Sequencing and PCR primers for primer information.
Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
| Catalog number | RDB14213 |
|---|---|
| Resource name | pcDNA3.1-myc-GFP |
| Clone info. | Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells. Expression was confirmed by the depositor with Western blotting. |
| Vector backbone | pcDNA3.1-myc (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell). |
| Gene/insert name | Aquorea victoria green flurescent protein (GFP) cDNA |
| Depositor | Ui-Tei, Kumiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only. |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB14213 | pcDNA3.1-myc-GFP | DNA solution |
Electronic file
Original reference
| original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
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Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
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| Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14213_A6Fup1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | RDB14213_A6Fua.seq |
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>D03279A1_A6Fu_1_CMV-Forward_H07_22.ab1 1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC CCACCATGGA ACAAAAACTC ATCTCAGAAG 181 AGGATCTGTC CGGCGGCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA 241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG 301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC 361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT 421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC 481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT 541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG 601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG 661 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG 721 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC 781 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA 841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG 901 ACGAGCTGTA CAAGTAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC CTCGACTGTG 961 CCCTTCTAGT TTGCCCAGCC CATCTGTTGT TTGCCCCTCC CCCCGTGGCC GTTCGATGAC 1021 CCTGCCAAGT TGCACTTCCC ACTGTCCTTT CCTATTAAAT TGAGGAAAAC TGGCCATCGT 1081 CAATTTGTCT G // |
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| Primer: BGH_rev2 | RDB14213_A6Fub.seq |
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>D03279A1_A6Fu_1_BGH_rev2_H08_23.ab1 1 CAACGCTAGC AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTACT TGTACAGCTC GTCCATGCCG 121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG 181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG 241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG 301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA 361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC 421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC 481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG 541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG 601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG 661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG 721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC 781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCCGC CGGACAGATC CTCTTCTGAG 841 ATGAGTTTTT TGTTCCATGG TGGGCTAGCC AGCTTGGGTC TCCCTATAGT GAGTCGTATT 901 AAATTTCGAT AAGCCAGTAA GCAGTGGGTT TCTCTAGTTT AGCCAGAGAG CTCTGCTTTA 961 TATAGACCCT CCCACCCGTA CACGCCCTAC CCGCCCATTT GCGTCATGGG GGGCGGACTG 1021 GTAACGACAT TTTGGGAAAG TCCGATGATT TTGGTTGCCG AAGCGAAACT CCCATTGACG 1081 TCCATGAGGC TGGTAGACCT TG // |
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| Primer: SV40pro_F | RDB14213_A6Fuc.seq |
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>D03279A1_A6Fu_1_SV40pro_F_H09_24.ab1 1 CCTGTCTTGC ATCAGAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 61 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC 121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG 661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 901 TTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC CAAGCGACGC 961 CCAAACCTGC CATCACGAGA TTTCGATTCA CGCGGCTTTC TATGAAGTGG GCCTTCGAAT 1021 CGTTTTCAGG ACGGCGCCTG AATGATCTCA GCGCGGGAAT CTCATGCTGG GGAGT // |
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Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. |
|---|
2019.05.16
GNP_filter3_RDBDEP_html_190430.pl