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pIDT-SMART(C-TSC)-hPINK1-HA(triple mutant) (#RDB14135)

Expression vector of C-terminal HA-tagged human PINK1, catalytically inactive KDD triple mutant (K219A/D362A/D384A).

Clone info. Expression vector of C-terminal HA-tagged human PINK1, catalytically inactive KDD triple mutant (K219A/D362A/D384A).
Comment cDNA fragment 95 to 1837 of NM_032409.2 (cds, 95-1840) is cloned. Known differences: C to T substitution at 283nt (Leu at 63aa, synonymous).
Vector backbone pIDT-SMART (C-TSC)Dep3160 (Plasmid)
Selectable markers Kan^r (E. coli).
Gene/insert name human PINK1 cDNA
Depositor|Developer Sakaguchi, Masakiyo | Murata, Hitoshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Murata, T. et al., PLoS One 10(11):e0142438, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Murata, T. et al., PLoS One 10(11):e0142438, 2015)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14135 pIDT-SMART(C-TSC)-hPINK1-HA(triple mutant) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pIDT-SMART(C-TSC)-hPINK1-HA(triple mutant) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14135).

Reference section:

Murata, H., Takamatsu, H., Liu, S., Kataoka, K., Huh, N.H., Sakaguchi, M., NRF2 Regulates PINK1 Expression under Oxidative Stress Conditions. PLoS One 10 (11): e0142438 (2015). PMID 26555609. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Murata, H., NRF2 Regulates PINK1 Expression under Oxidative Stress Conditions. PLoS One 10 (11): e0142438 (2015). PMID 26555609. [link to RRC of NBRP]