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human MLC20 S19A

Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, S19A).

Catalog number RDB14160
Resource name human MLC20 S19A
Clone info. Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, S19A).
Expression was confirmed by the depositor with immunoblot and microphotographs.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name Human MYL12B cDNA
Depositor Hirano, Katsuya |
 
Other clones in our bank human MYL12B (NCBI Gene 103910) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, M. et al., Sci. Rep. 6:20989, 2016)
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Remarks Remember that you will be working with samples containing infectious virus.
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RDB14160 human MLC20 S19A DNA solution

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References and tips

Electronic file

Featured content

References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14160_A6Epp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_R1 RDB14160_A6Epa.seq
>D03455A3_A6Ep_pAxCA_F1_C12_09.ab1
    1 CCCTTTTCTT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC TGGTTATTGT GCTGTCTCAT
   61 CATTTTGGCA AAGAATTGAT TTATCGATTT CGCGGGCCCC CATGTCGAGC AAAAAGGCAA
  121 AGACCAAGAC CACCAAGAAG CGCCCTCAGC GTGCAACAGC CAATGTGTTT GCCATGTTTG
  181 ACCAGTCACA GATTCAGGAG TTCAAAGAGG CCTTCAACAT GATTGATCAG AACAGAGATG
  241 GCTTCATCGA CAAGGAAGAT TTGCATGATA TGCTTGCTTC TCTAGGGAAG AATCCCACTG
  301 ATGCATACCT TGATGCCATG ATGAATGAGG CCCCAGGGCC CATCAATTTC ACCATGTTCC
  361 TGACCATGTT TGGTGAGAAG TTAAATGGCA CAGATCCTGA AGATGTCATC AGAAACGCCT
  421 TTGCTTGCTT TGATGAAGAA GCAACAGGCA CCATTCAGGA AGATTACCTA AGAGAGCTGC
  481 TGACAACCAT GGGGGATCGG TTTACAGATG AGGAAGTGGA TGAGCTGTAC AGAGAAGCAC
  541 CTATTGACAA AAAGGGGAAT TTCAATTACA TCGAGTTCAC ACGCATCCTG AAACATGGAG
  601 CCAAAGACAA AGATGACTGA AAGAACTTTA GCTAAAAGGG ATCAAATTAA TTAAACTAGT
  661 CTAGAATCGA TAATCAATTC ACTCCTCAGG TGCAGGCTGC CTATCAGAAG GTGGTGGCTG
  721 GTGTGGCCAA TGCCCTGGCT CACAAATACC ACTGAGATCT TTTTCCCCTC TGCCAAAAAT
  781 TATGGGGACA TCATGGAAAG CCCCCTTAAC ATCTCTGACA TTCTCTGCTC TAAATAAAAG
  841 AAAATTTTTA TTTTCTCATT TCGCAATAGT GTGTTTGAGA ATTTTTTTTG TGTCTTCTCA
  901 CTCGGAAAGA CATATGGGGA GGGCAAATTC ATTTAAAACA TCAGATGATA TTGTTTAGAG
  961 TTTGGCACAT ATGCCCATAT GCTGGCTGCC ACATATGCTG CTTGCCAATG AAACAAAGGT
 1021 TTGGCTATAA AGAGAAGGTT CCAATCAGTA ATTATGGAAA CCAGCCCCCC TGCCTTGTCA
1081 TCCGTTACTC GATAGGAATA GTTGATAGTC TAGAATTTTT TTAATAGTG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

2018.12.10

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