Resource data sheet
Prev.

pMXs-mEzh2

Retroviral expression vector of mouse Ezh2, variant 1.

Catalog number RDB14058
Resource name pMXs-mEzh2
Clone info. Retroviral expression vector of mouse Ezh2, variant 1.
Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Ezh2 cDNA
Depositor Okazaki, Yasushi | Iseki, Hiroyoshi |
 
Other clones in our bank mouse Ezh2 (NCBI Gene 14056) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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RDB14058 pMXs-mEzh2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14058_A6Dcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F2 RDB14058_A6Dca.seq
>D03255E4_A6Dc_2_MMLV-pol_F2_F07_16.ab1
    1 TAGGGGGGGT GGAAATCCTC TAGACTGCCG GATCTAGCTA GTTAATTAAG GATCCACCAT
   61 GGGCCAGACT GGGAAGAAAT CTGAGAAGGG ACCGGTTTGT TGGCGGAAGC GTGTAAAATC
  121 AGAGTACATG AGACTGAGAC AGCTCAAGAG GTTCAGAAGA GCTGATGAAG TAAAGACTAT
  181 GTTTAGTTCC AATCGTCAGA AAATTTTGGA AAGAACTGAA ACCTTAAACC AAGAGTGGAA
  241 GCAGCGGAGG ATACAGCCTG TGCACATCAT GACTTCTGTG AGCTCATTGC GCGGGACTAG
  301 GGAGTGTTCA GTCACCAGTG ACTTGGATTT TCCAGCACAA GTCATCCCGT TAAAGACCCT
  361 GAATGCAGTC GCCTCGGTGC CTATAATGTA CTCTTGGTCG CCCTTACAAC AGAATTTTAT
  421 GGTGGAAGAC GAAACTGTTT TACATAACAT TCCTTATATG GGGGATGAAG TTCTGGATCA
  481 GGATGGCACT TTCATTGAAG AACTAATAAA AAATTATGAT GGAAAAGTGC ATGGTGACAG
  541 AGAATGTGGA TTTATAAATG ATGAAATTTT TGTGGAGTTG GTAAATGCTC TTGGTCAATA
  601 TAATGATGAT GATGATGACG ATGATGGAGA TGATCCAGAT GAAAGAGAAG AAAAACAGAA
  661 AGATCTAGAG GATAATCGAG ATGATAAAGA AACTTGCCCA CCTCGGAAAT TTCCTGCTGA
  721 TAAAATATTT GAAGCCATTT CCTCAATGTT TCCAGATAAG GGCACCGCAG AAGAACTGAA
  781 AGAAAAATAT AAAGAACTCA CGGAGCAGCA GCTCCCAGGT GCTCTGCCTC CTGAATGTAC
  841 TCCAAACATC GATGGACCAA ATGCCAAATC TGTTCAGAGG GAGCAAAGCT TGCATTCATT
  901 TCATACGCTC TTCTGTCGAC GATGTTTTAA GTATGACTGC TTCCTACATC CCTTCCATGC
  961 AACACCCAAC ACATATAAGA GGAAGAACAC AGAAACAGCT TTGGACAACA AAGCCCTTTG
 1021 TGGACCACAG TGTTTACCAG CATCTGGGAG GGAGCTAAAG AGTTTGCTGC TGCTCTTACT
 1081 GCTGAGCGTA ATAAAAGAAC ACAACTTAAC GGCCAGTGCG CAGAGGAGGA AAGGACTTAC
1141 GAATTACG
//
Primer: pMXs-3LTRup_R2 RDB14058_A6Dcb.seq
>D03255E4_A6Dc_2_pMXs-3LTRup_R2_G08_20.ab1
    1 CTAATAAATC TTTTATTTTA TCGCGAGTCA AGGGATTTCC ATTTCTCGTT CGATGCCCAC
   61 ATACTTCAGG GCATCAGCCT GGCTGTATCT GTAATCAAAA AACAACTCTT CACCAGTCTG
  121 GATAGCCCTC TTAGCAAAGA TGCCTATCCT GTGGTCACCA TTAACCATCA TAACTTTTGC
  181 ATAGCAGTTT GGATTTACTG AATGATTAGC AAAACGAATT TTGTTGCCCT TTCGGGTTGC
  241 ATCCACCACA AAATCATTGT TCAAGTTGAA CAGAAAGCTG CACATGTATT TGTCATACAC
  301 TTTTCCTCTT CTGTCTGCTT CATCCTGAGA AATAATCTCC CCACAGTATT CTGAGATGAA
  361 TTCATTTTTC TGTACAGGAT CTTTGATAAA GATGCCCCAG CCTGCCACAT CAGACGGTGC
  421 CAGCAGTAAG TGCTTTTTAG AGCCCCGCTG AATGCTACAG TTCTTACAGG ATACATTTTT
  481 ACTGTCCCAA TGGTCAGCAG CTCCACACGT GAGACAGAGG TCAGGGTCAC ACTCTCGGAC
  541 AGCCAGGTAG CATGGACACT GTTTGGTGTT GCATTGTGCT TTGCACCGAC ATCCAGGAAA
  601 GCGGTTTTGA CCCTTTTTCA GTTGTATCTT TCTGCAGTGT GCAGCCCACA ACCGATGTTT
  661 CCTTTTCTTC TTTCTTGGAG GAGTGTCTAC ATCCTCAGTG GGAACAGGTG CTATGATACT
  721 GGACTCCTTG ACTCTAAACT CATACACCTG TCTACATGTT TTGGTCCCAA TTAGCCTAGC
  781 AATGGCACAA AAGTTATCGT AGTAAGTACC AATGAGGACT CTAAACATGG AGGCTTCAGC
  841 ACCACTCCAC TCCACATTCT CAGGAGGTTC AATATTTGGC TTCATCTTTA TTGGTGTTTG
  901 ACACCGAGAA TTTGCTTCAG AGGAGCTGGA CGTCTCATCT TTTTTTCTCT TCTTCTTCTT
  961 TATCATTGTT CTCTCCCCCC AGTTTCAGTC CCTGCTTCTC TGTCACTGTC TGTATCCCTT
 1021 TGACTCCAGC ACACTGATGG TTGGGGTGCT GGGGTCTGCT ACTGGTTATT CGAAAGTCTT
1081 CCTCATCCTG CCGACCCCCT TGCGCCGATT TAAGGTGGTT GATCCTTTTA AATAAAGCG
//
Primer: RDB14058#1 RDB14058_A6Dcc.seq
>D03255E4-2_A6Dc_RDB14058#1_D01_04_ABI08.ab1
    1 GGGGCAGAGT TTTAGTATGA CTGCTTCCTA CATCCCTTCC ATGCAACACC CAACACATAT
   61 AAGAGGAAGA ACACAGAAAC AGCTTTGGAC AACAAGCCTT GTGGACCACA GTGTTACCAG
  121 CATCTGGAGG GAGCTAAGGA GTTTGCTGCT GCTCTTACTG CTGAGCGTAT AAAGACACCA
  181 CCTAAACGCC CAGGGGGCCG CAGAAGAGGA AGACTTCCGA ATAACAGTAG CAGACCCAGC
  241 ACCCCCACCA TCAGTGTGCT GGAGTCAAAG GATACAGACA GTGACAGAGA AGCAGGGACT
  301 GAAACTGGGG GAGAGAACAA TGATAAAGAA GAAGAAGAGA AAAAAGATGA GACGTCCAGC
  361 TCCTCTGAAG CAAATTCTCG GTGTCAAACA CCAATAAAGA TGAAGCCAAA TATTGAACCT
  421 CCTGAGAATG TGGAGTGGAG TGGTGCTGAA GCCTCCATGT TTAGAGTCCT CATTGGTACT
  481 TACTACGATA ACTTTTGTGC CATTGCTAGG CTAATTGGGA CCAAAACATG TAGACAGGTG
  541 TATGAGTTTA GAGTCAAGGA GTCCAGTATC ATAGCACCTG TTCCCACTGA GGATGTAGAC
  601 ACTCCTCCAA GAAAGAAGAA AAGGAAACAT CGGTTGTGGG CTGCACACTG CAGAAAGATA
  661 CAACTGAAAA AGGGTCAAAA CCGCTTTCCT GGATGTCGGT GCAAAGCACA ATGCAACACC
  721 AAACAGTGTC CATGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTCACGTGT
  781 GGAGCTGCTG ACCATTGGGA CAGTAAAAAT GTATCCTGTA AGAACTGTAG CATTCAGCGG
  841 GGCTCTAAAA AGCACTTACT GCTGGCACCG TCTGATGTGG CAGGCTGGGG CATCTTTATC
  901 AAAGATCCTG TACAGAAAAA TGAATTCATC TCAGAATACT GTGGGGAGAT TATTTCTCAG
  961 GATGAAGCAG ACAGAAGAGG AAAAGTGTAT GACAAATACA TGTGCAGCTT TCTGTTCAAC
 1021 TTGAACAATG ATTTTGGTGG TGGATGCAAC CCGAAGGCAC AAATTCGGTT TTGCTTAATC
 1081 ATTCAGTTAA ATCCAAACTG CTATGGCAAA GGTAATGGAA TGGATAAATG GGTTGACCCA
1141 CAGGAATAGG GCAATCTCTT TTGTTC
//
Primer: Amp_R RDB14058_A6Dcd.seq
>D03255E4-2_A6Dc_Amp_R_A01_01_ABI08.ab1
    1 TTTATTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT
  181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC
  241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
  301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
  361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
  421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
  481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
  541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG
  601 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
  661 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
  721 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
  781 GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
  841 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
  901 GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
  961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA AATCCCGATC GTTTTGGACT CTTTGGTGCA
 1021 CCCCCCTAAT AGGAGGATAT GTGGGTTCTG GGTAGAGACG AGACCCTAAA CAGTTCCGCT
 1081 CGTCTGATTT TTGCTTCGGC TTGGACGAGC CGCGACGCGC CGTCCTTGTC TTGCTGCCAA
1141 GG
//
Primer: pMFG_1 RDB14058_A6Dce.seq
>D03255E4-2_A6Dc_pMFG_1_B01_02_ABI08.ab1
    1 GGATGGGGAA GGAAGGGGGC TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC
   61 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
  121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
  181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
  241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
  301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
  361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
  421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
  481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
  541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
  601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT
  661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
  721 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC
  781 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA
  841 TACAGAGCTA GTTAGCTAAC TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA
  901 GACAATCGGA CAGACACAGA TAAGTTGCTG GCCAGCTTAC CTCCCGGGTG GTGGGTCGGT
  961 GGTCCCTGGG CAGGGGTCTC CCGATCCCGG GACGAGCCCC CAAAATGAAA GACCCCCGCT
 1021 GACGGGTAGT CAAATCACTC AGAAGGGAGA CCCTCCCAAG GGAACAAGCG AGGACCAACA
 1081 AGTTCGGGAT GGCAACTGCA AGAGGGTTAT GATTACACGG GTACCGGGCC GACTCAAGTC
1141 AAATCGGGAG AAGGAACCTT GTGGCTGC
//
Primer: SV40pA3'Element Reverse RDB14058_A6Dcf.seq
>D03255E4-2_A6Dc_SV40pA3'ElementReverse_C01_03_ABI08.ab1
    1 GCGGGCCCAG GGGGGGCCGG GGGCTATGGA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
   61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
  121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
  181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
  241 GCGCGTTGGC CGATTCATTA ATGCAACTAT GGCCATTTAA TGTAAATACT TAAGAAAAAA
  301 AACCAAATTA ATTTTGATAC ATGCTGCATG TGAAAGACCC CCGCTGACGG GTAGTCAATC
  361 ACTCAGAGGA GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG
  421 GTTTATTGGA TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCCCCGAGT
  481 GAGGGGTTGT GGGCTCTTTT ATTGAGCTCG GGGAGCAGAA GCGCGCGAAC AGAAGCGAGA
  541 AGCGAACTGA TTGGTTAGTT CAAATAAGGC ACAGGGTCAT TTCAGGTCCT TGGGGCACCC
  601 TGGAAACATC TGATGGTTCT CTAGAAACTG CTGAGGGCTG GACCGCATCT GGGGACCATC
  661 TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT TTGGCCCATA
  721 TTCAGCTGTT CCATCTGTTC TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT
  781 CCTGTTTGGC CCATATTCAG CTGTTCCATC TGTTCCTGAC CTTGATCTGA ACTTCTCTAT
  841 TCTCAGTTAT GTATTTTTCC ATGCCTTGCA AAATGGCGTT ACTTAAGCTA GCTTGCCAAA
  901 CCTACAGGTG GGGTCTTTCA TTCCCCCCCT TTTTTCTGGA GACTAAATAA AATCTTTTAT
  961 TTTATCGCGA GTCAAGGGGA ATTTCCATTT CTCGTTCGAT GCCCACATAC TTTCAAGGGC
 1021 ATCAGCCCTG GCTGTATCTG TAATCAAAAA ACAACTCTTC CACCAGTCTG GGATAGTCCT
 1081 TCTTTAGCAA AGATGTCTAA TCTTGTTGTT CACCATTAGC CCATCCATAA CCGTTTTGGC
1141 ATATAGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl