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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-myc-GFP

Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.

Catalog number RDB14213
Resource name pcDNA3.1-myc-GFP
Clone info. Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Gene/insert name Aquorea victoria green flurescent protein (GFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |
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External Database
Aquorea victoria green flurescent protein (GFP)

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19 (1): 17-35, 2013)。教育研究目的での使用に限る。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB14213 pcDNA3.1-myc-GFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14213_A6Fup1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB14213_A6Fua.seq check
            >D03279A1_A6Fu_1_CMV-Forward_H07_22.ab1
                1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
               61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
              121 CTCACTATAG GGAGACCCAA GCTGGCTAGC CCACCATGGA ACAAAAACTC ATCTCAGAAG
              181 AGGATCTGTC CGGCGGCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
              241 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
              301 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
              361 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
              421 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
              481 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
              541 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
              601 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG
              661 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
              721 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
              781 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
              841 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
              901 ACGAGCTGTA CAAGTAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC CTCGACTGTG
              961 CCCTTCTAGT TTGCCCAGCC CATCTGTTGT TTGCCCCTCC CCCCGTGGCC GTTCGATGAC
             1021 CCTGCCAAGT TGCACTTCCC ACTGTCCTTT CCTATTAAAT TGAGGAAAAC TGGCCATCGT
            1081 CAATTTGTCT G
            //
            Primer: BGH_rev2
            Sequence file: RDB14213_A6Fub.seq check
            >D03279A1_A6Fu_1_BGH_rev2_H08_23.ab1
                1 CAACGCTAGC AGCACGGGGG AGGGGCAACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
               61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGACTACT TGTACAGCTC GTCCATGCCG
              121 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
              181 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
              241 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG
              301 ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA
              361 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
              421 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
              481 TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG
              541 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
              601 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
              661 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG TGGCATCGCC CTCGCCCTCG
              721 CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
              781 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACCATGCCGC CGGACAGATC CTCTTCTGAG
              841 ATGAGTTTTT TGTTCCATGG TGGGCTAGCC AGCTTGGGTC TCCCTATAGT GAGTCGTATT
              901 AAATTTCGAT AAGCCAGTAA GCAGTGGGTT TCTCTAGTTT AGCCAGAGAG CTCTGCTTTA
              961 TATAGACCCT CCCACCCGTA CACGCCCTAC CCGCCCATTT GCGTCATGGG GGGCGGACTG
             1021 GTAACGACAT TTTGGGAAAG TCCGATGATT TTGGTTGCCG AAGCGAAACT CCCATTGACG
            1081 TCCATGAGGC TGGTAGACCT TG
            //
            Primer: SV40pro_F
            Sequence file: RDB14213_A6Fuc.seq check
            >D03279A1_A6Fu_1_SV40pro_F_H09_24.ab1
                1 CCTGTCTTGC ATCAGAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC
               61 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC
              121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
              181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
              241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
              301 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
              361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
              421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
              481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
              541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
              601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG
              661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
              721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
              781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
              841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
              901 TTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTTCGAA ATGACCGACC CAAGCGACGC
              961 CCAAACCTGC CATCACGAGA TTTCGATTCA CGCGGCTTTC TATGAAGTGG GCCTTCGAAT
            1021 CGTTTTCAGG ACGGCGCCTG AATGATCTCA GCGCGGGAAT CTCATGCTGG GGAGT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874.

            2022.07.13

            GNP_filter3_RDBDEP_html_220713.pl