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pcDNA3-myc-CHC(1267-1513) (#RDB14193)

Expression vector of myc-tagged partial coding cDNA of human CHC (1267-1513) in mammalian cells.

Clone info. Expression vector of myc-tagged partial coding cDNA of human CHC (1267-1513) in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human CHC cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14193 pcDNA3-myc-CHC(1267-1513) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-myc-CHC(1267-1513) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14193).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14193_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14193_A6Faa.seq check
>D03475A4_A6Fa_1_CMV_Forward_A11_02.ab1
    1 ACTATAACCT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGAGCTC GGATCCACCA TGGAGCAAAA
  181 GCTAATATCT GAAGAGGACC TAGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCCG
  241 TCTTGCTCAG ATGTGTGGAC TTCATATTGT TGTACATGCA GATGAATTAG AAGAACTTAT
  301 CAACTACTAT CAGGATCGTG GCTATTTTGA AGAGCTGATC ACCATGTTGG AAGCAGCACT
  361 GGGACTTGAG CGAGCTCACA TGGGAATGTT TACTGAATTA GCTATTCTAT ACTCTAAATT
  421 TAAGCCTCAG AAAATGAGGG AGCACCTGGA GCTGTTCTGG TCTAGAGTGA ATATTCCCAA
  481 GGTGCTAAGA GCTGCAGAAC AAGCTCATCT TTGGGCAGAA CTGGTGTTTT TGTATGACAA
  541 GTATGAAGAA TATGATAATG CCATAATTAC CATGATGAAT CATCCAACTG ATGCCTGGAA
  601 AGAAGGGCAA TTCAAAGATA TCATTACCAA GGTTGCCAAT GTGGAACTAT ACTACAGAGC
  661 AATACAGTTC TACTTAGAAT TCAAGCCTCT GTTGTTAAAT GATTTGCTGA TGGTGCTGTC
  721 TCCACGGTTG GATCACACTC GTGCAGTCAA TTATTTCAGC AAGGTTAAAC AGCTACCACT
  781 GGTGAAACCG TATTTGCGTT CAGTTCAGAA CCATAACAAC AAATCTGTGA ATGAATCATT
  841 GAACAATCTT TTTTATTACA GAAGAAGATT ATCAGGCTCT GCGAACATCA ATAGATGCTT
  901 ATGACAACTT TTGACAATAT CTCGCTTGCT CAGCGTTTGG AAAAAACATG AACTCATTGA
  961 GTTCACGAGA ATTGCTGCTT AATTAACGGC CCTATTTCTA TAGTGTCACC CTAAAATGCC
 1021 TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCAATCT GGTTGTTGGC
1081 CTCCTTCCCC CCGGTGGCCC TCTTCCCTTG TGAC
//
Primer: BGH_rev2
Sequence file: RDB14193_A6Fab.seq check
>D03475A4_A6Fa_1_BGH_rev2_B11_05.ab1
    1 GACGGTAGTA GCACGGGGGA GGGGCAACAA CAGATGGCTG GCAACTAGAA GGCACAGTCG
   61 AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC TTAATAAGCA
  121 GCAATTCTCC TGAACTCAAT GAGTTCATGT TTTTCCAAAC GCTGAGCAAG CGAGATATTG
  181 TCAAAGTTGT CATAAGCATC TATTGATGTT CGCAGAGCCT GATAATCTTC TTCTGTAATA
  241 AAAAGATTGT TCAATGATTC ATTCACAGAT TTGTTGTTAT GGTTCTGAAC TGAACGCAAA
  301 TACGGTTTCA CCAGTGGTAG CTGTTTAACC TTGCTGAAAT AATTGACTGC ACGAGTGTGA
  361 TCCAACCGTG GAGACAGCAC CATCAGCAAA TCATTTAACA ACAGAGGCTT GAATTCTAAG
  421 TAGAACTGTA TTGCTCTGTA GTATAGTTCC ACATTGGCAA CCTTGGTAAT GATATCTTTG
  481 AATTGCCCTT CTTTCCAGGC ATCAGTTGGA TGATTCATCA TGGTAATTAT GGCATTATCA
  541 TATTCTTCAT ACTTGTCATA CAAAAACACC AGTTCTGCCC AAAGATGAGC TTGTTCTGCA
  601 GCTCTTAGCA CCTTGGGAAT ATTCACTCTA GACCAGAACA GCTCCAGGTG CTCCCTCATT
  661 TTCTGAGGCT TAAATTTAGA GTATAGAATA GCTAATTCAG TAAACATTCC CATGTGAGCT
  721 CGCTCAAGTC CCAGTGCTGC TTCCAACATG GTGATCAGCT CTTCAAAATA GCCACGATCC
  781 TGATAGTAGT TGATAAGTTC TTCTAATTCA TCTGCATGTA CAACAATATG AAGTCCACAC
  841 ATCTGAGCAA GACGGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC TAGGTCCTCT
  901 TCAGATATTA GCTTTTTGCT CCATGGTGGA TCCGAGCTCG GTACCAAGCT TGGGTCTCCC
  961 TATAGTGAGT CGTATTAATT TCGATAAGCC CAGTAAGCAG TGGGGTTCTC TAGTTTAGCC
 1021 CAGAGAGCTC TGCTTATAAT AGACCTTCCC CACCGTTACA CGCATACGAC CCAATTTGCG
1081 TCAATGGGGG CGGCAGTTTG CTTAACCGGA CT
//
Primer: SV40pro_F
Sequence file: RDB14193_A6Fac.seq check
>D03475A4_A6Fa_1_SV40pro_F_C11_08.ab1
    1 ACTGGTCTGG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTTC GAAATGACCG ACCCAAGCGA
  961 CGCCCAAACC CTGCCCATCA CGAGATTTCC GATTTCACGC CGCCTTCCTA TGGAAACGAT
 1021 GGCTTTCCGG ATCGTTTTCA GACGCGGCCT TGCATGATCC TCCAAGCGCG GGAATCTCAT
1081 GCCTTGCAGT C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]