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pcDNA3-myc-CHC(232-340) (#RDB14191)

Expression vector of myc-tagged partial coding cDNA of human CHC (232-340) in mammalian cells.

Clone info. Expression vector of myc-tagged partial coding cDNA of human CHC (232-340) in mammalian cells.
Comment Expression was confirmed by the depositor with luciferase reporter assay.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human CHC cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14191 pcDNA3-myc-CHC(232-340) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-myc-CHC(232-340) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14191).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14191_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14191_A6Faa.seq check
>D03475A2_A6Fa_2_CMV_Forward_D10_10.ab1
    1 CTTTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACCATGGAG CAAAAGCTAA
  181 TATCTGAAGA GGACCTAGAA TTCTGCAGAT ATCCATCACA CTGGCGGCCG CTTGAAGTTG
  241 GCACACCACC TACAGGGAAC CAGCCCTTTC CAAAGAAGGC AGTGGATGTC TTCTTTCCTC
  301 CAGAAGCACA AAATGATTTT CCTGTTGCAA TGCAGATCAG TGAAAAGCAT GATGTGGTGT
  361 TCTTGATAAC CAAGTATGGT TATATCCACC TCTATGATCT TGAGACTGGT ACCTGCATCT
  421 ACATGAATAG AATCAGTGGA GAAACAATTT TTGTTACTGC ACCTCATGAA GCCACAGCTG
  481 GAATAATTGG AGTAAACAGA AAGGGACAAG TTCTGTCAGT GTGTGTGGAA GAAGAAAACA
  541 TAATTCCTTA CATCACCAAT TAAGGGCCCT ATTCTATAGT GTCACCTAAA TGCTAGAGCT
  601 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC
  661 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA
  721 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC
  781 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG
  841 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC
  901 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC
  961 GCCCTAGCGC CCGCTCCCTT TCGCTTTCTT CCCTTTCCTT TTCTCGCCAC GTTTCGCAGC
 1021 TTTCCCCCGT CAGGCTCTAA TTCGGGGGGC TCCCTTTATG ATCCGAATTA GTGCCTTTAC
1081 CGGCCAATGA TCCGAAGTC
//
Primer: SV40pro_F
Sequence file: RDB14191_A6Fab.seq check
>D03475A2_A6Fa_2_SV40pro_F_E10_13.ab1
    1 CACCGGTCGG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGGCG AATGGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGGATT CGGCAGCGCA TCGGCCTTCT
  901 ATCGCCTTCT TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTTCGAAA TGACCCGACC
  961 AAGCGGACGC CCACCTGCCA TCACGGAGAT TTTCGAATTC CAACCGCCGG CCTTTCTTAT
 1021 GAAAGGATGG CCTCGGAATT CGCTTTCCGG AACGGCGGCT GGATGAATCC TCAGCGCGGG
1081 GAATCTCATG CCTGGGAGGT TCCTTTTCCG GCCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]