAAGAGGCAA
  781 GCAGTGGCTC TGGCTGGGGG TGAGCCCTGG GGGGGAGCCT TTCTACTCCA GCCACAACTG
  841 TGGATAATGG TACTTCAGCA TGGGGGTAAG CCCATAGACA GTGGGTCCCA GCTGGGGGGG
  901 AACCCATTGC TGCGGCATCC AGCACATCCA CGTGGGGCTC CAGCTCTGTT GGGTCCACAA
  961 GCATTAAGCA AATCTGGGCC AAAATCTATG CAAGATGGCT GGGTGTGGGT GGATGATATG
 1021 CCAATTGCCT GGGAAATCGC CCCACTGGCT GGGAAGAGGA AAGAGGATGT GGAGATTGGA
 1081 ATGTGGAATA GTTAATTCCA TCTCAAGAAG CTTGACTTCA TTCTTTAATG GCCACCATAT
 1141 CCAAAGGAAA ATGTCATCGA AGGTCTTTGA ATGGCAAAAG GAAAAAGGAC ATAGGGGTTG
1201 A
//
Primer: TNRC6A_NES-NLS-GW3 RDB14214_A6Fud.seq
>D03279A2_A6Fu_1_TNRC6A_NES-NLS-GW3_D07_10.ab1
    1 TGGGCTGGCT GATATATTGA TGGGGAGCTT CATGCTTTGA CTGGACATAA ACTGCATGTT
   61 TTGGGATTCA TCTGCTACAA TGCCATCCTT ATCAAAATAA GGATTGCGCT CCATGGAAGA
  121 CTCTTTGGAA ATCTGAGGCC GAGAACCAGG GCCTTTCCCG ACCGTTCGAT TGTAATCACC
  181 AATATTTAGG CTATGTTTAT CTATCTCCAT TCGTTTGTCT TGTAACATTC CTCCAGAAAG
  241 GTCCATCTTA TTGCTTTGTA CATTTTCCTC TGGTGAGTCT CTCGAAAAAC TGATGTTTGA
  301 AAACTGTTTA ACAAATGGAT TTATCCACGC TTCTTCTTGT TTGTTTCCAC CTTTCATCAT
  361 TCCCCTTTCC CTTCTCCTTT TTTTGCCACT CAGACCCTTC GATGACATTT TCTTTGTATA
  421 TGGTGGCCAA TTTAAAGATG AGTTAAGCTC TTGAGATGAA TTACTATTCC ACATTCCAAT
  481 CTCCACATCC TCTTCCTCTT CCCAGCCAGT GGGGCGATTT CCAGGCAATG GCATATCATC
  541 ACCACACCAG CCATCTTGCA TAGATTTTGG CCCAGATTTG CTTAATGCTT GTGGACCAAC
  601 AGAGCTGGAG CCCCACGTGG ATGTGCTGGA TGCCGCAGCA ATGGGTTCCC CCCAGCTGGG
  661 ACCACTGTCT ATGGGCTTAC CCCATGCTGA AGTACCATTA TCCACAGTTG TGGCTGGAGT
  721 AGAAGGCTCC CCCCAGGGCT CACCCCAGCC AGAGCCACTG CTTGCCTCTT TGTTTGAGAT
  781 GGCACTTGCA GGCGTTAGCA GCTGATGTAC CTGTGCTTGC TGGTCTGAAC GGCTGTTGCC
  841 ATTTGGGACG TTTTTTGTTC CACATGTTCA CATTTTTTGT AGTTGTATTT GCTTTGGATC
  901 TCCCCAAGCT GAAGTTCCAT CATCAATCTT CCATTTTTGC GACGTATAGA TTCTGGGGGA
  961 TGGTTCCTTC CCAGCCCTGT GGGTTTCTTC TTTCTTTTTG CTGGGGGCTG GGTATAAGGT
 1021 TCCCCCCCAG CCCAGCCTGT ACCCAAGGAG GTTTTGGCCC TGTAGCCTTG ATGGGGATTT
1081 CGTCAAGAAT CCCACATTGT CTGATGCCTT GATTCAGTTC TTTGGAGA
//
Primer: EGFP-C RDB14214_A6Fue.seq
>D03279A2_A6Fu_1_EGFP-C_B01_04.ab1
    1 CAGGCATCTC GGGCATGGAC GAGCTGTACA GTCCGGCGGC AAGCTTATGG ATGCTGATTC
   61 TGCCTCCAGT TCTGAATCAG AGAGAAACAT CACTATCATG GCTTCAGGGA ACACAGGTGG
  121 TGAAAAAGAT GGCCTTCGGA ATAGCACTGG ACTTGGTTCC CAAAACAAGT TTGTAGTTGG
  181 TAGCAGCAGC AATAATGTGG GCCATGGAAG TAGTACTGGG CCATGGGGTT TTTCCCATGG
  241 AGCCATAATA AGCACATGTC AGGTCTCTGT GGATGCTCCT GAAAGCAAAT CTGAAAGTAG
  301 CAACAATAGA ATGAATGCTT GGGGCACTGT AAGTTCTTCA TCAAATGGAG GGTTAAATCC
  361 AAGCACTTTG AATTCAGCTA GCAACCATGG TGCCTGGCCA GTATTAGAGA ACAATGGACT
