Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGIPuro-FlagmcMycWT (#RDB14100)

Expression vector of FLAG tagged mouse cMyc, wild type.

Clone info. Expression vector of FLAG tagged mouse cMyc, wild type.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Myc cDNA
Depositor|Developer Okazaki, Yasuji | Iseki, Hiroyoshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
付加的使用条件：
利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14100 pCAGIPuro-FlagmcMycWT DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIPuro-FlagmcMycWT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14100).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14100_A6Dcp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1
Sequence file: RDB14100_A6Dca.seq check
>D03255J1_A6Dc_pAxCA_F1_E06_15_ABI24.ab1
    1 GTAACGGTCT GCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
   61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCCT CGAGCCACCA TGGACTACAA
  121 GGACGACGAT GACAAGGGAG GAGGCATGCC CCTCAACGTG AACTTCACCA ACAGGAACTA
  181 TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC GACGAGGAAG AGAATTTCTA
  241 TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC AGTGAGGATA TCTGGAAGAA
  301 ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC CGCTCCGGGC TCTGCTCTCC
  361 ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA GACGATGACG GCGGCGGTGG
  421 CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG TTACTTGGAG GAGACATGGT
  481 GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC ATCAAGAACA TCATCATCCA
  541 GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG GTCTCGGAGA AGCTGGCCTC
  601 CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG
  661 CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC
  721 CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC
  781 CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG
  841 GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC
  901 TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC
  961 CCCTGCCAAG AGGTCGAGTC GGGCTCATCT CCATCCCGAG GCCACAGCAA AACCTCCGCC
 1021 ACAGCCCACT GGTCCCTCAG AGTGCAACGT CTCACTCACA GCACACTACG CGCACCCCTC
1081 CACAGGAATG ACTATCAGCT GGCAGAGGTA AGTGACAAGT TGGCCAGGAA TCCTGA
//
Primer: IRES-R
Sequence file: RDB14100_A6Dcb.seq check
>D03255J1_A6Dc_IRES-R_F06_18_ABI24.ab1
    1 GCTAGGCGGC TTCGGCCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGCGGAAT
   61 TCGATATCAA GCTTATCGAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC
  121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC
  181 TCGAGTTATG CACCAGAGTT TCGAAGCTGT TCGAGTTTGT GTTTCAATTG TTCTCGTCGT
  241 TTCCTCAATA AGTCCTTTTC AGAGGTGAGC TTGTGCTCGT CTGCTTGAAT GGACAGGATG
  301 TAGGCGGTGG CTTTTTTGAG GATCACTACC TTGGGGGCCT TTTCGTTGTT TTCCAATTCA
  361 GGGATCTGGT CACGCAGGGC AAAAAAGCTG CGCTTCAGCT CGTTCCTCCT CTGACGTTCC
  421 AAGACGTTGT GTGTCCGCCT CTTGTCGTTT TCCTCCGTGT CTGAGGACCT GGGGCTGGAG
  481 CACTTGCGGT TGTTGCTGAT CTGCTTCAGG ACCCTGCCAC TGTCCAACTT GGCCCTCTTG
  541 GCAGCTGGAT AGTCCTTCCT TGTGGAGGGG GGTGCGGCGT AGTTGTGCTG GTGAGTGGAG
  601 ACGTGGCACC TCTTGAGGAC CAGTGGGCTG TGCGGAGGTT TGCTGTGGCC TCGGGATGGA
  661 GATGAGCCCG ACTCCGACCT CTTGGCAGGG GTTTGCCTCT TCTCCACAGA CACCACATCA
  721 ATTTCTTCCT CATCTTCTTG CTCTTCTTCA GAGTCGCTGC TGGTGGTGGG CGGTGTCTCC
  781 TCATGCAGCA CTAGGGGCTC AGGCTGGCCC GTGGGGAGGA CTCGGAGGAC AGCAGCGAGT
  841 CCGAGGAAGG AGAGAAGGCC GTGGATCGGA CGAGTACAGG ATTTGGGCGA GCTGCTGTCG
  901 TGAGCGGTAG GGAAAGACCA CTGAGGGGTC ATGCACTCGA CGCGCGCGTG AGTCTGCAGT
  961 ACAGGCTGAG TGAGCGACGC TGTGCCGCGG CCGGGCTCAG CTGTGCTGCT TGCCGCAGCC
 1021 TGGTAGAAGC AGCTCCGGAC CAGCTTGCAC GGCTGAACGT CCATACAGTC TGGATGATGA
1081 GTTTCCTTGT GA
//
Primer: IRES-F
Sequence file: RDB14100_A6Dcc.seq check
>D03255J1_A6Dc_IRES-F_G06_21_ABI24.ab1
    1 GCAGTCGTTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
  121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
  181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
  241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
  301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
  361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
  421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
  481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
  541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
  601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
  661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
  721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
  781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  901 CGCATTGTCT GAGTAGTGTC ATTCTATTCT GGGGGGGTGG GGGTGGGGCA GACAGCAAAG
  961 GGGAGGATTG GGAAGAACAA TTAGCAGGCA TGCTGGGGAT GCCGGTGGGC TCTATGGCTT
 1021 TCTTGGAAGG CGAAAGAATC TGCAGGCCCA GCTGGCCGTA TCATGTCATA GCTGATTCCT
1081 GGTTGAATGT TATCCGCTTC ACAAATTTCC CAACGAACAT AG
//
Primer: BGHpA_F
Sequence file: RDB14100_A6Dcd.seq check
>D03255J1_A6Dc_BGHpA_F_E09_05_ABI08.ab1
    1 CGGGAGGGTT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
   61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
  121 AACCTGCAGC CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
  181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
  241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
  301 AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
  421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
  481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTAACTTG TTTATTGCAG
  541 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  601 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCC
  661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
  721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
  781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG ATAACGCACG AAAGAACATG
  841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
  901 CCATAGGCTC CGCCCCCCCT GACGAGCATC ACAAAAAATC GACGCTCAAG TCAGAGTGGC
  961 GAAACCCGAC AGACTATAAA GATACCAGGC GTTTCCCCCC TGGAAGCTCC CCTCGTGCGC
 1021 CTCCTCCTGG TTCCGACCCT GGCGCTACCG AATACCTGGT CGCTTTCTCC TCCGAAGCGT
1081 GGCGCCTTTC CCTCCATAAG C
//
Primer: Amp_R
Sequence file: RDB14100_A6Dce.seq check
>D03255J1_A6Dc_Amp_R_A07_01_ABI24.ab1
    1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
   61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
  121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
  181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
  241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
  301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
  361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
  421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
  481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
  541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
  601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
  661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
  721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
  781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
  841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
  901 CGCCAATAGG ACTTTCCATT TGACGTCATG GGTGGAGTAT TTACGGTAAC TGCCCACTTG
  961 CAGTACATCC AGTGTATCAT ATGGCAGGTA CGCCCCCTAT TGACGTCAAT GAACGTTAAT
 1021 TGACCGGCTG GACATATGGC AAGTTACATT GACTATACAC TACTACTTGC AGTACATCTA
1081 CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]
user_report Matsumoto, T., Androgen promotes squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway. Cancer Med. 12 (9): 10816-10828 (2023). PMID 36951594. [link to RRC of NBRP]
user_report Mise, Y., Immunosuppressive tumor microenvironment in Uterine Serous Carcinoma via CCL7 signal with myeloid-derived suppressor cells. Carcinogenesis bgac032 (2022). PMID 35353883. [link to RRC of NBRP]