Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-myc-GFP (#RDB14213)

Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.

Clone info. Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Growth conditions LB+Amp, 37oC
Gene/insert name Aquorea victoria green flurescent protein (GFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19 (1): 17-35, 2013)。教育研究目的での使用に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14213 pcDNA3.1-myc-GFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-myc-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14213).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14213_A6Fup1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB14213_A6Fua.seq check
>D03279A1_A6Fu_1_CMV-Forward_H07_22.ab1
    1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC CCACCATGGA ACAAAAACTC ATCTCAGAAG
  181 AGGATCTGTC CGGCGGCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
  241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
  301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
  361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
  421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
  481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
  541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
  601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG
  661 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
  721 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
  781 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
  841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
  901 ACGAGCTGTA CAAGTAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC CTCGACTGTG
  961 CCCTTCTAGT TTGCCCAGCC CATCTGTTGT TTGCCCCTCC CCCCGTGGCC GTTCGATGAC
 1021 CCTGCCAAGT TGCACTTCCC ACTGTCCTTT CCTATTAAAT TGAGGAAAAC TGGCCATCGT
1081 CAATTTGTCT G
//
Primer: BGH_rev2 ()
Sequence file: RDB14213_A6Fub.seq check
>D03279A1_A6Fu_1_BGH_rev2_H08_23.ab1
    1 CAACGCTAGC AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
   61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTACT TGTACAGCTC GTCCATGCCG
  121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
  241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG
  301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA
  361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
  421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
  481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG
  541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
  601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
  661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG
  721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
  781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCCGC CGGACAGATC CTCTTCTGAG
  841 ATGAGTTTTT TGTTCCATGG TGGGCTAGCC AGCTTGGGTC TCCCTATAGT GAGTCGTATT
  901 AAATTTCGAT AAGCCAGTAA GCAGTGGGTT TCTCTAGTTT AGCCAGAGAG CTCTGCTTTA
  961 TATAGACCCT CCCACCCGTA CACGCCCTAC CCGCCCATTT GCGTCATGGG GGGCGGACTG
 1021 GTAACGACAT TTTGGGAAAG TCCGATGATT TTGGTTGCCG AAGCGAAACT CCCATTGACG
1081 TCCATGAGGC TGGTAGACCT TG
//
Primer: SV40pro_F ()
Sequence file: RDB14213_A6Fuc.seq check
>D03279A1_A6Fu_1_SV40pro_F_H09_24.ab1
    1 CCTGTCTTGC ATCAGAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC
   61 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
  661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
  781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
  841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
  901 TTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC CAAGCGACGC
  961 CCAAACCTGC CATCACGAGA TTTCGATTCA CGCGGCTTTC TATGAAGTGG GCCTTCGAAT
1021 CGTTTTCAGG ACGGCGCCTG AATGATCTCA GCGCGGGAAT CTCATGCTGG GGAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [link to RRC of NBRP]