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pcDNA3-myc-CHC(1-1676) (#RDB14194)

Expression vector of myc-tagged full coding cDNA of human CHC (1-1676) in mammalian cells.

Clone info. Expression vector of myc-tagged full coding cDNA of human CHC (1-1676) in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human CHC cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14194 pcDNA3-myc-CHC(1-1676) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-myc-CHC(1-1676) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14194).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14194_A6Fap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,Myc,insert 5'
Sequence file: RDB14194_A6Fad.seq check
>D03475A5_A6Fa_2_CMV_Forward_D11_11.ab1
    1 NNNNNNNNNN NNNNNNTNGN NGCNNNTGGG CGGTANGCGT GTACGGTGGG NNGTCTATAT
   61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
  121 GACTCACTAT AGGGAGACCC AAGCTTGGTA CCGAGCTCGG ATCCACCATG GAGCAAAAGC
  181 TAATATCTGA AGAGGACCTA GAATTCTGCA GATATCCATC ACACTGGCGG CCGCCCATGG
  241 CCCAGATTCT GCCAATTCGT TTTCAGGAGC ATCTCCAGCT CCAGAACCTG GGTATCAACC
  301 CAGCAAACAT TGGCTTCAGT ACCCTGACTA TGGAGTCTGA CAAATTCATC TGCATTAGAG
  361 AAAAAGTAGG AGAGCAGGCC CAGGTGGTAA TCATTGATAT GAATGACCCA AGTAATCCAA
  421 TTCGAAGACC AATTTCAGCA GACAGCGCCA TCATGAATCC AGCTAGCAAA GTAATTGCAC
  481 TGAAAGCTGG GAAAACTCTT CAGATTTTTA ACATTGAAAT GAAAAGTAAA ATGAAGGCTC
  541 ATACCATGAC TGATGATGTC ACCTTTTGGA AATGGATCTC TTTGAATACG GTTGCTCTTG
  601 TTACGGATAA TGCAGTTTAT CACTGGAGTA TGGAAGGAGA GTCTCAGCCA GTGAAAATGT
  661 TTGATCGCCA TTCTAGCCTT GCAGGGTGCC AGATTATCAA TTACCGTACA GATGCAAAAC
  721 AAAAGTGGTT ACTTCTGACT GGTATATCTG CACAGCAAAA TCGTGTGGTG GGAGCTATGC
  781 AGCTATATTC TGTAGATAGG AAAGTGTCTC AGCNCATTGA ANGANATGCA GCTAGCNNNG
  841 CACAGTTTAA GATGGAAGGA AATGCAGAAG ANNNAACGTT ATTTTTGTTT TGCANTTCGG
  901 GGNCNNNNCT GGNNGGNANT TNNNTATTAN NGNANNNTNG NNNNNNNNNC NNNNNNNNAN
961 NNNNCCNNTT NNCNNANNNA NNNNNNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: bGHpA,insert 3'
Sequence file: RDB14194_A6Fae.seq check
>D03475A5_A6Fa_2_BGH_rev2_C01_07.ab1
    1 NNNNNNNNNN NNNNNNGGGG GNNGGGCNNA CNACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCACATG
  121 CTGTACCCAA AGCCAGGCTG TGGCTGTCCA TACGGTGGTG CGGTATAACC ATAACCAAAA
  181 GGTGCCTGGG GAGGGACGGC AACACTGGGT CCTGCTGTCA GCATCAACTG GGGCTGACCA
  241 TAAACAATGG GTTGTGTCTC TGTAGCTTGT TCTTCTTCTT TTCTCAGTGA TTCTGAAGCA
  301 TCTAATTTAT CCACCTTTGT CAAGTACTCC TTCATGACCT GGATGAAATA GGGCATGGCA
  361 AAATCCATGA TATTGTGCCT CCATGCAGTT TCTAGGACGA CATCTGGCCT TAAAAGATCG
  421 TAACAGGTAA ACAGACAAGC TCCAAAGCAC TCTCTTTTTT CTTCCTGCAA AAACCACTGC
  481 AGGAGTTCTT CAGCCAATTC AGTATCTTTA GATTCAGAAG CATACTGCAT TGCATCCTTG
  541 TAAAGGCTGT CTTTCTTGCA CAGCTCTACA CTCTGTTTCC AGCGATTGTT GCCTTTGAAG
  601 AGATAAGCAG CAATTCTCCT GAACTCAATG AGTTCATGTT TTTCCAAACG CTGAGCAAGC
  661 GAGATATTGT CAAAGTTGTC ATAAGCATCT ATTGATGTTC GCAGAGCCTG ATAATCTTCT
  721 TCTGTAATAA AAAGATTGTT CAATGATTCA TTCACAGATT TGTTGTTATG GTTCTGAACT
  781 GAACGCAAAT ACGGTTTCAC CNGTGGTAGC TGTTTAACCT TGCTGAAATA ATTGACTGCA
  841 CGANTGTGAT NCAACCGTGG ANACAGCACC NTCAGCNAAT CATTTNACNA CNGANNNTNN
  901 AATTNNAAGT ANAANNGTAN NGNNCNGNAN TATANTTNCN NNTTNGCAAN CNNNNGNNNN
961 GANNNNNTTN NANNNGNNNN NNNTTNNNNN NNNNN
//
Primer: SV40pro_F (Pr0110)
Region: NeoR
Sequence file: RDB14194_A6Faf.seq check
>D03475A5_A6Fa_2_SV40pro_F_D01_10.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNANNNGTGA GGNNGNTTTT TTGGANGNCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATNTCNNTT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC
  781 TATCANGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG ANCTTGGCGG CGAATGGGCT
  841 GANCGCTTCC TCGNGCTTTA CGGTATCGCC GCNNCCGANN CNCAGCGCAT CGCCTTCNAT
  901 CGCCNNCTTG NNNANTTCTT NTGANCGGGN NTCTNGGGGT TCNAAATGAN CGACCNAGCG
961 NNGCCNNANN NNGNNNTCAC NANANTNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]