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pgRNA_cloning_BbsI

Expression vector backbone of sgRNA with U6 promoter.

Catalog number RDB14013
Resource name pgRNA_cloning_BbsI
Clone info. Cloning vector of sgRNA for CRISPR-CAS9, containg U6 promoter and Bbs I sites for cloning of annealed oligo DNA for the sgRNA-targeting site.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
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External Database
human U6
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。
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          RDB14013 pgRNA_cloning_BbsI DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pgRNA_cloning_BbsI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14013).

          Reference section:

          Tsuchiya, Y., Minami, Y., Umemura, Y., Watanabe, H., Ono, D., Nakamura, W., Takahashi, T., Honma, S., Kondoh, G., Matsuishi, T., Yagita, K., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14013_A6D7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB14013_A6D7a.seq check
          >D03336A1_A6D7_1_T7_20bp_A11_02.ab1
              1 GGTTTTGAAT GCTTGCCTCG GAGCGGGCCG CCCAGTGTGA TGGATATCTG CAGAATTCGC
             61 CCTTTGTACA AAAAAGCAGG CTTTAAAGGA ACCAATTCAG TCGACTGGAT CCGGTACCAA
            121 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
            181 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
            241 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
            301 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
            361 CTTGTGGAAA GGACGAAACA CCGGGTCTTC GAGAAGACCT GTTTTAGAGC TAGAAATAGC
            421 AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT
            481 TTTCTAGACC CAGCTTTCTT GTACAAAGTT GGCATTAAAG GGCGAATTCC AGCACACTGG
            541 CGGCCGTTAC TAGTGGATCC GAGCTCGGTA CCAAGCTTGA TGCATAGCTT GAGTATTCTA
            601 TAGTGTCACC TAAATAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
            661 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
            721 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
            781 GGAAACCTGT CGTGCCAGCT GCATTAATGA AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
            841 GCGTATTGGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGGCTC GGTCGTTTCG
            901 GCTGCGGCGA GCGGTTATCA GCTCACTCAA GGGCGGTAAT ACCGGTTATC CACAGAATCA
            961 GGGAATAAAC GCAAGGAAAG AACATGTGAG CAAAAAGGCC AGCCAAAAGG CCAGGAACCC
           1021 GTAAAAAAGG GCCGGCGTTG CCTGGGCGTT TTTCCATAGG CTTCCGTCCC CCCTGAACGA
           1081 AGCATCCACA AAAATTCGAC GCTCAAGTTC GAGTGCGAAA CTCGACAGGA ACTTATAAAG
          1141 AGAAATACTC AGGCGCGGTT ATC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl