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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pgRNA_mMecp2_T2

Expression vector of sgRNA of mouse Mecp2.

Catalog number RDB14015
Resource name pgRNA_mMecp2_T2
Clone info. Expression vector of sgRNA for CRISPR-CAS9, targeting intron adjacent to 5'exon2 of mouse Mecp2.
Comment Expression was confirmed by the depositor.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Mecp2 genomic DNA human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
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External Database
mouse Mecp2 human U6
        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する ((Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。
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        RDB14015 pgRNA_mMecp2_T2 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390.

        Further references such as user reports and related articles (go to bottom)

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        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14015_A6D7p1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: T7_20bp
        Sequence file: RDB14015_A6D7a.seq check
        >D03336A3_A6D7_1_T7_20bp_C11_08.ab1
            1 TCTACAGCAT GCTCGAGCGG CCGCCAGTGT GATGGATATC TGCAGAATTC GCCCTTTGTA
           61 CAAAAAAGCA GGCTTTAAAG GAACCAATTC AGTCGACTGG ATCCGGTACC AAGGTCGGGC
          121 AGGAAGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC AAGGCTGTTA
          181 GAGAGATAAT TAGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA AATACGTGAC
          241 GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT AAAATGGACT
          301 ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA
          361 AAGGACGAAA CACCGTGGGC CCCAGCTTGA CCCAGTTTTA GAGCTAGAAA TAGCAAGTTA
          421 AAATAAGGCT AGTCCGTTAT CAACTTGAAA AAGTGGCACC GAGTCGGTGC TTTTTTTCTA
          481 GACCCAGCTT TCTTGTACAA AGTTGGCATT AAAGGGCGAA TTCCAGCACA CTGGCGGCCG
          541 TTACTAGTGG ATCCGAGCTC GGTACCAAGC TTGATGCATA GCTTGAGTAT TCTATAGTGT
          601 CACCTAAATA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
          661 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
          721 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
          781 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGGAGAGGCG GTTTGCGTAT
          841 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
          901 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAC GGGATAACGC
          961 AGGAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGG ACCGTAAAAA GGCCGCGTTG
         1021 CTGGCGTTTT TCATAGGCTC GCCCCCCTGA CGAGCCATCA CAAAATCGAC GCTCAGTCAG
        1081 AGGTTGGCCG AACCTCGACA GGCACTATAA AGGAT
        //

        Please visit Sequencing and PCR primers for primer information.

        References

        Original, user report and related articles

        original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390.

        2022.05.17

        GNP_filter3_RDBDEP_html_220516.pl