Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGimRET

Expression vector of GimRET (Glycine inserted mutant FRET probe) for mammalian cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14204_A6Fop1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mTurquoise2-M_F (Pr0577) Region: ECFP 3',insert 5'(YFP1G 5') Sequence file: RDB14204_A6Fod.seq
>14204_14204_B1GgF_mTurquoise2-M_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNCTCGCCGN NNNCTACCNG CAGAACACCC CCATCGGCGA CGGCCCCGTG    61 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG   121 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG   181 GACGAGCTGT ACAAGTCCGG AGGCAGCACC AGCGGTACTG GAGGTTCAGG CGGCTCAGGT   241 GGAACTGGTG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG   301 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC   361 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG   421 CCCACCCTCG TGACCACCTT CGGCTACGGC CTGCAGTGCT TCGCCCGCTA CCCCGACCAC   481 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC   541 ATCTTCTTCA AGGACGACGG CAACTACNAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC   601 ACCCTGGTGN ACCGCATCGA GCTGNAGGGC ATCGANTTCA AGGAGGANGG CNACATNCNT   661 GGGGCACAAG CTGGANTACN NCGGNNANAN AGCCNCNACG TCNATATCAN NNNNNANNNG 721 CAGNNNNNNN GNNTCNNNNN NNANNNNNNN NNN //
Primer: pAxEF R2 (Pr0059) Region: SV40 pA,insert 3'(YFP1G) Sequence file: RDB14204_A6Foe.seq
>14204_14204_B1GgF_pAxEF_R2_F03_18_ABI24.ab1     1 NNNNNNNNNN NNTTNNNTTG TGNANNTTGT GANGCTATTG CTTTATTTGT AACCATTATA    61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG   121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT   181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACTG CAGAATTCGA   241 AGCTTGAGCT CGAGTTACTT GTACAGCTCG TCCATGCCGA GAGTGATCCC GGCGGCGGTC   301 ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG GGTCTTTGCT CAGGGCGGAC   361 TGGTAGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC CGTCGCCGAT GGGGGTGTTC   421 TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA TGTTGTGGCG GATCTTGAAG   481 TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT ANACNNTGTG GCTGTTGTAT   541 CCGTTGTACT CCNGCTTGNN CCCCANGATG TTGNNGTCCT CCTTGAANNN NNNNNNCCTN   601 NNNCTCGNNG CGGNNNNNNN NNGNNGNCNC CNNNNAANNT NNNNNNCNNC GCGGGNNNTN 661 GNANNTGCCG NCNNCNNTGN ANNANNANNN NGNNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,ECFP 5' Sequence file: RDB14204_A6Fof.seq
>14204_14204_B1GgF_CMV_Forward_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNN GCNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGTCGCCA CCATGGTGAG   121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT   181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT   241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC   301 CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA   361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA   421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG   481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA   541 GTACAACTAC ATCAGCCACA ACGTCTATAT CACCGCCGAC NAGCAGAAGA ACGGCATCAA   601 GGCCAACTTC AAGATCCGCC ACAACATCGA GGANGGCAGC GTGCAGCTCG CCGANCACTA 661 CCNGCANAAN ACCCCCNTCN GCGANNGNCC CNNNCTGCTG NCNNANNNNN NNNNN //
Primer: FP-N_F1 (Pr0920) Region: ECFP 3',insert 5'(YFP1G 5') Sequence file: RDB14204_A6Fog.seq
>14204_14204_B1GgF_EP-N_F1_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNGTCCGN CCATNNCCCG AAGGCTACGT CCNGGAGCGC ACCATCTTCT    61 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG   121 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA   181 AGCTGGAGTA CAACTACATC AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG   241 GCATCAAGGC CAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG   301 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT   361 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC   421 TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGTCCG   481 GAGGCAGCAC CAGCGGTACT GGAGGTTCAG GCGGCTCAGG TGGAACTGGT GTGAGCAAGG   541 GCGANGANCT GTTCACCGGG GTGGTGCCCN TCCTGNTCGA GCTGGACGGC GACGTAANCG   601 GNCNCAAGTT CNGCGTGTCC GGCGANGGCN ANGNNGANGN CNNCNANNNN NNGCTGANCC 661 TNNNGTTCNT CTGNNNNNNN GGNNNNCTGN CCNNNNCNNN NNCNNNCCNN NNNNNNNN //
Primer: pGL3_RV5F (Pr0227) Region: insert 3'(YFP1G 3') Sequence file: RDB14204_A6Foh.seq
>D03467A2_A6Fo_1_pGL3_RV5F_A03_03.ab1     1 NNNNNNNNNN NNNNNNNNGG CTGNNTANGN NNNGTTATCT AGATCCGGTG GNTCCCGGGC    61 CCGCGGTACC GTCGACTGCA GAATTCGAAG CTTGAGCTCG AGTTACTTGT ACAGCTCGTC   121 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT   181 CTCGTTGGGG TCTTTGCTCA GGGCGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG   241 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC   301 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC   361 CATGATATAG ACGTTGTGGC TGTTGTATCC GTTGTACTCC AGCTTGTGCC CCAGGATGTT   421 GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC   481 GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC   541 CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG   601 GTAGCGGGCG AAGCACTGCA GGCCGTAGCC GAAGGTGGTC ACGAGGGTGG GCCAGGGCAC   661 GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTANG TGGCATCGCC   721 CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG   781 GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTNCTC ACACCAGTTC CACCTGANCC   841 GCCTGAACCT CCAGTACCGC TGGTGCTGCC NNCGGANTTG TACAGCTCGN NCNNGCCNAN   901 ANTGNTNCNN NCGGNNGNCA CGAACTCCNG CANGANCATG NNATCGCGCT TNNCNTTNGG   961 GGNCTTTGNN NNNNNCNNNN TNGGGNGCTN NNNNTAGTNG NTNGNNNGGN NNNNNCNNNG 1021 GGNNNNNNNN NN //
Primer: SV40pro_F_V2 (Pr0154) Region: KanR_NeoR Sequence file: RDB14204_A6Foi.seq
>D03467A2_A6Fo_1_SV40pro_F_V2_B03_06.ab1     1 NNNNNNNNNN NNGNNCNNNG CTTTTGCAAG NNCGNTCNAG AGACAGGNNG AGGNTCGTTT    61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA   121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG   181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA   241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT   301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG   361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA   421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT   481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC   541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC   601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA   661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA NCGCTATCAG   721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC   781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT   841 CTTGACGANT TCTTCTGANC GGGNNTCNNG GGTTCGAAAT GNNCGANNNA NCGACGCCCN   901 ACCTGCCATC ACGANATTTC NANTCCNCCG CCGCCNTCTA NNAANNNNTG GGCTTNNNNN 961 NNNNTTTNCN NGNNNNNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl