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human MLC20 T18A+S19A

Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A/S19A).

Catalog number RDB14161
Resource name human MLC20 T18A+S19A
Clone info. Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A/S19A).
Expression was confirmed by the depositor with immunoblot and microphotographs.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name Human MYL12B cDNA
Depositor Hirano, Katsuya |
 
Other clones in our bank human MYL12B (NCBI Gene 103910) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, M. et al., Sci. Rep. 6:20989, 2016)
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Remarks Remember that you will be working with samples containing infectious virus.
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RDB14161 human MLC20 T18A+S19A DNA solution

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References and tips

Electronic file

Featured content

References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14161_A6Epp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14161_A6Epa.seq
>D03455A4_A6Ep_pAxCA_F1_G09_21.ab1
    1 AAACAGGGTT TCCGGGGTTC ATGTTCATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA
   61 ACGTGCTGGT TATTGTGCTG TTCTCATCAT TTTGGCAAAG AATTGATTTA ATCGATTTGA
  121 TCCCCACCAT GTCGAGCAAA AAGGCAAAGA CCAAGACCAC CAAGAAGCGC CCTCAGCGTG
  181 CAGCAGCCAA TGTGTTTGCC ATGTTTGACC AGTCACAGAT TCAGGAGTTC AAAGAGGCCT
  241 TCAACATGAT TGATCAGAAC AGAGATGGCT TCATCGACAA GGAAGATTTG CATGATATGC
  301 TTGCTTCTCT AGGGAAGAAT CCCACTGATG CATACCTTGA TGCCATGATG AATGAGGCCC
  361 CAGGGCCCAT CAATTTCACC ATGTTCCTGA CCATGTTTGG TGAGAAGTTA AATGGCACAG
  421 ATCCTGAAGA TGTCATCAGA AACGCCTTTG CTTGCTTTGA TGAAGAAGCA ACAGGCACCA
  481 TTCAGGAAGA TTACCTAAGA GAGCTGCTGA CAACCATGGG GGATCGGTTT ACAGATGAGG
  541 AAGTGGATGA GCTGTACAGA GAAGCACCTA TTGACAAAAA GGGGAATTTC AATTACATCG
  601 AGTTCACACG CATCCTGAAA CATGGAGCCA AAGACAAAGA TGACTGAAAG AACTTTAGCT
  661 AAAAGGGCCC GCGAAATTAA TTAAACTAGT CTAGAATCGA TAATCAATTC ACTCCTCAGG
  721 TGCAGGCTGC CTATCAGAAG GTGGTGGCTG GGTGTGGCCA ATGCCCCTGG CTCACAAATA
  781 CCACTGAGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC ATCATGAAGC CCCTTTGAGC
  841 ATCTGACTTC TGGCTAATAA AGGAAAATTT ATTTTCATTG CATAGTGTGT TGGAATTTTT
  901 TTGTGTCTCT CACTCGGGAA GGACATATGG GAAGGGCAAA TCATTTAAAA CATCAGAATG
  961 AGTATTTGGG TTTAGAGTTT TTGGCCACAT ATGCCCATAT GCTGGCTGCC ATGGAACAAA
 1021 GGGTTGGCTT ATAAAAAAGG TCATCAGTTA TATGAAACCA AGCCCCCCCT GGCTTGTCCA
 1081 ATTCCCTTAT TTCCCATAGA AAAGCCCTTG ACCTTTGGAG GTTTAGATTT TTTTTTCATA
 1141 CTATTTGGTG TTGTGGTTTA ATTGTGCGTG AGCGAAGTCC CCTAAATTTG CGTTACCTGT
1201 TTACCATAGC CAGCAAATAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl