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pcDNA3.1-myc-GFP (#RDB14213)

Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nishi, K., RNA 19 (1): 17-35 (2013). PMID 23150874. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of N-terminally myc-GFP-tagged proteins in mammalian cells.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pcDNA3.1-myc (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell).
Growth conditions LB+Amp, 37oC
Gene/insert name Aquorea victoria green flurescent protein (GFP) cDNA
Depositor|Developer Ui-Tei, Kumiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19 (1): 17-35, 2013)。教育研究目的での使用に限る。

Catalog # Resource name Shipping form Fee
RDB14213 pcDNA3.1-myc-GFP DNA solution

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-myc-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14213).

Reference section:

Nishi, K., Nishi, A., Nagasawa, T., Ui-Tei, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nishi, K., Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus. RNA 19 (1): 17-35 (2013). PMID 23150874. [PubMed] [Article] [RRC of NBRP]