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pIDT-SMART(C-TSC)

Expression vector with the improved high expression cassette C-TSC.

Catalog number RDB14136
Resource name pIDT-SMART(C-TSC)
Clone info. Expression vector with the improved high expression cassette C-TSC. Rerer Fig.3 of Sakaguchi, M., Mol. Biotechnol., 56, 621-630, 2014 (PMID: 24526517).
Comment Corresponding gene/region: human TERT 1618 to1806 of DQ26472,0.2 kb; simian virus 40 promoter 2156 to 2474 of AY864928, 0.3 kb; cytomegalovirus promoter 159 to 637 of AJ318513, 0.5 kb; HTLV-11 RU5' of J02029, 0.3 kb; respectively.
Vector backbone pIDT-SMART (Plasmid)
Size of vector backbone 2.0 kb
Selectable markers Kan^r (E. coli).
Depositor|Developer Sakaguchi, Masakiyo |
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            Reference sequence
              

            Distribution information

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            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)。
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            RDB14136 pIDT-SMART(C-TSC) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB14136zzt01.seq compiled by us

            Original reference

            original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14136_A6Dmp1-4.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: BGH_rev2
            Region: bGH pA
            Sequence file: RDB14136_A6Dmk.seq check
            >D03160A6_A6Dm_1_BGH_rev2_C02_08.ab1
                1 NNNNNNNNNN NNNNNNGNNN GGNNGNNGGG NAACAACAGA TGGCTGGCAA CTAGAAGGCA
               61 CAGTCGAGGC TGATCAGCGG GTTTAAACGT TAGTCATCTA GCTCTAGAGC AAAAGGCCTT
              121 TTCCGCTCGA GCGGCGCGGG AGGCTGCTGG TTTTCCACTA CCCGAAAAAA ATCCAGCGTC
              181 TAAGCAGCTG CAAGGAGAGC CTTTCAGAGA AGCGGGTCCT GGCAGCGGCG GGGAAGTGTC
              241 CCCAAATGGG CAGAATAGCC TCCCCGCGTC GGGAGAGTCG CGTCCTTGCT CGGGTGTTGT
              301 AAGTTCCAGT GCAAAGTGCC CGCCCGCTGC TATGGGCAAA GTTTCGTGGA TGCGGCAAGG
              361 GTTGCGGACC GCTGGCTGGG GGATCAGCGG GAGGGCTGGG CCAGAGGCGA AGCCCCCTAT
              421 TCGCTCCGGA TCTCCCTTCC CAGGACGCCC GCAGCGCAGC TCTGCTCGCC CGGCTCTTCC
              481 ACCCTAGCCG GCCGCCCGCT CGCTCCCTCT GCCTCTCGCT GGAATTAGGG GCTAGCTAAC
              541 CCAAGCTTGG GCGCGGATCC GCGAGGATCG CGGCCACCAG GGAGAGCTTG ATCTTGCCAG
              601 CCAGTAGGGC AGCAGCAGGC AGTCCAGCCA CAGGCCGTAG CACAGGAGCA CAGCGCAATT
              661 TCGACTTGCA GGCGGCAGGG GCCCGGGGTC ACAAGGCGCC ACGAACCAGG CGAANGGCAG
              721 GTCTAGTAAT ACAGGCCGCG GCGCTTCCCT CTCACACTCG CGNAACACCC CAATAGGTCA
              781 ATCTGTCTGT GCTGTCTTGN NCGGCGTCGA ACTAGTCCTC GGGGGTACCC CNGGAAGATC
              841 TTCNNCGGAA TTCCGGGGTG NCTNGGATCT GTANCGGCGC AGANCAGAAA ANGAAACAAA
              901 GACGTAGANT TGANCAAGCN NGNTCNNNNA AAGCGTGGAG ANCNNGCNNA NNNTNNNTAN
            961 GNNCCNANNG NNNCNNCNNN NNNANNNNCN NNNNNNNNN
            //
            Primer: M13fwd
            Region: CMV 1(CMV en,CMV pro)
            Sequence file: RDB14136_A6Dml.seq check
            >D03160A6_A6Dm_1_M13fwd_C11_08.ab1
                1 NNNNNNNNNN NNNNNNGNNN NNNNGNGNNC CNGTGNGTTG NNTGCAGTCC AGTTACGCTG
               61 GANTCTGAGG CTCGTCCTGA ATGATACGCG TCGGAGGGTT GCGTTTGAGA CGGGCGACAG
              121 ATCGACACTG CTCGATCCGC TCGCACCACG CGTCGACGTC GGCCATAAAT TTTTTGCAAA
              181 AGCCTTGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG
              241 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCTTG GGGCGGAGAA ACTATCGTTG
              301 CTGACTAATT GAGATCGGAG TACTGTCCTC CGCGTTACAT AACTTACGGT AAATGGCCCG
              361 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
              421 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
              481 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
              541 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
              601 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC
              661 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC
              721 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CNGGACTTTC CAAAATGTCG TANCAACTCC
              781 GCCCCATTGA CGCAAATGGG CNGTANGCGT GTACNGTGGG GANGTCTATA TAAGCAGANC
              841 TCGNTTNNGT GAACCGTCAG NTCGCCNGGN ANNNGNNNTC CACNCNNNTT TGANNNNNNN
            901 ANNNNANNNN NNNNNN
            //
            Primer: RU5-R
            Region: RU5',CMV 1(CMV pro,CMV en)
            Sequence file: RDB14136_A6Dmm.seq check
            >D03160A6_A6Dm_1_RU5-R_D05_11.ab1
