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pIDT-SMART(C-TSC) (#RDB14136)

Expression vector with the improved high expression cassette C-TSC.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sakaguchi, M., Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector with the improved high expression cassette C-TSC. Rerer Fig.3 of Sakaguchi, M., Mol. Biotechnol., 56, 621-630, 2014 (PMID: 24526517).
Comment Corresponding gene/region: human TERT 1618 to1806 of DQ26472,0.2 kb; simian virus 40 promoter 2156 to 2474 of AY864928, 0.3 kb; cytomegalovirus promoter 159 to 637 of AJ318513, 0.5 kb; HTLV-11 RU5' of J02029, 0.3 kb; respectively.
Vector backbone pIDT-SMART (Plasmid)
Size of vector backbone 2.0 kb
Selectable markers Kan^r (E. coli).
Growth conditions 37oC, LB + Kan
Depositor|Developer Sakaguchi, Masakiyo |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)。

Catalog # Resource name Shipping form Fee
RDB14136 pIDT-SMART(C-TSC) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pIDT-SMART(C-TSC) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14136).

Reference section:

Sakaguchi, M., Watanabe, M., Kinoshita, R., Kaku, H., Ueki, H., Futami, J., Murata, H., Inoue, Y., Li, S.A., Huang, P., Putranto, E.W., Ruma, I.M., Nasu, Y., Kumon, H., Huh, N.H., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517. [PubMed] [Article] [RRC of NBRP]