RDB14058 - pMXs-mEzh2

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pMXs-mEzh2 (#RDB14058)

Retroviral expression vector of mouse Ezh2, variant 1.

Clone info.	Retroviral expression vector of mouse Ezh2, variant 1.
Comment	Expression was confirmed by the depositor.
Vector backbone	pMXs Retroviral Vector (Plasmid)
Size of vector backbone	5.9 kb
Selectable markers	Amp^r
Gene/insert name	mouse Ezh2 cDNA
Depositor\|Developer	Okazaki, Yasuji \| Iseki, Hiroyoshi \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015) Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。 MTAに書く付加的使用条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献（Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14058	pMXs-mEzh2	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-mEzh2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14058).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14058_A6Dcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 Sequence file: RDB14058_A6Dca.seq
>D03255E4_A6Dc_2_MMLV-pol_F2_F07_16.ab1 1 TAGGGGGGGT GGAAATCCTC TAGACTGCCG GATCTAGCTA GTTAATTAAG GATCCACCAT 61 GGGCCAGACT GGGAAGAAAT CTGAGAAGGG ACCGGTTTGT TGGCGGAAGC GTGTAAAATC 121 AGAGTACATG AGACTGAGAC AGCTCAAGAG GTTCAGAAGA GCTGATGAAG TAAAGACTAT 181 GTTTAGTTCC AATCGTCAGA AAATTTTGGA AAGAACTGAA ACCTTAAACC AAGAGTGGAA 241 GCAGCGGAGG ATACAGCCTG TGCACATCAT GACTTCTGTG AGCTCATTGC GCGGGACTAG 301 GGAGTGTTCA GTCACCAGTG ACTTGGATTT TCCAGCACAA GTCATCCCGT TAAAGACCCT 361 GAATGCAGTC GCCTCGGTGC CTATAATGTA CTCTTGGTCG CCCTTACAAC AGAATTTTAT 421 GGTGGAAGAC GAAACTGTTT TACATAACAT TCCTTATATG GGGGATGAAG TTCTGGATCA 481 GGATGGCACT TTCATTGAAG AACTAATAAA AAATTATGAT GGAAAAGTGC ATGGTGACAG 541 AGAATGTGGA TTTATAAATG ATGAAATTTT TGTGGAGTTG GTAAATGCTC TTGGTCAATA 601 TAATGATGAT GATGATGACG ATGATGGAGA TGATCCAGAT GAAAGAGAAG AAAAACAGAA 661 AGATCTAGAG GATAATCGAG ATGATAAAGA AACTTGCCCA CCTCGGAAAT TTCCTGCTGA 721 TAAAATATTT GAAGCCATTT CCTCAATGTT TCCAGATAAG GGCACCGCAG AAGAACTGAA 781 AGAAAAATAT AAAGAACTCA CGGAGCAGCA GCTCCCAGGT GCTCTGCCTC CTGAATGTAC 841 TCCAAACATC GATGGACCAA ATGCCAAATC TGTTCAGAGG GAGCAAAGCT TGCATTCATT 901 TCATACGCTC TTCTGTCGAC GATGTTTTAA GTATGACTGC TTCCTACATC CCTTCCATGC 961 AACACCCAAC ACATATAAGA GGAAGAACAC AGAAACAGCT TTGGACAACA AAGCCCTTTG 1021 TGGACCACAG TGTTTACCAG CATCTGGGAG GGAGCTAAAG AGTTTGCTGC TGCTCTTACT 1081 GCTGAGCGTA ATAAAAGAAC ACAACTTAAC GGCCAGTGCG CAGAGGAGGA AAGGACTTAC 1141 GAATTACG //
Primer: pMXs-3LTRup_R2 Sequence file: RDB14058_A6Dcb.seq
>D03255E4_A6Dc_2_pMXs-3LTRup_R2_G08_20.ab1 1 CTAATAAATC TTTTATTTTA TCGCGAGTCA AGGGATTTCC ATTTCTCGTT CGATGCCCAC 61 ATACTTCAGG GCATCAGCCT GGCTGTATCT GTAATCAAAA AACAACTCTT CACCAGTCTG 121 GATAGCCCTC TTAGCAAAGA TGCCTATCCT GTGGTCACCA TTAACCATCA TAACTTTTGC 181 ATAGCAGTTT GGATTTACTG AATGATTAGC AAAACGAATT TTGTTGCCCT TTCGGGTTGC 241 ATCCACCACA AAATCATTGT TCAAGTTGAA CAGAAAGCTG CACATGTATT TGTCATACAC 301 TTTTCCTCTT CTGTCTGCTT CATCCTGAGA AATAATCTCC CCACAGTATT CTGAGATGAA 361 TTCATTTTTC TGTACAGGAT CTTTGATAAA GATGCCCCAG CCTGCCACAT CAGACGGTGC 421 CAGCAGTAAG TGCTTTTTAG AGCCCCGCTG AATGCTACAG TTCTTACAGG ATACATTTTT 481 ACTGTCCCAA TGGTCAGCAG CTCCACACGT GAGACAGAGG TCAGGGTCAC ACTCTCGGAC 541 AGCCAGGTAG CATGGACACT GTTTGGTGTT GCATTGTGCT TTGCACCGAC ATCCAGGAAA 601 GCGGTTTTGA CCCTTTTTCA GTTGTATCTT TCTGCAGTGT GCAGCCCACA ACCGATGTTT 661 CCTTTTCTTC TTTCTTGGAG GAGTGTCTAC ATCCTCAGTG GGAACAGGTG CTATGATACT 721 GGACTCCTTG ACTCTAAACT CATACACCTG TCTACATGTT TTGGTCCCAA TTAGCCTAGC 781 AATGGCACAA AAGTTATCGT AGTAAGTACC AATGAGGACT CTAAACATGG AGGCTTCAGC 841 ACCACTCCAC TCCACATTCT CAGGAGGTTC AATATTTGGC TTCATCTTTA TTGGTGTTTG 901 ACACCGAGAA TTTGCTTCAG AGGAGCTGGA CGTCTCATCT TTTTTTCTCT TCTTCTTCTT 961 TATCATTGTT CTCTCCCCCC AGTTTCAGTC CCTGCTTCTC TGTCACTGTC TGTATCCCTT 1021 TGACTCCAGC ACACTGATGG TTGGGGTGCT GGGGTCTGCT ACTGGTTATT CGAAAGTCTT 1081 CCTCATCCTG CCGACCCCCT TGCGCCGATT TAAGGTGGTT GATCCTTTTA AATAAAGCG //
Primer: RDB14058#1 Sequence file: RDB14058_A6Dcc.seq
>D03255E4-2_A6Dc_RDB14058#1_D01_04_ABI08.ab1 1 GGGGCAGAGT TTTAGTATGA CTGCTTCCTA CATCCCTTCC ATGCAACACC CAACACATAT 61 AAGAGGAAGA ACACAGAAAC AGCTTTGGAC AACAAGCCTT GTGGACCACA GTGTTACCAG 121 CATCTGGAGG GAGCTAAGGA GTTTGCTGCT GCTCTTACTG CTGAGCGTAT AAAGACACCA 181 CCTAAACGCC CAGGGGGCCG CAGAAGAGGA AGACTTCCGA ATAACAGTAG CAGACCCAGC 241 ACCCCCACCA TCAGTGTGCT GGAGTCAAAG GATACAGACA GTGACAGAGA AGCAGGGACT 301 GAAACTGGGG GAGAGAACAA TGATAAAGAA GAAGAAGAGA AAAAAGATGA GACGTCCAGC 361 TCCTCTGAAG CAAATTCTCG GTGTCAAACA CCAATAAAGA TGAAGCCAAA TATTGAACCT 421 CCTGAGAATG TGGAGTGGAG TGGTGCTGAA GCCTCCATGT TTAGAGTCCT CATTGGTACT 481 TACTACGATA ACTTTTGTGC CATTGCTAGG CTAATTGGGA CCAAAACATG TAGACAGGTG 541 TATGAGTTTA GAGTCAAGGA GTCCAGTATC ATAGCACCTG TTCCCACTGA GGATGTAGAC 601 ACTCCTCCAA GAAAGAAGAA AAGGAAACAT CGGTTGTGGG CTGCACACTG CAGAAAGATA 661 CAACTGAAAA AGGGTCAAAA CCGCTTTCCT GGATGTCGGT GCAAAGCACA ATGCAACACC 721 AAACAGTGTC CATGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTCACGTGT 781 GGAGCTGCTG ACCATTGGGA CAGTAAAAAT GTATCCTGTA AGAACTGTAG CATTCAGCGG 841 GGCTCTAAAA AGCACTTACT GCTGGCACCG TCTGATGTGG CAGGCTGGGG CATCTTTATC 901 AAAGATCCTG TACAGAAAAA TGAATTCATC TCAGAATACT GTGGGGAGAT TATTTCTCAG 961 GATGAAGCAG ACAGAAGAGG AAAAGTGTAT GACAAATACA TGTGCAGCTT TCTGTTCAAC 1021 TTGAACAATG ATTTTGGTGG TGGATGCAAC CCGAAGGCAC AAATTCGGTT TTGCTTAATC 1081 ATTCAGTTAA ATCCAAACTG CTATGGCAAA GGTAATGGAA TGGATAAATG GGTTGACCCA 1141 CAGGAATAGG GCAATCTCTT TTGTTC //
Primer: Amp_R Sequence file: RDB14058_A6Dcd.seq
>D03255E4-2_A6Dc_Amp_R_A01_01_ABI08.ab1 1 TTTATTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT 181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC 241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG 301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG 361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA 421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG 481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC 541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG 601 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC 661 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC 721 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG 781 GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT 841 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT 901 GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG 961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA AATCCCGATC GTTTTGGACT CTTTGGTGCA 1021 CCCCCCTAAT AGGAGGATAT GTGGGTTCTG GGTAGAGACG AGACCCTAAA CAGTTCCGCT 1081 CGTCTGATTT TTGCTTCGGC TTGGACGAGC CGCGACGCGC CGTCCTTGTC TTGCTGCCAA 1141 GG //
Primer: pMFG_1 Sequence file: RDB14058_A6Dce.seq
>D03255E4-2_A6Dc_pMFG_1_B01_02_ABI08.ab1 1 GGATGGGGAA GGAAGGGGGC TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC 61 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG 121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG 181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA 241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG 301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA 361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC 421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA 481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA 541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC 601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT 661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA 721 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC 781 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA 841 TACAGAGCTA GTTAGCTAAC TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA 901 GACAATCGGA CAGACACAGA TAAGTTGCTG GCCAGCTTAC CTCCCGGGTG GTGGGTCGGT 961 GGTCCCTGGG CAGGGGTCTC CCGATCCCGG GACGAGCCCC CAAAATGAAA GACCCCCGCT 1021 GACGGGTAGT CAAATCACTC AGAAGGGAGA CCCTCCCAAG GGAACAAGCG AGGACCAACA 1081 AGTTCGGGAT GGCAACTGCA AGAGGGTTAT GATTACACGG GTACCGGGCC GACTCAAGTC 1141 AAATCGGGAG AAGGAACCTT GTGGCTGC //
Primer: SV40pA3'Element Reverse Sequence file: RDB14058_A6Dcf.seq
>D03255E4-2_A6Dc_SV40pA3'ElementReverse_C01_03_ABI08.ab1 1 GCGGGCCCAG GGGGGGCCGG GGGCTATGGA AACGCCAGCA ACGCGGCCTT TTTACGGTTC 61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG 121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG 181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC 241 GCGCGTTGGC CGATTCATTA ATGCAACTAT GGCCATTTAA TGTAAATACT TAAGAAAAAA 301 AACCAAATTA ATTTTGATAC ATGCTGCATG TGAAAGACCC CCGCTGACGG GTAGTCAATC 361 ACTCAGAGGA GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG 421 GTTTATTGGA TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCCCCGAGT 481 GAGGGGTTGT GGGCTCTTTT ATTGAGCTCG GGGAGCAGAA GCGCGCGAAC AGAAGCGAGA 541 AGCGAACTGA TTGGTTAGTT CAAATAAGGC ACAGGGTCAT TTCAGGTCCT TGGGGCACCC 601 TGGAAACATC TGATGGTTCT CTAGAAACTG CTGAGGGCTG GACCGCATCT GGGGACCATC 661 TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT TTGGCCCATA 721 TTCAGCTGTT CCATCTGTTC TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT 781 CCTGTTTGGC CCATATTCAG CTGTTCCATC TGTTCCTGAC CTTGATCTGA ACTTCTCTAT 841 TCTCAGTTAT GTATTTTTCC ATGCCTTGCA AAATGGCGTT ACTTAAGCTA GCTTGCCAAA 901 CCTACAGGTG GGGTCTTTCA TTCCCCCCCT TTTTTCTGGA GACTAAATAA AATCTTTTAT 961 TTTATCGCGA GTCAAGGGGA ATTTCCATTT CTCGTTCGAT GCCCACATAC TTTCAAGGGC 1021 ATCAGCCCTG GCTGTATCTG TAATCAAAAA ACAACTCTTC CACCAGTCTG GGATAGTCCT 1081 TCTTTAGCAA AGATGTCTAA TCTTGTTGTT CACCATTAGC CCATCCATAA CCGTTTTGGC 1141 ATATAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946. [link to RRC of NBRP]

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mouse Ezh2

External Database
mouse Ezh2

Reference sequence