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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-FLAG-Pemt-HA (#RDB14196)

Expression vector of human Pemt tagged with N-ter FLAG and C-ter HA in mammalian cells.

Clone info. Expression vector of human Pemt tagged with N-ter FLAG and C-ter HA in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3.1/V5-His-TOPO (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Pemt cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14196 pcDNA3.1-FLAG-Pemt-HA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-FLAG-Pemt-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14196).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14196_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14196_A6Faa.seq check
>D03475A7_A6Fa_1_CMV_Forward_B12_06.ab1
    1 CTTTACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGTTA AGCTTGCCAC CATGGACTAC AAGGACGACG
  181 ATGACAAAAA GAGATCTGGG AACCCGGGAG CCGAGGTAAC GAACAGCTCG GTGGCAGGGC
  241 CTGACTGCTG CGGAGGCCTC GGCAATATTG ATTTTAGACA GGCAGACTTC TGCGTTATGA
  301 CCCGGCTGCT GGGCTACGTG GACCCCCTGG ATCCCAGCTT TGTGGCTGCC GTCATCACCA
  361 TCACCTTCAA TCCGCTCTAC TGGAATGTGG TTGCACGATG GGAACACAAG ACCCGCAAGC
  421 TGAGCAGGGC CTTCGGATCC CCCTACCTGG CCTGCTACTC TCTAAGCGTC ACCATCCTGC
  481 TCCTGAACTT CCTGCGCTCG CACTGCTTCA CGCAGGCCAT GCTGAGCCAG CCCAGGATGG
  541 AGAGCCTGGA CACCCCCGCG GCCTACAGCC TGGGCCTCGC GCTCCTGGGA CTGGGCGTCG
  601 TGCTCGTGCT CTCCAGCTTC TTTGCACTGG GGTTCGCTGG AACTTTCCTA GGTGATTACT
  661 TCGGGATCCT CAAGGAGGCG AGAGTGACCG TGTTCCCCTT CAACATCCTG GACAACCCCA
  721 TGTACTGGGG AAGCACAGCC AACTACCTGG GCTGGGCCAT CATGCACGCC AGCCCCACGG
  781 GCCTGCTCCT GACGGTGCTG GTGGCCCTCA CCTACATAGT GGCTCTCCTA TACGAAGAGC
  841 CCTTCACCGC TGAGATCTAC CGGCAGAAAG CCTCCGGGGC CCCACAAGAG GAGCTACCCA
  901 TACGATGTTC CAGATTACGC TTGACTCGAG TCTAGAGGCC CGCGGTTCGA AAGGTAAGCC
  961 CTATCCCTAA ACCCTCTCCC TCGGTCTCGA TTCTACGCGT ACCGGTCATC ATCACCATCA
 1021 CCATTTGAGT TTAAAGCCCG CTTGAATCAG GCCTCGGACT GTGCATCCTA GCTGGCTAGG
1081 TCAATCTGAA TGTTTGGCCC CCATC
//
Primer: BGH_rev2
Sequence file: RDB14196_A6Fab.seq check
>D03475A7_A6Fa_1_BGH_rev2_C12_09.ab1
    1 GTACCTTTAG GAGGCACGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGGGTT TAAACTCAAT GGTGATGGTG ATGATGACCG GTACGCGTAG
  121 AATCGAGACC GAGGAGAGGG TTAGGGATAG GCTTACCTTC GAACCGCGGG CCCTCTAGAC
  181 TCGAGTCAAG CGTAATCTGG AACATCGTAT GGGTAGCTCC TCTTGTGGGG CCCGGAGGCT
  241 TTCTGCCGGT AGATCTCAGC GGTGAAGGGC TCTTCGTATA GGAGAGCCAC TATGTAGGTG
  301 AGGGCCACCA GCACCGTCAG GAGCAGGCCC GTGGGGCTGG CGTGCATGAT GGCCCAGCCC
  361 AGGTAGTTGG CTGTGCTTCC CCAGTACATG GGGTTGTCCA GGATGTTGAA GGGGAACACG
  421 GTCACTCTCG CCTCCTTGAG GATCCCGAAG TAATCACCTA GGAAAGTTCC AGCGAACCCC
  481 AGTGCAAAGA AGCTGGAGAG CACGAGCACG ACGCCCAGTC CCAGGAGCGC GAGGCCCAGG
  541 CTGTAGGCCG CGGGGGTGTC CAGGCTCTCC ATCCTGGGCT GGCTCAGCAT GGCCTGCGTG
  601 AAGCAGTGCG AGCGCAGGAA GTTCAGGAGC AGGATGGTGA CGCTTAGAGA GTAGCAGGCC
  661 AGGTAGGGGG ATCCGAAGGC CCTGCTCAGC TTGCGGGTCT TGTGTTCCCA TCGTGCAACC
  721 ACATTCCAGT AGAGCGGATT GAAGGTGATG GTGATGACGG CAGCCACAAA GCTGGGATCC
  781 AGGGGGTCCA CGTAGCCCAG CAGCCGGGTC ATAACGCAGA AGTCTGCCTG TCTAAAATCA
  841 ATATTGCCGA GGCCTCCGCA GCAGTCAGGC CCTGCCACCG AGCTGTTCGT TACCTCGGCT
  901 CCCGGGGTTC CCAGATCTCT TTTTTGTCAT CGTCGTCCTT TGTAGTCCAT GGGTGGCAAG
  961 CTTTAACTAG CCCAGCTTGG GTCTCCCTAT AGTGAGTCGT ATTTAAATTT CGATTAGGCC
 1021 AGGTAAGGCA GTGAGATTCT CTAGCTAAGT CAGAAGAGCT CTGCTTATAT AGACGTCCCA
1081 ACCGTACACG ACCGTAACCC GATCCCCT
//
Primer: SV40pro_F
Sequence file: RDB14196_A6Fac.seq check
>D03475A7_A6Fa_1_SV40pro_F_D12_12.ab1
    1 CCTGTCTTGA CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG GATTTCCGAT TCACGCGCGT TCTTATGAAA GGTGGCTCCG
 1021 CATTCGGTTT CCAGGACGCG CTTGGATGAT CTCAGCGCGG CGATCTCAAT GCTTGAAGTT
1081 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]