Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPAL7_GimRET

Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14203_A6Fop1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065) Region: lac O,RBS,Profinity eXact tag,ECFP 5' Sequence file: RDB14203_A6Fod.seq
>D03467A1_A6Fo_1_pBR322BamHI_R_G02_20.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNG GGNNNNNNTG AGCGGNNAAC AATTCCCCTC    61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGGAGGGA AATCAAACGG   121 GGAAAAGAAA TATATTGTCG GGTTCAAACA GGGCTTTAAG AGCTGCGCTA AGAAGGAGGA   181 TGTCATTTCT GAAAAAGGCG GGAAACTCCA AAAGTGCTTC AAATATGTAG ACGCAGCTAG   241 CGCTACATTA AACGAAAAAG CTGTAGAAGA ATTGAAAAAA GATCCGAGCG TCGCGTACGT   301 AGAAGAAGAC AAGCTCTTCA AAGCTTTGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT   361 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG   421 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG   481 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTGGGGCG TGCAGTGCTT   541 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG   601 CTACGTCCAG GANCGCACCA TCTTCTTCAA GGACGACNGC AACTACNAGA NCCGCGCCGA   661 NGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAANGGCA TCGACTTCAN   721 NGANGANGGC AACATCCTGG GGCACNANCT GNANTANANC TACNTCAGCN NNNACGTCTA   781 TATCNCCGCC GANNAGCNNA ANANNGGCAT NNAGGCNNNN TTCNNNATNC NNCCACNNNN   841 TCNNNGANNG NANNNNNGCA NNTNNNNNAN NNNTNCNNNN NNANNNNCCC CNNTCGGCGN 901 NGNNCCNNNN NNNNNN //
Primer: T7ter_R1 (Pr0798) Region: EYFP 3'(YFP1G) Sequence file: RDB14203_A6Foe.seq
>14203_14203_B1H2F_T7ter_R1_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTTGCTN NGCGGNGGCA GCNNGCCAAC TCAGCTTCCT    61 TTCGGGCTTA TGCGGCCGCC TCGAGTTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC   121 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC   181 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA   241 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC   301 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT   361 GGCTGTTGTA TCCGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT   421 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC   481 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG   541 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT   601 GCANGCCGTA NCCGAANGTG GTCACGAGGG TGGGCCAGGG NACGGGCAGC TTGNCGGTGG   661 TGCANANGNA NNTCNNNNNC AGCTNGNCGT NNNGGNATCN NCCNCNNCNN CNNNNGNANA 721 CNCNNAANTT NNGGNNNNTN NNNNNNNNCN NNNNN //
Primer: EYFP-H_R (Pr1036) Region: ECFP mid Sequence file: RDB14203_A6Fof.seq
>14203_14203_B1H2F_EYFP-H_R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTGG NGCTGCCTCC GGNCTTGTAC NGCTCGTCCA    61 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT   121 CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA   181 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT   241 CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG   301 TGATATAGAC GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT   361 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT   421 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA   481 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC   541 GGCTGAAGCA CTGCACGCCC CAGGTCANGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA   601 GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCNNCTTGCC GNNNNGGCAT CGCCCTCNCC 661 CTCNNCNGAC NNNCTNNNNN NNNNN //
Primer: ECFP-C_F1 (Pr0925) Region: ECFP 3',EYFP(YFP1G) 5' Sequence file: RDB14203_A6Fog.seq
>14203_14203_B1H2F_ECFP-C_F1_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNNNNN CNTGGTCCTG CTGGAGTTCG TGACCGCCGC    61 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA GGCAGCACCA GCGGTACTGG   121 AGGTTCAGGC GGCTCAGGTG GAACTGGTGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT   181 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG   241 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG   301 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCC TGCAGTGCTT   361 CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG   421 CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA   481 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA   541 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC GGATACAACA GCCACNACGT   601 CTATATCATG GCCGACAAGC AGAANAACNG CATCNAGGTG AACTTCNAGA NNCGCCNCNA   661 CATCGAGGAN NGCAGNNTGC ANCTCNNNGA NCNCTACCAG CAGANNACCC CNTCNGNCGA 721 NNNNCNNNNN NNNNN //
Primer: lacIpro_F3 (Pr0722) Region: lacI pro,lacI Sequence file: RDB14203_A6Foh.seq
>14203_14203_B1H2F_laclpro_F3_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA    61 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC   121 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG   181 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG   241 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA   301 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA   361 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC   421 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG   481 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG   541 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG   601 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCN TGAANANGGN ACGCGACTGG   661 GCGNNNNNCA TCTGNTCGCA TTGGGTCANC AGCAANTCGN GCTNNNNNCG GGNCNNNNNN 721 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl