Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-myc-CHC(1074-1406) (#RDB14192)

Expression vector of myc-tagged partial coding cDNA of human CHC (1074-1409) in mammalian cells.

Clone info. Expression vector of myc-tagged partial coding cDNA of human CHC (1074-1409) in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human CHC cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14192 pcDNA3-myc-CHC(1074-1406) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-myc-CHC(1074-1406) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14192).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14192_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14192_A6Faa.seq check
>D03475A3_A6Fa_2_CMV_Forward_F10_16.ab1
    1 CTTTCACTCG CCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CCACCATGGA GCAAAAGCTA
  181 ATATCTGAAG AGGACCTAGA ATTCTGCAGA TATCCATCAC ACTGGCGGCC GCGGAAATTT
  241 GATGTCAATA CTTCAGCAGT TCAGGTCTTA ATTGAGCATA TTGGAAACTT GGATCGGGCA
  301 TATGAGTTTG CTGAACGTTG CAATGAACCT GCGGTCTGGA GTCAACTTGC AAAAGCCCAG
  361 TTGCAGAAAG GAATGGTGAA AGAAGCCATT GATTCTTATA TCAAAGCAGA TGATCCTTCC
  421 TCCTACATGG AAGTTGTTCA GGCTGCCAAT ACTAGTGGAA ACTGGGAAGA ACTGGTGAAG
  481 TACTTGCAGA TGACCCGTAA GAAGGCTCGA GAGTCCTATG TGGAGACAGA ACTGATATTC
  541 GCACTGGCTA AAACAAACCG CCTTGCAGAG TTAGAAGAAT TTATCAATGG ACCAATAATG
  601 CTCATATCCA ACAAGTTGGT GACCGTTGTT ATGATGAAAA AATGTATGAT GCTGCTAAGT
  661 TGTTTGTACA ATAATGTTTC CAATTTTGGA CGTTTGGCAT CTACCTGGTC ACCTGGGGTG
  721 AATATTCAGC AGCTTGTTTG ATGGGGCTAG AAAGCTTAAC AGTTCCTCGA ACATGAAAAG
  781 AGGTCTCTGC TTCTCCCCGT TGGTTAATGG GAAAGATCGC TTGTCTCAAT GTGTGGAACT
  841 TCATATTTGG TGTGCACTGG CGAATGAGAT CGAGAACTTC AACTACTATT CGGAGTCTGG
  901 CATTGGAGGC TGTATCCATG TGAGCCCTTG GACTAGCCAT CTAGTGAAGT CGGAATCCTC
961 TATCGA
//
Primer: BGH_rev2
Sequence file: RDB14192_A6Fab.seq check
>D03475A3_A6Fa_2_BGH_rev2_G10_19.ab1
    1 GTGTGGGGCC AGCAGCCGGG GGAGGGGCAA CAACAGATGG CTGGCAACTA GAAGGCACAG
   61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTTATCTG
  121 TAGTATAGTT CCACATTGGC AACCTTGGTA ATGATATCTT TGAATTGCCC TTCTTTCCAG
  181 GCATCAGTTG GATGATTCAT CATGGTAATT ATGGCATTAT CATATTCTTC ATACTTGTCA
  241 TACAAAAACA CCAGTTCTGC CCAAAGATGA GCTTGTTCTG CAGCTCTTAG CACCTTGGGA
  301 ATATTCACTC TAGACCAGAA CAGCTCCAGG TGCTCCCTCA TTTTCTGAGG CTTAAATTTA
  361 GAGTATAGAA TAGCTAATTC AGTAAACATT CCCATGTGAG CTCGCTCAAG TCCCAGTGCT
  421 GCTTCCAACA TGGTGATCAG CTCTTCAAAA TAGCCACGAT CCTGATAGTA GTTGATAAGT
  481 TCTTCTAATT CATCTGCATG TACAACAATA TGAAGTCCAC ACATCTGAGC AAGACGGAAT
  541 TCTTTCCCAT CTACACAGGC GAAGCAGACC TCTTTCCATG TTCGAGTACT GTTAGCTTTC
  601 CTAGCCCCAT CAACAGCTGC CTGATATTCA CCCAGGTGAA CCAGGGTAGA TGCCAAACGT
  661 CCAAAATTGG AAACATTATT GTACAACAAC TTAGCAGCAT CATACATTTT TTCATCATAA
  721 CAACGGTCAC CAACTTGTTG GATATGAGCA TTATTTGGTC CATTGATAAA TTCTTCTAAC
  781 TCTGCAAGGC GGTTTGTTTT AGCCAGTGCG AATATCAGTT CTGTCTCCAC ATAGGACTCT
  841 CGAGCCTTCT TACGGGTCAT CTGCAAGTAC TTCACCAGTT CTTCCCAGTT TCCACTAGTA
  901 TTGGCAGCCT GAACAACTTC CATGTAGGAG GAAGGATCAT CTGCTTTGAT ATAAGAATCA
  961 ATGGGCTTTC TTTCACCATT TCCTTTCTGC AACTGGGCTT TTGCAAGGTT GAACTCAGAA
 1021 CCGGCAGATC AATTGGCCAC CGATCCAGCC AACTTCATAT GCCGATCAAG TTTCATATGC
1081 TTCAATTTAA GG
//
Primer: SV40pro_F
Sequence file: RDB14192_A6Fac.seq check
>D03475A3_A6Fa_2_SV40pro_F_H10_22.ab1
    1 CACGTCTGTG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
   61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGGACTC TGGGGTTCGA AATGACCGAC CCAAGCGACG
  961 CCCAAACCTG CCCATCACGA GAATTTCCGA TTTCCACCGT CGCCTTCTAT GAAAAGTTTG
 1021 GCCTTCGGAA TCGTTTTTCC CGGGAACGTC TGCCTTGGAT GATTCTCAGG CGCGGGGGAA
1081 TCTCATGGCT GGGAAGTTTC ATTCCCGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]