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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pIDT-SMART(C-TSC) (#RDB14136)

Expression vector with the improved high expression cassette C-TSC.

Clone info. Expression vector with the improved high expression cassette C-TSC. Rerer Fig.3 of Sakaguchi, M., Mol. Biotechnol., 56, 621-630, 2014 (PMID: 24526517).
Comment Corresponding gene/region: human TERT 1618 to1806 of DQ26472,0.2 kb; simian virus 40 promoter 2156 to 2474 of AY864928, 0.3 kb; cytomegalovirus promoter 159 to 637 of AJ318513, 0.5 kb; HTLV-11 RU5' of J02029, 0.3 kb; respectively.
Vector backbone pIDT-SMART (Plasmid)
Size of vector backbone 2.0 kb
Selectable markers Kan^r (E. coli).
Depositor|Developer Sakaguchi, Masakiyo |
 
Sequence (full) RDB14136zzt01.seq compiled by us

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)。

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RDB14136 pIDT-SMART(C-TSC) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pIDT-SMART(C-TSC) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14136).

Reference section:

Sakaguchi, M., Watanabe, M., Kinoshita, R., Kaku, H., Ueki, H., Futami, J., Murata, H., Inoue, Y., Li, S.A., Huang, P., Putranto, E.W., Ruma, I.M., Nasu, Y., Kumon, H., Huh, N.H., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14136_A6Dmp1-4.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: BGH_rev2 (Pr0606)
Region: bGH pA
Sequence file: RDB14136_A6Dmk.seq check
>D03160A6_A6Dm_1_BGH_rev2_C02_08.ab1
    1 NNNNNNNNNN NNNNNNGNNN GGNNGNNGGG NAACAACAGA TGGCTGGCAA CTAGAAGGCA
   61 CAGTCGAGGC TGATCAGCGG GTTTAAACGT TAGTCATCTA GCTCTAGAGC AAAAGGCCTT
  121 TTCCGCTCGA GCGGCGCGGG AGGCTGCTGG TTTTCCACTA CCCGAAAAAA ATCCAGCGTC
  181 TAAGCAGCTG CAAGGAGAGC CTTTCAGAGA AGCGGGTCCT GGCAGCGGCG GGGAAGTGTC
  241 CCCAAATGGG CAGAATAGCC TCCCCGCGTC GGGAGAGTCG CGTCCTTGCT CGGGTGTTGT
  301 AAGTTCCAGT GCAAAGTGCC CGCCCGCTGC TATGGGCAAA GTTTCGTGGA TGCGGCAAGG
  361 GTTGCGGACC GCTGGCTGGG GGATCAGCGG GAGGGCTGGG CCAGAGGCGA AGCCCCCTAT
  421 TCGCTCCGGA TCTCCCTTCC CAGGACGCCC GCAGCGCAGC TCTGCTCGCC CGGCTCTTCC
  481 ACCCTAGCCG GCCGCCCGCT CGCTCCCTCT GCCTCTCGCT GGAATTAGGG GCTAGCTAAC
  541 CCAAGCTTGG GCGCGGATCC GCGAGGATCG CGGCCACCAG GGAGAGCTTG ATCTTGCCAG
  601 CCAGTAGGGC AGCAGCAGGC AGTCCAGCCA CAGGCCGTAG CACAGGAGCA CAGCGCAATT
  661 TCGACTTGCA GGCGGCAGGG GCCCGGGGTC ACAAGGCGCC ACGAACCAGG CGAANGGCAG
  721 GTCTAGTAAT ACAGGCCGCG GCGCTTCCCT CTCACACTCG CGNAACACCC CAATAGGTCA
  781 ATCTGTCTGT GCTGTCTTGN NCGGCGTCGA ACTAGTCCTC GGGGGTACCC CNGGAAGATC
  841 TTCNNCGGAA TTCCGGGGTG NCTNGGATCT GTANCGGCGC AGANCAGAAA ANGAAACAAA
  901 GACGTAGANT TGANCAAGCN NGNTCNNNNA AAGCGTGGAG ANCNNGCNNA NNNTNNNTAN
961 GNNCCNANNG NNNCNNCNNN NNNANNNNCN NNNNNNNNN
//
Primer: M13fwd (Pr0349)
Region: CMV 1(CMV en,CMV pro)
Sequence file: RDB14136_A6Dml.seq check
>D03160A6_A6Dm_1_M13fwd_C11_08.ab1
    1 NNNNNNNNNN NNNNNNGNNN NNNNGNGNNC CNGTGNGTTG NNTGCAGTCC AGTTACGCTG
   61 GANTCTGAGG CTCGTCCTGA ATGATACGCG TCGGAGGGTT GCGTTTGAGA CGGGCGACAG
  121 ATCGACACTG CTCGATCCGC TCGCACCACG CGTCGACGTC GGCCATAAAT TTTTTGCAAA
  181 AGCCTTGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG
  241 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCTTG GGGCGGAGAA ACTATCGTTG
  301 CTGACTAATT GAGATCGGAG TACTGTCCTC CGCGTTACAT AACTTACGGT AAATGGCCCG
  361 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
  421 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
  481 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
  541 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
  601 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC
  661 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC
  721 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CNGGACTTTC CAAAATGTCG TANCAACTCC
  781 GCCCCATTGA CGCAAATGGG CNGTANGCGT GTACNGTGGG GANGTCTATA TAAGCAGANC
  841 TCGNTTNNGT GAACCGTCAG NTCGCCNGGN ANNNGNNNTC CACNCNNNTT TGANNNNNNN
901 ANNNNANNNN NNNNNN
//
Primer: RU5-R (Pr0384)
Region: RU5',CMV 1(CMV pro,CMV en)
Sequence file: RDB14136_A6Dmm.seq check
>D03160A6_A6Dm_1_RU5-R_D05_11.ab1
    1 NNNNNNNNCA NCNNNNCNGG CNAGCGTGGN NAGCCGGCTG NNTCTAGGTA GGCTCCAAGG
   61 GAGCGCCGGA CAAAGGCCCG GTCTCGACCT GAGCTTTAAA CTTACCTAGA CGGCGGACGC
  121 AGTTCAGGAG GCACCACAGG CGGGNGGCGG CAGAACGCGA CTCAACCGGC GTGGATGGCG
  181 GCCTCAGGTA GGGCGGCGGG CGCGTGAAGG AGAGATGCGA GCCCCTCGAA GCCAGAAAAG
  241 ACCCAGGAAA GGAACAGTCT GTCTCCCTAT AGTGAGTCGT ATTAATTGAT GGATAAGAGC
  301 CAAAGGGGTG TGCCTATAGA GTCTATAGGC GGTACTTACG TCACTCTTGG CACGGGGAAT
  361 CCGCGTTCCA ATGCACCGTT CCCGGCCGCG GAGGCTGGAT CGGTCCCGGT GTCTTCTATG
  421 GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA GGCGATCTGA CGGTTCACTA AACGAGCTCT
  481 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
  541 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
  601 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
  661 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
  721 ANGAAAGTCC CATAANGTCA TGTACTNNNC ATAATGCCAG GCGGGCCATT TACCGTCATT
  781 GACGTCNATA NGGGGCGTAC TTGNNATATG ATACANTTGA TGNACTGCCA ANNNGGCAGT
  841 TTANCGNAAA TACTCCANCN NNNNNCGNNN ANNGNAANNN CNNATNNNNG NNANNNNNGG
901 NANNNTACGN CATNNNNNNN NNN
//
Primer: BGH_F2 (Pr0385)
Region: bGHp(A),hTERT,SV40
Sequence file: RDB14136_A6Dmn.seq check
>D03160A6_A6Dm_1_BGH_F2_C05_08.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNCNTGGNNN GNGCCNCTCC CNCTGTCCTT TCCTAATAAA
   61 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
  121 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG
  181 GCTCTATGGC GGAGTACTGT CCTCCGCTTC CCACGTGGCG GAGGGACTGG GGACCCGGGC
  241 ACCCGTCCTG CCCCTTCACC TTCCAGCTCC GCCTCCTCCG CGCGGACCCC GCCCCGTCCC
  301 GACCCCTCCC GGGTCCCCGG CCCAGCCCCC TCCGGGCCCT CCCAGCCCCT CCCCTTCCTT
  361 TCCGCGGCCC CGCCCTCTCC TCGCGGCGCG AGTTTTGGAA AGTCCCCAGG CTCCCCAGCA
  421 GGCAGAAGTA TCCAAAGCAT CCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
  481 GGCTCCCCAG CAGGCAGAAG TATCCAAAGC ATCCATCTCA ATTAGTCAGC AACCATAGTC
  541 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
  601 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGANG CCGCCTCTGC CTCTGAGCTA
  661 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCAAGGCT TTTGCAAAAA GCTCCGTTAC
  721 ATAACTTACG GTAAATGGCC CGCCTGGCTG ANCGCCCAAC GACCCCCGCC CATTGACGTC
  781 AATAATGACG TATGTTCCCA TANNAACGCC NATANGGNNT TTCCATTNAC GTCAATGNGT
  841 GNANTANTTA CNGNAAANNG CCCANTNNGC ANNACATCNN NNGTATCATA NGCNAANNAC
901 NNCCCCCNAN NNNNNNNNN
//
Primer: M13R (Pr0160)
Region: CMV 2(CMV en),SV40
Sequence file: RDB14136_A6Dmo.seq check
>D03160A6_A6Dm_1_M13R_D11_11.ab1
    1 NNNNNNNNNN NNNNNNNNGG NNNNNTCNTA CGTATAATGC CGTANGNNCG CGGCCGCCGG
   61 NNGACAGCTC GCTCGTCCAG AACTGATCCA GTCGCGCTGC TCTCGTCGAT CCTTGGCGCG
  121 CCAACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG
  181 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA
  241 AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC
  301 ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC
  361 ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA
  421 CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC
  481 CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC
  541 GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA
  601 ACGGAGCTTT TTGCAAAAGC CTTGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG
  661 CTCAGANGCA GANGCGGCCT CNGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG
  721 CGGANAATGG GCGGAACTGG GCGGANTTAN GGGCGGGATG GGCGGANTTA GGGGCGGGAC
  781 TATGGTTGCT GACTAATTGA GATGGATGCT TTGGATACTT CTGCCTGCTG GGGANCCNGG
  841 GGACTTTCCA CACCTGGTTG CTGACNNANN TGAGATNGAT GCTTTNNATA CTTCNNNNNN
  901 CNGGGGNNNC NTGGGGNNNT TTNNNNAAAC TCGCGCCNCN NNGNNNANGG NNGGGNNNNN
961 NNNNANNNNN NNNGNNNNGN NNNGNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517. [link to RRC of NBRP]