  421 TGCCCTAAAA GGGCCTGTAG GGAGTGGTAG TTCTGGCATT AATATTCAGT GCAGTACTAT
  481 AGGCCAGATG CCTAACAATC AGAGTATTAA CTCTAAAGTG AGTGGTGGTT CTACCCATGG
  541 TACCTGGGGA AGCCTTCAGG AAACTTGTGA ATCTGAAGTA AGTGGTACAC AGAAGGTTTC
  601 ATTCAGTGGT CAACCTCAAA ATATTACCAC TGAAATGACT GGACCAAATA ACACTACTAA
  661 CTTTATGACC TCTAGTTTAC CAAACTCCGG TTCAGTGCAG AATAATGAGC TGCCTAGTAG
  721 TAACACAGGG GCCTGGCGTG TGAGCACAAT GAATCATCCT CAGATGCAGC TCCATCAGTA
  781 TGAATGGCAC TTCCCTTTCT CACCTTAGCA ATGGGAGAGT CAAAAAGTGG GAGGCTCTTA
  841 TGGTACTACA TGGGGTGCCC TATGGGTTCT AATTTACTCT GGGAGACAAA TGTTCAGCCC
  901 TAAATGGCCA AGCTAATGGT GACACTGTGA ATGCAACTCT ATGCAGCTGT GTAAATGGTC
  961 CTATGGGCAC TACCTTTCCA GGTAACACAA ACAAAGGAGT GGTGTGTGGA TCTGTCCAGC
1021 ACTTCCAGAG TAACTCATGG ATGATGCGCA ATCTGA
//
Primer: BGH_rev2 RDB14214_A6Fuf.seq
>D03279A2_A6Fu_1_BGH_rev2_C04_07.ab1
    1 CTATCGGATA GGAGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTCTAGATTA CATGGACTCT CCACCCCCAC
  121 CCAGGTGGTC AACAGAAAGA AAAGCGTTAA TGGGAGATGG GCTGCTAATT CCTCGGGGGT
  181 CGCTGCTGCT TGGGGGACCC CACAGGCTTG TGGAATAATG CGGGGTCCCC CAGAGTGAAG
  241 TGCCGTGAAG GTCTCCACAG TTAGTTCCCG ACAGCCCAGC ACCATTCCAG TGATTGAGAT
  301 CGGTCCGGCT GGAGAATGAG TGGGAACAGT CGAGGGAGCC CAGCCGGCTC TGGCTGGACC
  361 CGAGAGACTG CCAGCCGGGA GAAGGGGTCA GAGACTGGCT TTGTGCAAAG AAACGACTGA
  421 TCTCCTCTTC ACTGGCAAAC TCAGCAAGAA TAGTAGTGTT CCCCAGTACA CACATGTGCA
  481 GAGACTTTTG TGCCTTCACT ACCTCTTCTT TTGAACTGTA GCGGACCAGA GCATTTCCGT
  541 GAGGGAGGTT CAGGTGGAAT GTGATCAGCG GGCCGTGCTG CATGCACAGA GTGCGCAGAG
  601 TTGAGCCATC GATCTGAGGT GTAAGGTTTT TTAGAACAAG CCAATTTGTT ATTCTCCCTG
  661 AGCTGCTCTC ACCCCAGCTG GAACCTGGGG TATATCTGGC GTCAGAATTT CCCCATCCTC
  721 CACCTATACG AAGGGGAGAA TTATCCCACG TAGACAAGGG TGGCTTCTGA CCAGTCAGTC
  781 CCGGAGGTGG GCGGGACGGA GCAGTGATGT TTTTTAGGTG GCAAAGGGAC CTTCCACAGC
  841 TCATGAGCCA GAGAGGTGTT TGTAACTGAA CCAGGAGACC ATGTCAATTT GGAATCACTA
  901 TTTCTGGCTG AAGTGCTTTG TGCTGTACTG CTGAGGGGAA CGTTGTAGTT TGGAGCACGA
  961 ATGGATGACC AGGGCACTAG TTGAAGCAGC GTATGTTTCA CGATGAAGAA TGACCCACTG
 1021 TTTCTGGTCC ATGAAGTTGA TTCAAACCTT GCCGCACAGT ATAAGTGCCA AAGGACGGAT
1081 TTTAGTGAAC CACTG
//
Primer: SV40pro_F RDB14214_A6Fug.seq
>D03279A2_A6Fu_1_SV40pro_F_D04_10.ab1
    1 CCTGGTCTGG GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 CTTCTTGACG AGTTCTTTCT GAGCGGGACT CTGGGGGTTC GAAATGACCC GACCAAGCGA
  961 CGCCCAACCC TGCCAATCAC GAGATTTCGA TTTCACCCGC CCGGCGTTCT ATGAAAGGAT
 1021 GGGCTTCGGA TCGATTTTCA GGACGTCGGC TGGATGAATC CATCCAGTCG GCGGGGGGAA
1081 TCGTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl