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pPAL7_GimRET (#RDB14203)

Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Konishi, H.A., Sci. Rep. 7 (1): 5709 (2017). PMID 28720791. [PubMed] [Article] [RRC of NBRP]
Publication Morikawa, T.J., Sci. Rep. 6: 22342 (2016). PMID 26956628. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name CFP-YFP1G GimRET
Clone info. Expression vector of glycine-inserted mutant FRET (GimRET) fusion protein for E.coli. GimRET works as a protein-crowding sensor that consists of CFP with YFP1G (one glycine insertion).
Comment YFP1G (Inserted a Gly residue in the position of the 145th Tyr residue).
Vector backbone pAL7 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Watanabe, Tomonobu |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
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提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)。

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RDB14203 pPAL7_GimRET DNA solution

How to cite this biological resource

Materials & Methods section:

The pPAL7_GimRET was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14203).

Reference section:

Konishi, H.A., Asai, S., Watanabe, T.M., Yoshimura, S.H., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791. [PubMed] [Article] [RRC of NBRP]
Morikawa, T.J., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, T.M., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Konishi, H.A., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791. [PubMed] [Article] [RRC of NBRP]
original Morikawa, T.J., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628. [PubMed] [Article] [RRC of NBRP]
reference Machiyama, H., The use of a genetically encoded molecular crowding sensor in various biological phenomena. Biophys. Physicobiol. 14: 119-125 (2017). PMID 28900589. [PubMed] [Article]
review Watanabe, T.M., An intracellular crowding sensor based on fluorescent protein. (Japanese text) Journal of Japanese Biochemical Society 89 (4): 577-586 (2017). [Article]