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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3xFLAG-CMV-10 Pemt (#RDB14190)

Expression vector of FLAG-tagged full coding cDNA of human Pemt in mammalian cells.

Clone info. Expression vector of FLAG-tagged full coding cDNA of human Pemt in mammalian cells.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone p3xFLAG-CMV-10 Expression Vector (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Pemt cDNA
Depositor|Developer Wada, Jun |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatsuka, A. et al., Sci. Rep. 6:21721, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14190 p3xFLAG-CMV-10 Pemt DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3xFLAG-CMV-10 Pemt was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14190).

Reference section:

Nakatsuka, A., Matsuyama, M., Yamaguchi, S., Katayama, A., Eguchi, J., Murakami, K., Teshigawara, S., Ogawa, D., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Watanabe, E., Wada, J., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14190_A6Fap1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14190_A6Faa.seq check
>D03475A1_A6Fa_2_CMV_Forward_A10_01.ab1
    1 CTTATACCCG CCCGTTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCGT TTAGTGAACC GTCAGAATTA ACCATGGACT ACAAAGACCA TGACGGTGAT
  121 TATAAAGATC ATGACATCGA TTACAAGGAT GACGATGACA AGCTTGCGGC CGCGAATTCA
  181 ATGAAGAGAT CTGGGAACCC GGGAGCCGAG GTAACGAACA GCTCGGTGGC AGGGCCTGAC
  241 TGCTGCGGAG GCCTCGGCAA TATTGATTTT AGACAGGCAG ACTTCTGCGT TATGACCCGG
  301 CTGCTGGGCT ACGTGGACCC CCTGGATCCC AGCTTTGTGG CTGCCGTCAT CACCATCACC
  361 TTCAATCCGC TCTACTGGAA TGTGGTTGCA CGATGGGAAC ACAAGACCCG CAAGCTGAGC
  421 AGGGCCTTCG GATCCCCCTA CCTGGCCTGC TACTCTCTAA GCGTCACCAT CCTGCTCCTG
  481 AACTTCCTGC GCTCGCACTG CTTCACGCAG GCCATGCTGA GCCAGCCCAG GATGGAGAGC
  541 CTGGACACCC CCGCGGCCTA CAGCCTGGGC CTCGCGCTCC TGGGACTGGG CGTCGTGCTC
  601 GTGCTCTCCA GCTTCTTTGC ACTGGGGTTC GCTGGAACTT TCCTAGGTGA TTACTTCGGG
  661 ATCCTCAAGG AGGCGAGAGT GACCGTGTTC CCCTTCAACA TCCTGGACAA CCCCATGTAC
  721 TGGGGAAGCA CAGCCAACTA CCTGGGCTGG GCCATCATGC ACGCCAGCCC CACGGGCCTG
  781 CTCCTGACGG TGCTGGTGGC CCTCACCTAC ATAGTGGCTC TCCTATACGA AGAGCCCTTC
  841 ACCGCTGAGA TCTACCGGCA GAAAGCCTCC GGGCCCCACA AGAGGAGCTA ATCTAGAGGA
  901 TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AGTTGCCACC
  961 TCCAGTGCCC CACCAGCCCT GTCTAATAAA ATAGTGCATC AATTTGGTCT GACTAAGGTG
 1021 TCGTTCTATA ATATTATGAG TTGAAGGAGT GGTATGAGCC AAGGGTAAGT TGGAGAACAA
1081 T
//
Primer: SV40pro_F_V2
Sequence file: RDB14190_A6Fab.seq check
>D03475A1_A6Fa_2_SV40pro_F_V2_C10_07.ab1
    1 GAGCTTTTGG AGATTGATCA GCTTGGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG
   61 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  121 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  181 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  241 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  301 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  361 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  421 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  481 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  541 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA
  601 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  661 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA
  721 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
  781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
  841 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTCGAAAT GACCGACCCA AGCGACGCCC
  901 AAACCTGCAA TCACGAGATT TCGATTTCCA CCGGCCGCCT TCCTATGAAC GTGGGCCTTC
961 GATTCGTTTC CAGACGCTGG CTGGATGATC TCAAGCGCCG GGGAATCCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakatsuka, A., Insufficiency of phosphatidylethanolamine N-methyltransferase is risk for lean non-alcoholic steatohepatitis. Sci. Rep. 6: 21721 (2016). PMID 26883167. [link to RRC of NBRP]