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pMXs-mJarid2DC-P2A-mcMycWT

Retroviral expression vector of F2A peptide sequence-linked cMyc and Jarid2 coexpression system.

Catalog number RDB14099
Resource name pMXs-mJarid2DC-P2A-mcMycWT
Alternative name clone 42
Clone info. Retroviral expression vector of P2A peptide sequence-linked Jarid2 and cMyc coexpression system, deletion of C-terminal of Jarid2 in Fig.1I and J (Iseki, H., Stem Cells 34, 322-333, 2016).
Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Myc cDNA mouse Jarid2 cDNA
Depositor Okazaki, Yasushi | Iseki, Hiroyoshi |
 
Other clones in our bank mouse Myc (NCBI Gene 17869) | mouse Jarid2 (NCBI Gene 16468) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Remarks Remember that you will be working with samples containing infectious virus.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14099 pMXs-mJarid2DC-P2A-mcMycWT Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

2019.07.19

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