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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pgRNA_cloning_BbsI (#RDB14013)

Expression vector backbone of sgRNA with U6 promoter.

Clone info. Cloning vector of sgRNA for CRISPR-CAS9, containg U6 promoter and Bbs I sites for cloning of annealed oligo DNA for the sgRNA-targeting site.
Vector backbone gRNA_AAVS1-T1 (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human U6 genomic DNA
Depositor|Developer Yagita, Kazuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuchiya, Y. et al., Genes Cells 20, 992-1005, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14013 pgRNA_cloning_BbsI DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pgRNA_cloning_BbsI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14013).

Reference section:

Tsuchiya, Y., Minami, Y., Umemura, Y., Watanabe, H., Ono, D., Nakamura, W., Takahashi, T., Honma, S., Kondoh, G., Matsuishi, T., Yagita, K., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14013_A6D7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB14013_A6D7a.seq check
>D03336A1_A6D7_1_T7_20bp_A11_02.ab1
    1 GGTTTTGAAT GCTTGCCTCG GAGCGGGCCG CCCAGTGTGA TGGATATCTG CAGAATTCGC
   61 CCTTTGTACA AAAAAGCAGG CTTTAAAGGA ACCAATTCAG TCGACTGGAT CCGGTACCAA
  121 GGTCGGGCAG GAAGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA
  181 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA
  241 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA
  301 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
  361 CTTGTGGAAA GGACGAAACA CCGGGTCTTC GAGAAGACCT GTTTTAGAGC TAGAAATAGC
  421 AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT CGGTGCTTTT
  481 TTTCTAGACC CAGCTTTCTT GTACAAAGTT GGCATTAAAG GGCGAATTCC AGCACACTGG
  541 CGGCCGTTAC TAGTGGATCC GAGCTCGGTA CCAAGCTTGA TGCATAGCTT GAGTATTCTA
  601 TAGTGTCACC TAAATAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT
  661 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT
  721 GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG
  781 GGAAACCTGT CGTGCCAGCT GCATTAATGA AATCGGCCAA CGCGCGGGGA GAGGCGGTTT
  841 GCGTATTGGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGGCTC GGTCGTTTCG
  901 GCTGCGGCGA GCGGTTATCA GCTCACTCAA GGGCGGTAAT ACCGGTTATC CACAGAATCA
  961 GGGAATAAAC GCAAGGAAAG AACATGTGAG CAAAAAGGCC AGCCAAAAGG CCAGGAACCC
 1021 GTAAAAAAGG GCCGGCGTTG CCTGGGCGTT TTTCCATAGG CTTCCGTCCC CCCTGAACGA
 1081 AGCATCCACA AAAATTCGAC GCTCAAGTTC GAGTGCGAAA CTCGACAGGA ACTTATAAAG
1141 AGAAATACTC AGGCGCGGTT ATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuchiya, Y., Disruption of MeCP2 attenuates circadian rhythm in CRISPR/Cas9-based Rett syndrome model mouse. Genes Cells 20 (12): 992-1005 (2015). PMID 26456390. [link to RRC of NBRP]