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pCAGIPuro-FlagmcMycWT

Expression vector of FLAG tagged mouse cMyc, wild type.

Catalog number RDB14100
Resource name pCAGIPuro-FlagmcMycWT
Clone info. Expression vector of FLAG tagged mouse cMyc, wild type.
Comment Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone pCAG-IP (Plasmid)
Size of vector backbone 7.2 kb
Selectable markers Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name mouse Myc cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
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External Database
mouse Myc

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14100 pCAGIPuro-FlagmcMycWT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14100_A6Dcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14100_A6Dca.seq check
          >D03255J1_A6Dc_pAxCA_F1_E06_15_ABI24.ab1
              1 GTAACGGTCT GCTACATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
             61 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTCCT CGAGCCACCA TGGACTACAA
            121 GGACGACGAT GACAAGGGAG GAGGCATGCC CCTCAACGTG AACTTCACCA ACAGGAACTA
            181 TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC GACGAGGAAG AGAATTTCTA
            241 TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC AGTGAGGATA TCTGGAAGAA
            301 ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC CGCTCCGGGC TCTGCTCTCC
            361 ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA GACGATGACG GCGGCGGTGG
            421 CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG TTACTTGGAG GAGACATGGT
            481 GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC ATCAAGAACA TCATCATCCA
            541 GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG GTCTCGGAGA AGCTGGCCTC
            601 CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG
            661 CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC
            721 CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC
            781 CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG
            841 GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC
            901 TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC
            961 CCCTGCCAAG AGGTCGAGTC GGGCTCATCT CCATCCCGAG GCCACAGCAA AACCTCCGCC
           1021 ACAGCCCACT GGTCCCTCAG AGTGCAACGT CTCACTCACA GCACACTACG CGCACCCCTC
          1081 CACAGGAATG ACTATCAGCT GGCAGAGGTA AGTGACAAGT TGGCCAGGAA TCCTGA
          //
          Primer: IRES-R
          Sequence file: RDB14100_A6Dcb.seq check
          >D03255J1_A6Dc_IRES-R_F06_18_ABI24.ab1
              1 GCTAGGCGGC TTCGGCCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGCGGAAT
             61 TCGATATCAA GCTTATCGAG CGGCCGCTCG ATACCGTCGA TCTCAGTGGT ATTTGTGAGC
            121 CAGTTGGCCA CACCAGCCAC CACCTTCTGA TAGGCAGCCT GCACCTGAGG AGTGAATTCC
            181 TCGAGTTATG CACCAGAGTT TCGAAGCTGT TCGAGTTTGT GTTTCAATTG TTCTCGTCGT
            241 TTCCTCAATA AGTCCTTTTC AGAGGTGAGC TTGTGCTCGT CTGCTTGAAT GGACAGGATG
            301 TAGGCGGTGG CTTTTTTGAG GATCACTACC TTGGGGGCCT TTTCGTTGTT TTCCAATTCA
            361 GGGATCTGGT CACGCAGGGC AAAAAAGCTG CGCTTCAGCT CGTTCCTCCT CTGACGTTCC
            421 AAGACGTTGT GTGTCCGCCT CTTGTCGTTT TCCTCCGTGT CTGAGGACCT GGGGCTGGAG
            481 CACTTGCGGT TGTTGCTGAT CTGCTTCAGG ACCCTGCCAC TGTCCAACTT GGCCCTCTTG
            541 GCAGCTGGAT AGTCCTTCCT TGTGGAGGGG GGTGCGGCGT AGTTGTGCTG GTGAGTGGAG
            601 ACGTGGCACC TCTTGAGGAC CAGTGGGCTG TGCGGAGGTT TGCTGTGGCC TCGGGATGGA
            661 GATGAGCCCG ACTCCGACCT CTTGGCAGGG GTTTGCCTCT TCTCCACAGA CACCACATCA
            721 ATTTCTTCCT CATCTTCTTG CTCTTCTTCA GAGTCGCTGC TGGTGGTGGG CGGTGTCTCC
            781 TCATGCAGCA CTAGGGGCTC AGGCTGGCCC GTGGGGAGGA CTCGGAGGAC AGCAGCGAGT
            841 CCGAGGAAGG AGAGAAGGCC GTGGATCGGA CGAGTACAGG ATTTGGGCGA GCTGCTGTCG
            901 TGAGCGGTAG GGAAAGACCA CTGAGGGGTC ATGCACTCGA CGCGCGCGTG AGTCTGCAGT
            961 ACAGGCTGAG TGAGCGACGC TGTGCCGCGG CCGGGCTCAG CTGTGCTGCT TGCCGCAGCC
           1021 TGGTAGAAGC AGCTCCGGAC CAGCTTGCAC GGCTGAACGT CCATACAGTC TGGATGATGA
          1081 GTTTCCTTGT GA
          //
          Primer: IRES-F
          Sequence file: RDB14100_A6Dcc.seq check
          >D03255J1_A6Dc_IRES-F_G06_21_ABI24.ab1
              1 GCAGTCGTTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
             61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC
            121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
            181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
            241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
            301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
            361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
            421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
            481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
            541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
            601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
            661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC
            721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT
            781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
            841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
            901 CGCATTGTCT GAGTAGTGTC ATTCTATTCT GGGGGGGTGG GGGTGGGGCA GACAGCAAAG
            961 GGGAGGATTG GGAAGAACAA TTAGCAGGCA TGCTGGGGAT GCCGGTGGGC TCTATGGCTT
           1021 TCTTGGAAGG CGAAAGAATC TGCAGGCCCA GCTGGCCGTA TCATGTCATA GCTGATTCCT
          1081 GGTTGAATGT TATCCGCTTC ACAAATTTCC CAACGAACAT AG
          //
          Primer: BGHpA_F
          Sequence file: RDB14100_A6Dcd.seq check
          >D03255J1_A6Dc_BGHpA_F_E09_05_ABI08.ab1
              1 CGGGAGGGTT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG
             61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG
            121 AACCTGCAGC CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
            181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC
            241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG
            301 AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
            361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
            421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
            481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTAACTTG TTTATTGCAG
            541 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
            601 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCC
            661 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC
            721 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
            781 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG ATAACGCACG AAAGAACATG
            841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
            901 CCATAGGCTC CGCCCCCCCT GACGAGCATC ACAAAAAATC GACGCTCAAG TCAGAGTGGC
            961 GAAACCCGAC AGACTATAAA GATACCAGGC GTTTCCCCCC TGGAAGCTCC CCTCGTGCGC
           1021 CTCCTCCTGG TTCCGACCCT GGCGCTACCG AATACCTGGT CGCTTTCTCC TCCGAAGCGT
          1081 GGCGCCTTTC CCTCCATAAG C
          //
          Primer: Amp_R
          Sequence file: RDB14100_A6Dce.seq check
          >D03255J1_A6Dc_Amp_R_A07_01_ABI24.ab1
              1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC
             61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG
            121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC
            181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC
            241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG
            301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG
            361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT
            421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT
            481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA
            541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG
            601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
            661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT
            721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
            781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG
            841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA
            901 CGCCAATAGG ACTTTCCATT TGACGTCATG GGTGGAGTAT TTACGGTAAC TGCCCACTTG
            961 CAGTACATCC AGTGTATCAT ATGGCAGGTA CGCCCCCTAT TGACGTCAAT GAACGTTAAT
           1021 TGACCGGCTG GACATATGGC AAGTTACATT GACTATACAC TACTACTTGC AGTACATCTA
          1081 CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl