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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-p53-HA (#RDB14195)

Expression vector of human p53 tagged with C-ter HA in mammalian cells.

Clone info. Expression vector of human p53 tagged with C-ter HA in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3.1/V5-His-TOPO (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human p53 cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14195 pcDNA3.1-p53-HA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-p53-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14195).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14195_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14195_A6Faa.seq check
>D03475A6_A6Fa_2_CMV_Forward_G11_20.ab1
    1 CTTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGTTA AGCTTGCCAC CATGGAGGAG CCGCAGTCAG
  181 ATCCTAGCGT CGAGCCCCCT CTGAGTCAGG AAACATTTTC AGACCTATGG AAACTACTTC
  241 CTGAAAACAA CGTTCTGTCC CCCTTGCCGT CCCAAGCAAT GGATGATTTG ATGCTGTCCC
  301 CGGACGATAT TGAACAATGG TTCACTGAAG ACCCAGGTCC AGATGAAGCT CCCAGAATGC
  361 CAGAGGCTGC TCCCCGCGTG GCCCCTGCAC CAGCAGCTCC TACACCGGCG GCCCCTGCAC
  421 CAGCCCCCTC CTGGCCCCTG TCATCTTCTG TCCCTTCCCA GAAAACCTAC CAGGGCAGCT
  481 ACGGTTTCCG TCTGGGCTTC TTGCATTCTG GGACAGCCAA GTCTGTGACT TGCACGTACT
  541 CCCCTGCCCT CAACAAGATG TTTTGCCAAC TGGCCAAGAC CTGCCCTGTG CAGCTGTGGG
  601 TTGATTCCAC ACCCCCGCCC GGCACCCGCG TCCGCGCCAT GGCCATCTAC AAGCAGTCAC
  661 AGCACATGAC GGAGGTTGTG AGGCGCTGCC CCCACCATGA GCGCTGCTCA GATAGCGATG
  721 GTCTGGCCCC TCCTCAGCAT CTTATCCGAG TGGAAGGAAA TTTGCGTGTG GAGTATTTGG
  781 ATGACAGAAA CACTTTTCGA CATAGTGTGG TGGTGCCCTA TGAGCCGCCT GAGGTTGGCT
  841 CTGACTGTAC CACCATCCAC TACAACTACA TGTGTAACAG TTCCTGCATG GGCGGCATGA
  901 ACCGGAGGCC CATCCTCACC ATCATCACAC TGGAAAGACT CCAGTGGGTA ATCTACTGGG
  961 GACGGGAACA GCTTTTGAGG TGCGTGTTTG TGCCCTGTCC CTGGGAAGAG ACCGGGCGCA
 1021 CAGAGGAGAG ATCCTCGCCA GAAATGGGGA GCTCACACGA AGCCTGGCCC CCCACGGGAG
1081 CCAACCTATG
//
Primer: BGH_rev2
Sequence file: RDB14195_A6Fab.seq check
>D03475A6_A6Fa_2_BGH_rev2_H11_23.ab1
    1 GACGTAGTAG CACGGGGGAG GGGCAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGGGTTTAAA CTCAATGGTG ATGGTGATGA TGACCGGTAC GCGTAGAATC
  121 GAGACCGAGG AGAGGGTTAG GGATAGGCTT ACCTTCGAAC CGCGGGCCCT CTAGACTCGA
  181 GTCAAGCGTA ATCTGGAACA TCGTATGGGT AGTCTGAGTC AGGCCCTTCT GTCTTGAACA
  241 TGAGTTTTTT ATGGCGGGAG GTAGACTGAC CCTTTTTGGA CTTCAGGTGG CTGGAGTGAG
  301 CCCTGCTCCC CCCTGGCTCC TTCCCAGCCT GGGCATCCTT GAGTTCCAAG GCCTCATTCA
  361 GCTCTCGGAA CATCTCGAAG CGCTCACGCC CACGGATCTG AAGGGTGAAA TATTCTCCAT
  421 CCAGTGGTTT CTTCTTTGGC TGGGGAGAGG AGCTGGTGTT GTTGGGCAGT GCTCGCTTAG
  481 TGCTCCCTGG GGGCAGCTCG TGGTGAGGCT CCCCTTTCTT GCGGAGATTC TCTTCCTCTG
  541 TGCGCCGGTC TCTCCCAGGA CAGGCACAAA CACGCACCTC AAAGCTGTTC CGTCCCAGTA
  601 GATTACCACT GGAGTCTTCC AGTGTGATGA TGGTGAGGAT GGGCCTCCGG TTCATGCCGC
  661 CCATGCAGGA ACTGTTACAC ATGTAGTTGT AGTGGATGGT GGTACAGTCA GAGCCAACCT
  721 CAGGCGGCTC ATAGGGCACC ACCACACTAT GTCGAAAAGT GTTTCTGTCA TCCAAATACT
  781 CCACACGCAA ATTTCCTTCC ACTCGGATAA GATGCTGAGG AGGGGCCAGA CCATCGCTAT
  841 CTGAGCAGCG CTCATGGTGG GGGGCAGCGC CTCACAACCT CCGTCATGTG CTGTGACTGC
  901 TTGTAGATGG CCATGGCGCG GACGCGGGTG CCGGGCGGGG GTGTGGAATC AACCCACAGC
  961 TGCACAGGCA GGTCTTGGCC CAGTTGGGCA AAACATCTTT GTTTGAGGCA GGGAGTACGT
 1021 GCAAGTCCAC AGACTTTGGG CTGTCCCAGA ATGCAGCAGT CCAGGACGAA ACTCGTAGCT
1081 GCCCCTGGCT AGGAT
//
Primer: SV40pro_F
Sequence file: RDB14195_A6Fac.seq check
>D03475A6_A6Fa_2_SV40pro_F_A12_03.ab1
    1 CACCGGTCTG GACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAAACCT GCCATCACGA GATTTCGATT TCCACCGCCG CCCTTTCTAT GGAAAGATGG
 1021 GCTTCGGAAT TCGGTTTCAG GACGCCGGCC TGGGATGATC CATCCAGCGC GGAGAATCTC
1081 ATGGCTTGGA AAGATTCAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]