RDB14213 - pcDNA3.1-myc-GFP

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pcDNA3.1-myc-GFP

Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.

Catalog number	RDB14213
Resource name	pcDNA3.1-myc-GFP
Clone info.	Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
Comment	Expression was confirmed by the depositor with Western blotting.
Vector backbone	pcDNA3.1-myc (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name	Aquorea victoria green flurescent protein (GFP) cDNA
Depositor\|Developer	Ui-Tei, Kumiko \|

Other clones in our bank

External Database
Aquorea victoria green flurescent protein (GFP)

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.

Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19 (1): 17-35, 2013)。教育研究目的での使用に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14213	pcDNA3.1-myc-GFP	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-myc-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14213).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content	Empty Backbone (English text)
Featured content	Empty Backbone (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14213_A6Fup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward () Sequence file: RDB14213_A6Fua.seq
>D03279A1_A6Fu_1_CMV-Forward_H07_22.ab1 1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC CCACCATGGA ACAAAAACTC ATCTCAGAAG 181 AGGATCTGTC CGGCGGCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG 301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT 421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC 481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT 541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG 601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG 661 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG 721 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC 781 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA 841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG 901 ACGAGCTGTA CAAGTAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC CTCGACTGTG 961 CCCTTCTAGT TTGCCCAGCC CATCTGTTGT TTGCCCCTCC CCCCGTGGCC GTTCGATGAC 1021 CCTGCCAAGT TGCACTTCCC ACTGTCCTTT CCTATTAAAT TGAGGAAAAC TGGCCATCGT 1081 CAATTTGTCT G //
Primer: BGH_rev2 () Sequence file: RDB14213_A6Fub.seq
>D03279A1_A6Fu_1_BGH_rev2_H08_23.ab1 1 CAACGCTAGC AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTACT TGTACAGCTC GTCCATGCCG 121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG 181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG 241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG 301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA 361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC 421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC 481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG 541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG 601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG 661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG 721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC 781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCCGC CGGACAGATC CTCTTCTGAG 841 ATGAGTTTTT TGTTCCATGG TGGGCTAGCC AGCTTGGGTC TCCCTATAGT GAGTCGTATT 901 AAATTTCGAT AAGCCAGTAA GCAGTGGGTT TCTCTAGTTT AGCCAGAGAG CTCTGCTTTA 961 TATAGACCCT CCCACCCGTA CACGCCCTAC CCGCCCATTT GCGTCATGGG GGGCGGACTG 1021 GTAACGACAT TTTGGGAAAG TCCGATGATT TTGGTTGCCG AAGCGAAACT CCCATTGACG 1081 TCCATGAGGC TGGTAGACCT TG //
Primer: SV40pro_F () Sequence file: RDB14213_A6Fuc.seq
>D03279A1_A6Fu_1_SV40pro_F_H09_24.ab1 1 CCTGTCTTGC ATCAGAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 61 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC 121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG 661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 901 TTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC CAAGCGACGC 961 CCAAACCTGC CATCACGAGA TTTCGATTCA CGCGGCTTTC TATGAAGTGG GCCTTCGAAT 1021 CGTTTTCAGG ACGGCGCCTG AATGATCTCA GCGCGGGAAT CTCATGCTGG GGAGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

2023.05.22

GNP_filter3_RDBDEP_html_230512.pl