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human MLC20 T18A

Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A).

Catalog number RDB14159
Resource name human MLC20 T18A
Clone info. Shuttle vector to generate recombinant adenovirus expressing human MYL12B (mutant, T18A).
Expression was confirmed by the depositor with immunoblot and microphotographs.
Vector backbone pAxCAwtit (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45 kb
Selectable markers Amp^r
Gene/insert name Human MYL12B cDNA
Depositor Hirano, Katsuya |
 
Other clones in our bank human MYL12B (NCBI Gene 103910) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hirano, M. et al., Sci. Rep. 6:20989, 2016)
(Japanese text [open/close])
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14159 human MLC20 T18A DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14159_A6Epp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14159_A6Epa.seq
>D03455A2_A6Ep_pAxCA_F1_A12_03.ab1
    1 GACCGGGGCA ACCATGTTCA GCCTTCTTCT TTTTCCTAAC AGCTCCTGGG CAACGTGCTG
   61 GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGATTT ATCGATTTGA TCCCCACCAT
  121 GTCGAGCAAA AAGGCAAAGA CCAAGACCAC CAAGAAGCGC CCTCAGCGTG CAGCATCCAA
  181 TGTGTTTGCC ATGTTTGACC AGTCACAGAT TCAGGAGTTC AAAGAGGCCT TCAACATGAT
  241 TGATCAGAAC AGAGATGGCT TCATCGACAA GGAAGATTTG CATGATATGC TTGCTTCTCT
  301 AGGGAAGAAT CCCACTGATG CATACCTTGA TGCCATGATG AATGAGGCCC CAGGGCCCAT
  361 CAATTTCACC ATGTTCCTGA CCATGTTTGG TGAGAAGTTA AATGGCACAG ATCCTGAAGA
  421 TGTCATCAGA AACGCCTTTG CTTGCTTTGA TGAAGAAGCA ACAGGCACCA TTCAGGAAGA
  481 TTACCTAAGA GAGCTGCTGA CAACCATGGG GGATCGGTTT ACAGATGAGG AAGTGGATGA
  541 GCTGTACAGA GAAGCACCTA TTGACAAAAA GGGGAATTTC AATTACATCG AAGTTCACAC
  601 GCATCCTGAA ACATGGAGCC AAAGACAAAG ATGACTGAAA AGAACTTTAG CTAAAAGGGC
  661 CCGCGAAATT AATTAAAACT AGTCTAGAAA TCGATAATCA ATTCACTCCT CAGGTGCAAG
  721 GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCCAATGCC CCTGGCTCAC AAAATACCAC
  781 TGAGATCTTT TTTCCCTCTG CCAAAAAATT ATGGGGGACA TCATGAAAGC CCCCTTTGAG
  841 CATCTGACTT TCTGGCTAAT AAAAGGAAAA TTTATTTTTT CATTGGCAAT AGTGTGTTGG
  901 GAATTTTTTT GTGTCCTCTC ACCTCGGAAG GGACATATGG GGAAGGGCAA ATCAATTTAA
  961 AAACATCAGA ATGAGTATTT TGGTTTAGAG GTTTGGCCAA CATTATGCCC CAAATGCTTG
 1021 GCCTGCCATG AACAAAAGCT TGGCTATTAA AAGAGAGTCA ATCAGATATA TATGAAACAA
 1081 CCCCCTCATG CTGGTCCACT CCGTATTTCC ATAGAAAAGC CCTGGATAAG AAAGTTTTTT
1141 ATTTTGTATG TAATTTCTAA TT
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

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References

original Hirano, M., Myosin di-phosphorylation and peripheral actin bundle formation as initial events during endothelial barrier disruption. Sci. Rep. 6: 20989 (2016). PMID 26863988.

2017.10.19

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