                1 NNNNNNNNCA NCNNNNCNGG CNAGCGTGGN NAGCCGGCTG NNTCTAGGTA GGCTCCAAGG
               61 GAGCGCCGGA CAAAGGCCCG GTCTCGACCT GAGCTTTAAA CTTACCTAGA CGGCGGACGC
              121 AGTTCAGGAG GCACCACAGG CGGGNGGCGG CAGAACGCGA CTCAACCGGC GTGGATGGCG
              181 GCCTCAGGTA GGGCGGCGGG CGCGTGAAGG AGAGATGCGA GCCCCTCGAA GCCAGAAAAG
              241 ACCCAGGAAA GGAACAGTCT GTCTCCCTAT AGTGAGTCGT ATTAATTGAT GGATAAGAGC
              301 CAAAGGGGTG TGCCTATAGA GTCTATAGGC GGTACTTACG TCACTCTTGG CACGGGGAAT
              361 CCGCGTTCCA ATGCACCGTT CCCGGCCGCG GAGGCTGGAT CGGTCCCGGT GTCTTCTATG
              421 GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA GGCGATCTGA CGGTTCACTA AACGAGCTCT
              481 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
              541 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
              601 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
              661 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
              721 ANGAAAGTCC CATAANGTCA TGTACTNNNC ATAATGCCAG GCGGGCCATT TACCGTCATT
              781 GACGTCNATA NGGGGCGTAC TTGNNATATG ATACANTTGA TGNACTGCCA ANNNGGCAGT
              841 TTANCGNAAA TACTCCANCN NNNNNCGNNN ANNGNAANNN CNNATNNNNG NNANNNNNGG
            901 NANNNTACGN CATNNNNNNN NNN
            //
            Primer: BGH_F2
            Region: bGHp(A),hTERT,SV40
            Sequence file: RDB14136_A6Dmn.seq check
            >D03160A6_A6Dm_1_BGH_F2_C05_08.ab1
                1 NNNNNNNNNN NNNNNNNTTG NNCNTGGNNN GNGCCNCTCC CNCTGTCCTT TCCTAATAAA
               61 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
              121 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG
              181 GCTCTATGGC GGAGTACTGT CCTCCGCTTC CCACGTGGCG GAGGGACTGG GGACCCGGGC
              241 ACCCGTCCTG CCCCTTCACC TTCCAGCTCC GCCTCCTCCG CGCGGACCCC GCCCCGTCCC
              301 GACCCCTCCC GGGTCCCCGG CCCAGCCCCC TCCGGGCCCT CCCAGCCCCT CCCCTTCCTT
              361 TCCGCGGCCC CGCCCTCTCC TCGCGGCGCG AGTTTTGGAA AGTCCCCAGG CTCCCCAGCA
              421 GGCAGAAGTA TCCAAAGCAT CCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
              481 GGCTCCCCAG CAGGCAGAAG TATCCAAAGC ATCCATCTCA ATTAGTCAGC AACCATAGTC
              541 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
              601 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGANG CCGCCTCTGC CTCTGAGCTA
              661 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCAAGGCT TTTGCAAAAA GCTCCGTTAC
              721 ATAACTTACG GTAAATGGCC CGCCTGGCTG ANCGCCCAAC GACCCCCGCC CATTGACGTC
              781 AATAATGACG TATGTTCCCA TANNAACGCC NATANGGNNT TTCCATTNAC GTCAATGNGT
              841 GNANTANTTA CNGNAAANNG CCCANTNNGC ANNACATCNN NNGTATCATA NGCNAANNAC
            901 NNCCCCCNAN NNNNNNNNN
            //
            Primer: M13R
            Region: CMV 2(CMV en),SV40
            Sequence file: RDB14136_A6Dmo.seq check
            >D03160A6_A6Dm_1_M13R_D11_11.ab1
                1 NNNNNNNNNN NNNNNNNNGG NNNNNTCNTA CGTATAATGC CGTANGNNCG CGGCCGCCGG
               61 NNGACAGCTC GCTCGTCCAG AACTGATCCA GTCGCGCTGC TCTCGTCGAT CCTTGGCGCG
              121 CCAACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG
              181 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA
              241 AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC
              301 ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC
              361 ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA
              421 CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC
              481 CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC
              541 GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA
              601 ACGGAGCTTT TTGCAAAAGC CTTGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG
              661 CTCAGANGCA GANGCGGCCT CNGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG
              721 CGGANAATGG GCGGAACTGG GCGGANTTAN GGGCGGGATG GGCGGANTTA GGGGCGGGAC
              781 TATGGTTGCT GACTAATTGA GATGGATGCT TTGGATACTT CTGCCTGCTG GGGANCCNGG
              841 GGACTTTCCA CACCTGGTTG CTGACNNANN TGAGATNGAT GCTTTNNATA CTTCNNNNNN
              901 CNGGGGNNNC NTGGGGNNNT TTNNNNAAAC TCGCGCCNCN NNGNNNANGG NNGGGNNNNN
            961 NNNNANNNNN NNNGNNNNGN NNNGNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Sakaguchi, M., Dramatic increase in expression of a transgene by insertion of promoters downstream of the cargo gene. Mol. Biotechnol. 56 (7): 621-30 (2014). PMID 24526517.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl