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pgRNA_mMecp2_T2 (#RDB14015)

Expression vector of sgRNA of mouse Mecp2.

Clone info. Expression vector of sgRNA for CRISPR-CAS9, targeting intron adjacent to 5'exon2 of mouse Mecp2.
Comment Expression was confirmed by the depositor.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mecp2 genomic DNA human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する ((Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14015 pgRNA_mMecp2_T2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pgRNA_mMecp2_T2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14015).

Reference section:

Tsuchiya, Y., Minami, Y., Umemura, Y., Watanabe, H., Ono, D., Nakamura, W., Takahashi, T., Honma, S., Kondoh, G., Matsuishi, T., Yagita, K., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14015_A6D7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB14015_A6D7a.seq check
>D03336A3_A6D7_1_T7_20bp_C11_08.ab1
    1 TCTACAGCAT GCTCGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC GCCCTTTGTA
   61 CAAAAAAGCA GGCTTTAAAG GAACCAATTC AGTCGACTGG ATCCGGTACC AAGGTCGGGC
  121 AGGAAGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC AAGGCTGTTA
  181 GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA AATACGTGAC
  241 GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT AAAATGGACT
  301 ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA
  361 AAGGACGAAA CACCGTGGGC CCCAGCTTGA CCCAGTTTTA GAGCTAGAAA TAGCAAGTTA
  421 AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTCTA
  481 GACCCAGCTT TCTTGTACAA AGTTGGCATT AAAGGGCGAA TTCCAGCACA CTGGCGGCCG
  541 TTACTAGTGG ATCCGAGCTC GGTACCAAGC TTGATGCATA GCTTGAGTAT TCTATAGTGT
  601 CACCTAAATA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
  661 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
  721 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
  781 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGGAGAGGCG GTTTGCGTAT
  841 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
  901 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAC GGGATAACGC
  961 AGGAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGG ACCGTAAAAA GGCCGCGTTG
 1021 CTGGCGTTTT TCATAGGCTC GCCCCCCTGA CGAGCCATCA CAAAATCGAC GCTCAGTCAG
1081 AGGTTGGCCG AACCTCGACA GGCACTATAA AGGAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]