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pDll1-Fluc

Reporter vector for time-lapse imaging of Dll1 protein.

Catalog number RDB14128
Resource name pDll1-Fluc
Clone info. Reporter vector for time-lapse imaging of Dll1 protein. This construct contains Dll1 promoter, Dll1 gene and luciferase cDNA.
Expression was confirmed by the depositor with bioluminescence imaging. See Fig.S4 of Shimojo, H. et al., Genes Dev. 30 (1): 102-116, 2016.
Vector backbone pBluescript (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse Dll1 genomic DNA Mouse Dll1 genomic DNA firefly (Photinus pyralis) cDNA
Depositor Kageyama, Ryuichiro |
 
Other clones in our bank mouse Dll1 (NCBI Gene 13388) | mouse Dll1 (NCBI Gene 13388) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)
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RDB14128 pDll1-Fluc DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Notch signaling pathway (English text)

References

original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14128_A6Djp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB14128_A6Dja.seq
>D03343A2_A6Dj_1_M13_-40_A11_02.ab1
    1 TTTGCCGAAG GGAAATGGTA ATACGACTCA CTATAGGGCG AATTGGGGTA CCGGGGCCCC
   61 CCCTCGAGGT CGACGGTATC GATTGATACC AGGTGAGGTG AGAGCACTGT GGCCCGGGCC
  121 CAGCTGCATG GAACCAGCAC GACTTCGTCT CAAAGCTTTG TGACTCAAGT CGTACCCACA
  181 TCCACAGGCC CAGCGATGGC CACCTCTCTA GCACCGCCAC CGTCCAGAAG CCCCCAGGGG
  241 CGCTAACTCT AGTTGAGAGT CAGAAACGTA TGAGCAAACT AAACGCAACC CCTCCCCTGT
  301 TAGAGCCTTC TTCCCACAAA CCCCTCACCC CATTGCTACC CATTCCAGAC CAAAGGCCGC
  361 GGAAAGTTCC GAGCGAGGTG CACGCGTGAC TCTCTTCGGC TGGCCACGAG CAGCTGCTAG
  421 GATAATCTGA TCCTGGGACA AAGGCCCGCC CGCCCGGGCT GCAGCCGCAG GTAAACACGC
  481 GCTTTGTTAT GGAAATCAGC GCAGGACAGC CCGGAGCAGA CAACCCACGA TTCCGAGGAA
  541 GGAGAGGCAT AGGGGGAGGG GCCTCGGGTG GGGCGATCTG TCATCTGCGC GGCAGCCGCC
  601 AGCTGAGCCA CGCGCCTCCC CTTTTCTCAT GTAAATCAGC ACATGCCTTT GCAACTTTCT
  661 GCAGCCGGCT TCCCCAAGAC CCACGCACCC CAGGGTGAGG TGTCCTCGGA GCGCATACAA
  721 TGGCTCTGAG TTCCTATCTA GCTACCCAAA AGGAAAATCA CCAACTAGGT GGGTAGAAAG
  781 GAGGCGGCAG CCGGTTTCCA TCAAAGAAGA AAAGGGCTGT GATCCTACTG CATGACGGGA
  841 GTTCGGACCC CCGATCCACT CTGAAGTTTC ACGGGGCGCC CAATGCCGGA GTCTTTTGGG
  901 TTCATTACTC CCTTTTGAGG TGTACTGCCC CACAGAAACG CAGAAGGAGG GCTAGGCCCT
  961 ACCGGGTTTC CACCCTTGGT TGGGGTTGTG AATTCTTGGA AGGCTCGAAA GTCTTCTAAG
 1021 TCTCTAAATT GGAAGCCCAA ATTGCCTGAT TGCATTCAAA TGTGGTGCAC CAATGGTCTG
 1081 TGTACAAAAC ATTTAAAAGT GGTGTTGGCC GCCAGATTTA TTGGTGGCCA ATGCTGTAAA
1141 TTGTTAAAGT TCACAGGCAG GCGTTGAGGC GCAAGACGAG C
//
Primer: Luc_R RDB14128_A6Djb.seq
>D03343A2_A6Dj_1_Luc_R_G03_21.ab1
    1 ACATGGATTA AGGGAACAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG
   61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA
  121 CCTAGAAGGG CCCAGGAGAC AGGACAGGAA GTTAACTGCA GGCAGAAATG GACCCTCTCC
  181 ACATCACTCC CGTCCACGAC AAGAGGGCCG CACACTGACC TCAGTCGCTA TAACACACTC
  241 ATCCTTTTCT GCAGACAGAA CATACACCGA CTGGTACTTG GTGTCCTTTG AAGTAGAGTA
  301 GACAGACTCT GGCCTTTTTC TGTCAGGAAT CTCCCCACTG AAAGGAAAAT TAGAGAGAAC
  361 CACAGTAAAC ACACAGGAAA GTGGTTGAAC GCGGATATAC CCAGAACATG GACCGGTCTC
  421 CCTGCCCTCC TTGCCCAGCT CAGGGCACAT ACCCCCTAAG TGTTGGGGCG ATCTTCTCTT
  481 CTCCTGCAGA GCTCTGTGAC TGGCACTTGG TGTCACGTTT GCTGTGTGTA TCCCTGACCG
  541 TGGCTTCATC TCCCTTGAGG TCTCGAACGA GGTTATAGTC CACAGTGGGG TATCGGACCT
  601 TAAAGCTGCT CTTCTCGGCT CCATGGTCCC CGTGAAAGTC CGCCTTCTTG TTGGTGTTCT
  661 TGATCTGGGT AGCCCCAATG ATGCTAACAG AAACGTCCTT CTCGCGCTGG CAATTGGCTA
  721 GGTTGTTCAT GGTTTCTGTC TCTCCCCCAC AGGGTTCAGG TGGAGGCTGG TGTTTCTGTA
  781 GCTTCAGCCG GACGCAGACC ACCACAGCAG CACAGCCCAG CAGCAGCAGG AGGACAAGCA
  841 CCACCCCGGC ACACACGGCC ACCCAGGGGA AAGGGCCCGC CCTGGCTCTC CATATGCCTC
  901 TCACTGAGGT CCACCACCAT GGGCCCTGGT GGTGGCTCAG GGAGCAGAAA CTGGCAGTTT
  961 GGGGGCCCGC CATAGCCCCT GGGCGCACTC ACACATGTAG CGCTGGCCCC TCTGGGTGGG
 1021 CAGGTGGCCC CCAATTTAAT GGCAGGATGC ATTGCTCCAC ATCTTGCTGA CAGGGGCGCT
1081 GCAGATTCTT GCTCCGGATG TTAAGACCAA GAATGGGGGG A
//
Primer: Reverse2 RDB14128_A6Djc.seq
>D03343A2_A6Dj_1_Reverse2_B11_05.ab1
    1 GGGGCCAATG ATTACGGCCA AGCTCGGAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA
   61 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA
  121 ATCAAGCTTA TCGATTTGTA GTTCATTATA TTTATTTTGG AAAAATATTT TTAAAATGAA
  181 TTTCTTCATT AACAAAACAG TAAAAAACTC AAATAACATT TGCACAATAT TCCATAAATA
  241 CATTTATATA CAAAAAAATA TAGTCACATA GACCCGAAGT GCCTTTGTAC ATATTTACCA
  301 AAATTTAAAT TATAAAAAAA TGAACGTCAC AAAATACTCA AGCTTTACAG ATATCATGAA
  361 AAATATTTTT ACAAATCCCA AAAAAATAAC ACACGTTTTC TTTTTCCCGT CTAGGAAAAA
  421 AAAAAACACA TCTCAGTATC AAAAAGGACA ATAAAGGCAG TTGTGTTTCT AGTTCAAGGA
  481 AAGACTGGCT CATAGTAATC CAAGATAGAC GTGTGGGCAG TGCGTGCTTC CTATATGAAT
  541 CAAGTCACTC GTCCATTTAA ATATATACTC TTCAAAGCAA CTGTCCATAG TGCAATGGGA
  601 ACAACCAGCA GGCAGTCCAG CGGCAGCCAA AGCCTAGGCT GGCAGTGTCG GGCGCCTCTG
  661 CTAACTCTGA GAGAACCAGC TTCGCCTGAA CCTGGTTCTC AGCAGCAGTC CCTGGGTTTC
  721 CTCTCCCTTC CTCTCTCAGC AGCATTCATC GGGGCTATAT CCTTGGAATT TTATTTAAGA
  781 GAAATGGGGA ATTTTGCCAC ATCGCTTCCA TCTTACACGG CGATCTTTCC GCCCTTCTTG
  841 GCCTTTATGA GGATCTCTCT GATTTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTTCG
  901 GTACTTCGTC CACAAACACA ACTCCTCCGC GCAACTTTTT CGCGGGTTGT TACTTGACTG
  961 GGCGACGTAA TCCACGATCT CTTTTTTCCG TCATCGTCTT TCCGGTGCTC AAACAACACC
 1021 GCCGCGGGAG TTCACGGCGT TCATCGTCGG AAGACTGCGA CACTGCGTCG TAGATGATCG
1081 GTGTTGGGAG CCAAGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl