Resource data sheet
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pIDT-SMART(C-TSC)

Expression vector with the improved high expression cassette C-TSC.

Catalog number RDB14136
Resource name pIDT-SMART(C-TSC)
Clone info. Expression vector with the improved high expression cassette C-TSC. Rerer Fig.3 of Sakaguchi, M., Mol. Biotechnol., 56, 621-630, 2014 (PMID: 24526517).
Corresponding gene/region: human TERT 1618 to1806 of DQ26472,0.2 kb; simian virus 40 promoter 2156 to 2474 of AY864928, 0.3 kb; cytomegalovirus promoter 159 to 637 of AJ318513, 0.5 kb; HTLV-11 RU5' of J02029, 0.3 kb; respectively.
Vector backbone pIDT-SMART (Plasmid)
Size of vector backbone 2.0 kb
Selectable markers Kan^r (E. coli).
Depositor Sakaguchi, Masakiyo |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)
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RDB14136 pIDT-SMART(C-TSC) DNA solution

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References

original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14136_A6Dmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: BGH_rev2 RDB14136_A6Dma.seq
>D03160A6_A6Dm_1_BGH_rev2_C02_08.ab1
    1 CTATCATGTT AGTAAGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGT TAGTCATCTA GCTCTAGAGC AAAAGGCCTT
  121 TTCCGCTCGA GCGGCGCGGG AGGCTGCTGG TTTTCCACTA CCCGAAAAAA ATCCAGCGTC
  181 TAAGCAGCTG CAAGGAGAGC CTTTCAGAGA AGCGGGTCCT GGCAGCGGCG GGGAAGTGTC
  241 CCCAAATGGG CAGAATAGCC TCCCCGCGTC GGGAGAGTCG CGTCCTTGCT CGGGTGTTGT
  301 AAGTTCCAGT GCAAAGTGCC CGCCCGCTGC TATGGGCAAA GTTTCGTGGA TGCGGCAAGG
  361 GTTGCGGACC GCTGGCTGGG GGATCAGCGG GAGGGCTGGG CCAGAGGCGA AGCCCCCTAT
  421 TCGCTCCGGA TCTCCCTTCC CAGGACGCCC GCAGCGCAGC TCTGCTCGCC CGGCTCTTCC
  481 ACCCTAGCCG GCCGCCCGCT CGCTCCCTCT GCCTCTCGCT GGAATTAGGG GCTAGCTAAC
  541 CCAAGCTTGG GCGCGGATCC GCGAGGATCG CGGCCACCAG GGAGAGCTTG ATCTTGCCAG
  601 CCAGTAGGGC AGCAGCAGGC AGTCCAGCCA CAGGCCGTAG CACAGGAGCA CAGCGCAATT
  661 TCGACTTGCA GGCGGCAGGG GCCCGGGGTC ACAAGGCGCC ACGAACCAGG CGAAGGGCAG
  721 GTCTAGTAAT ACAGGCCGCG GCGCTTCCCT CTCACACTCG CGAAACACCC CAATAGGTCA
  781 ATCTGTCTGT GCTGTCTTGG CCGGCGTCGA ACTAGTCCTC GGGGGTACCC CGGGAAGATC
  841 TTCCCCGGAA TTCCGGGGTG GCTTGGATCT GTAACGGCGC AGAACAGAAA ACGAAACAAA
  901 GACGTAGAGT TGAGCAAGCA GGGTCAGGCA AAGCGTGGAG AGCCGGCTGA GTCTAGGTAG
  961 GCTCCAAAGG GAGCGCCCGG ACAAAGGCCC GGGTCTCGAC CTGAGCTTTA AACTTTACCT
 1021 TAGACGGCGG ACGCAGTTTC AGAGGCACCA CAGCGGGAGC GCAGAAACGC GACTCATCAG
1081 CGTGGATGGC GGCCTTCAGG ATAAGGCCGG GCCTG
//
Primer: M13fwd RDB14136_A6Dmb.seq
>D03160A6_A6Dm_1_M13fwd_C11_08.ab1
    1 CAAAACAGCT GATCGTGGAC GGAGGTGAGC CAGTGAGTTG ATTGCAGTCC AGTTACGCTG
   61 GAGTCTGAGG CTCGTCCTGA ATGATACGCG TCGGAGGGTT GCGTTTGAGA CGGGCGACAG
  121 ATCGACACTG CTCGATCCGC TCGCACCACG CGTCGACGTC GGCCATAAAT TTTTTGCAAA
  181 AGCCTTGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG
  241 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCTTG GGGCGGAGAA ACTATCGTTG
  301 CTGACTAATT GAGATCGGAG TACTGTCCTC CGCGTTACAT AACTTACGGT AAATGGCCCG
  361 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
  421 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
  481 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
  541 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
  601 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC
  661 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC
  721 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC
  781 GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT
  841 CGTTTTAGTG AACCGTCAGA TCGCCTGGGA GACGCCATCC ACGCTGTTTT TGACCTCCAT
  901 AGAAGACACC GGGGACCGAT CCAGCCTTCC GCGGCCGGGG AAACGGTTGC ATTTGGGAAC
  961 GCGGATTCCC CCGTTGCCAA GAGTTGACGT AAGTACCGCC TTATAGACTC TATAGCACAC
 1021 CCCCTTTTGG CTCTTAATCA ATCCACTTAA TTACGACTCA CTATAAGGCT AGACCAGGAC
1081 CTGATTCTCC CTTT
//
Primer: RU5-R RDB14136_A6Dmc.seq
>D03160A6_A6Dm_1_RU5-R_D05_11.ab1
    1 ATCGTGAGCA GCAGGTCAGG CAAGCGTGGA GAGCCGGCTG AGTCTAGGTA GGCTCCAAGG
   61 GAGCGCCGGA CAAAGGCCCG GTCTCGACCT GAGCTTTAAA CTTACCTAGA CGGCGGACGC
  121 AGTTCAGGAG GCACCACAGG CGGGAGGCGG CAGAACGCGA CTCAACCGGC GTGGATGGCG
  181 GCCTCAGGTA GGGCGGCGGG CGCGTGAAGG AGAGATGCGA GCCCCTCGAA GCCAGAAAAG
  241 ACCCAGGAAA GGAACAGTCT GTCTCCCTAT AGTGAGTCGT ATTAATTGAT GGATAAGAGC
  301 CAAAGGGGTG TGCCTATAGA GTCTATAGGC GGTACTTACG TCACTCTTGG CACGGGGAAT
  361 CCGCGTTCCA ATGCACCGTT CCCGGCCGCG GAGGCTGGAT CGGTCCCGGT GTCTTCTATG
  421 GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA GGCGATCTGA CGGTTCACTA AACGAGCTCT
  481 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
  541 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
  601 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
  661 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
  721 AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT
  781 GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT
  841 TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG
  901 AAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGGTCA GCCAGGCCGG
  961 GCCCATTTAC CGTAAGGTTA TGTACGCGGG AGGACAGTAC TCCGAATCTC ATTTAGTCAG
 1021 CACGATAGGT TTCTCCGGCC CCAAGGCTGA ACTAATTTTT TTTAGGTATG GCAGGAGGCC
1081 CGAAGGGT
//
Primer: BGH_F2 RDB14136_A6Dmd.seq
>D03160A6_A6Dm_1_BGH_F2_C05_08.ab1
    1 TTTGCATCTG TGCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA
   61 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
  121 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG
  181 GCTCTATGGC GGAGTACTGT CCTCCGCTTC CCACGTGGCG GAGGGACTGG GGACCCGGGC
  241 ACCCGTCCTG CCCCTTCACC TTCCAGCTCC GCCTCCTCCG CGCGGACCCC GCCCCGTCCC
  301 GACCCCTCCC GGGTCCCCGG CCCAGCCCCC TCCGGGCCCT CCCAGCCCCT CCCCTTCCTT
  361 TCCGCGGCCC CGCCCTCTCC TCGCGGCGCG AGTTTTGGAA AGTCCCCAGG CTCCCCAGCA
  421 GGCAGAAGTA TCCAAAGCAT CCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
  481 GGCTCCCCAG CAGGCAGAAG TATCCAAAGC ATCCATCTCA ATTAGTCAGC AACCATAGTC
  541 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
  601 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA
  661 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCAAGGCT TTTGCAAAAA GCTCCGTTAC
  721 ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC
  781 AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT
  841 GGAGTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC
  901 GCCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCCT GGCATTATGC CCAGTACATG
  961 ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TTAGTCATCG CTATTAACAT
 1021 GGGTGATGCG AATTTGCCAG TACCATCCAT GAGCGATGAT TAGCCGAATT TGACTCACGG
1081 GGAAAT
//
Primer: M13R RDB14136_A6Dme.seq
>D03160A6_A6Dm_1_M13R_D11_11.ab1
    1 CTAAAACAAA GCTGATCGTG GACCGATCAT ACGTATAATG CCGTAAGATC GCGGCCGCCG
   61 GACGACAGCT CGCTCGTCCA GAACTGATCC AGTCGCGCTG CTCTCGTCGA TCCTTGGCGC
  121 GCCAACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC ATTTTGGAAA
  181 GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT GGAGACTTGG
  241 AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA ACCGCATCAC
  301 CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT CCCATAAGGT
  361 CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA TAGGGGGCGT
  421 ACTTGGCATA TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA AATACTCCAC
  481 CCATTGACGT CAATGGAAAG TCCCTATTGG CGTTACTATG GGAACATACG TCATTATTGA
  541 CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG TAAGTTATGT
  601 AACGGAGCTT TTTGCAAAAG CCTTGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA
  661 GCTCAGAGGC AGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG
  721 GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGGCGGG
781 ACTATGGTTG CTGACTAATT GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl