Resource data sheet
Prev.

pCAGIPuro-FlagmcMycWT

Expression vector of FLAG tagged mouse cMyc, wild type.

Catalog number RDB14100
Resource name pCAGIPuro-FlagmcMycWT
Clone info. Expression vector of FLAG tagged mouse cMyc, wild type.
Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Myc cDNA
Depositor Okazaki, Yasushi | Iseki, Hiroyoshi |
 
Other clones in our bank mouse Myc (NCBI Gene 17869) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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RDB14100 pCAGIPuro-FlagmcMycWT DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14100_A6Dcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB14100_A6Dca.seq
>D03255J1_A6Dc_pAxCA_F1_E06_15_ABI24.ab1
    1 GTAACGGTCT GCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCCT CGAGCCACCA TGGACTACAA
  121 GGACGACGAT GACAAGGGAG GAGGCATGCC CCTCAACGTG AACTTCACCA ACAGGAACTA
  181 TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC GACGAGGAAG AGAATTTCTA
  241 TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC AGTGAGGATA TCTGGAAGAA
  301 ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC CGCTCCGGGC TCTGCTCTCC
  361 ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA GACGATGACG GCGGCGGTGG
  421 CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG TTACTTGGAG GAGACATGGT
  481 GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC ATCAAGAACA TCATCATCCA
  541 GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG GTCTCGGAGA AGCTGGCCTC
  601 CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG
  661 CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC
  721 CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC
  781 CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG
  841 GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC
  901 TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC
  961 CCCTGCCAAG AGGTCGAGTC GGGCTCATCT CCATCCCGAG GCCACAGCAA AACCTCCGCC
 1021 ACAGCCCACT GGTCCCTCAG AGTGCAACGT CTCACTCACA GCACACTACG CGCACCCCTC
1081 CACAGGAATG ACTATCAGCT GGCAGAGGTA AGTGACAAGT TGGCCAGGAA TCCTGA
//
Primer: IRES-R RDB14100_A6Dcb.seq
>D03255J1_A6Dc_IRES-R_F06_18_ABI24.ab1
    1 GCTAGGCGGC TTCGGCCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGCGGAAT
   61 TCGATATCAA GCTTATCGAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC
  121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC
  181 TCGAGTTATG CACCAGAGTT TCGAAGCTGT TCGAGTTTGT GTTTCAATTG TTCTCGTCGT
  241 TTCCTCAATA AGTCCTTTTC AGAGGTGAGC TTGTGCTCGT CTGCTTGAAT GGACAGGATG
  301 TAGGCGGTGG CTTTTTTGAG GATCACTACC TTGGGGGCCT TTTCGTTGTT TTCCAATTCA
  361 GGGATCTGGT CACGCAGGGC AAAAAAGCTG CGCTTCAGCT CGTTCCTCCT CTGACGTTCC
  421 AAGACGTTGT GTGTCCGCCT CTTGTCGTTT TCCTCCGTGT CTGAGGACCT GGGGCTGGAG
  481 CACTTGCGGT TGTTGCTGAT CTGCTTCAGG ACCCTGCCAC TGTCCAACTT GGCCCTCTTG
  541 GCAGCTGGAT AGTCCTTCCT TGTGGAGGGG GGTGCGGCGT AGTTGTGCTG GTGAGTGGAG
  601 ACGTGGCACC TCTTGAGGAC CAGTGGGCTG TGCGGAGGTT TGCTGTGGCC TCGGGATGGA
  661 GATGAGCCCG ACTCCGACCT CTTGGCAGGG GTTTGCCTCT TCTCCACAGA CACCACATCA
  721 ATTTCTTCCT CATCTTCTTG CTCTTCTTCA GAGTCGCTGC TGGTGGTGGG CGGTGTCTCC
  781 TCATGCAGCA CTAGGGGCTC AGGCTGGCCC GTGGGGAGGA CTCGGAGGAC AGCAGCGAGT
  841 CCGAGGAAGG AGAGAAGGCC GTGGATCGGA CGAGTACAGG ATTTGGGCGA GCTGCTGTCG
  901 TGAGCGGTAG GGAAAGACCA CTGAGGGGTC ATGCACTCGA CGCGCGCGTG AGTCTGCAGT
  961 ACAGGCTGAG TGAGCGACGC TGTGCCGCGG CCGGGCTCAG CTGTGCTGCT TGCCGCAGCC
 1021 TGGTAGAAGC AGCTCCGGAC CAGCTTGCAC GGCTGAACGT CCATACAGTC TGGATGATGA
1081 GTTTCCTTGT GA
//
Primer: IRES-F RDB14100_A6Dcc.seq
>D03255J1_A6Dc_IRES-F_G06_21_ABI24.ab1
    1 GCAGTCGTTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGTGTC ATTCTATTCT GGGGGGGTGG GGGTGGGGCA GACAGCAAAG
  961 GGGAGGATTG GGAAGAACAA TTAGCAGGCA TGCTGGGGAT GCCGGTGGGC TCTATGGCTT
 1021 TCTTGGAAGG CGAAAGAATC TGCAGGCCCA GCTGGCCGTA TCATGTCATA GCTGATTCCT
1081 GGTTGAATGT TATCCGCTTC ACAAATTTCC CAACGAACAT AG
//
Primer: BGHpA_F RDB14100_A6Dcd.seq
>D03255J1_A6Dc_BGHpA_F_E09_05_ABI08.ab1
    1 CGGGAGGGTT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
   61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
  121 AACCTGCAGC CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
  181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
  241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
  301 AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
  421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
  481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTAACTTG TTTATTGCAG
  541 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  601 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCC
  661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
  721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
  781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG ATAACGCACG AAAGAACATG
  841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
  901 CCATAGGCTC CGCCCCCCCT GACGAGCATC ACAAAAAATC GACGCTCAAG TCAGAGTGGC
  961 GAAACCCGAC AGACTATAAA GATACCAGGC GTTTCCCCCC TGGAAGCTCC CCTCGTGCGC
 1021 CTCCTCCTGG TTCCGACCCT GGCGCTACCG AATACCTGGT CGCTTTCTCC TCCGAAGCGT
1081 GGCGCCTTTC CCTCCATAAG C
//
Primer: Amp_R RDB14100_A6Dce.seq
>D03255J1_A6Dc_Amp_R_A07_01_ABI24.ab1
    1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
   61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
  781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
  841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
  901 CGCCAATAGG ACTTTCCATT TGACGTCATG GGTGGAGTAT TTACGGTAAC TGCCCACTTG
  961 CAGTACATCC AGTGTATCAT ATGGCAGGTA CGCCCCCTAT TGACGTCAAT GAACGTTAAT
 1021 TGACCGGCTG GACATATGGC AAGTTACATT GACTATACAC TACTACTTGC AGTACATCTA
1081 CG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl