Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-FLAG-HERC2 (#RDB13979)

Expression vector of FLAG-tagged human HERC2.

Clone info. Expression vector of FLAG-tagged human HERC2.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human HERC2 cDNA
Depositor|Developer Imai, Yuzuru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13979 pcDNA3-FLAG-HERC2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-FLAG-HERC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13979).

Reference section:

Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13979_A6C2p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,FLAG,insert 5' (hHERC2)
Sequence file: RDB13979_A6C2a.seq check
>D03172A7_A6C2_3_CMV_Forward_E02_14.ab1
    1 ATTATCCAAT TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTTG GTACCGAGCT CGGATCTCCG CCATGGACTA
  181 CAAGGACGAC GATGACAAGC CGGCCGCGGA TCTGGAATTC CTCGAGATGC CCTCTGAATC
  241 TTTCTGTTTG GCTGCCCAGG CTCGCCTCGA CTCCAAATGG TTGAAAACAG ATATACAGCT
  301 TGCATTCACA AGAGATGGGC TCTGTGGTCT GTGGAATGAA ATGGTTAAAG ATGGAGAAAT
  361 TGTATACACT GGAACAGAAT CAACCCAGAA CGGAGAGCTC CCTCCTAGAA AAGATGATAG
  421 TGTCGAACCA AGTGGAACAA AGAAAGAAGA TCTGAATGAC AAAGAGAAAA AAGATGAAGA
  481 AGAAACTCCT GCACCTATAT ATAGGGCCAA GTCAATTCTG GACAGCTGGG TATGGGGCAA
  541 GCAACCAGAT GTGAATGAAC TGAAGGAGTG TCTTTCTGTG CTGGTTAAAG AGCAGCAGGC
  601 CCTGGCCGTC CAGTCAGCCA CCACCACCCT CTCAGCCCTG CGACTCAAGC AGAGGCTGGT
  661 GATCTTGGAG CGCTATTTCA TTGCCTTGAA TAGAACCGTT TTTCAGGAGA ATGTCAAAGT
  721 TAAGTGGAAA AGCAGCGGTA TTTCTCTGCC TCCTGTGGAC AAAAAAAGTT CCCGGCCTGC
  781 GGGCAAAGGT GTGGAGGGGC TCGCCAGAGT GGGATCCCGA GCGGCGCTGT CTTTTGCCTT
  841 TGCCTTCCTG CGCAGGGCCT GGCGATCAGG CGAGGATGCG GACCTCTGCA GTGAGCTGTT
  901 GCACGAGTCC CTGGACGCCC TGCGAGCACT TCCCGAGGCC CTCGCTCTTT GACGAGAGCA
  961 CCGTGTCCTC TGTGTGGCTG GAGGTGGTGG AGAGAGCGAC CCAGGTTTCC TCAGTCCCGT
 1021 CGTGACGGGG ATGGTTCACG GAAACGCCAG CACTAAGGAT CAAGAAGCAT CCCCCTTGCA
1081 GGACAAGCAC TGGTCCTTGG CTATCTGCTG AACTGACTGG TGTGCCAG
//
Primer: BGH_rev2 (Pr0606)
Region: bGHpA,insert 3' (hHERC2)
Sequence file: RDB13979_A6C2b.seq check
>D03172A7_A6C2_3_BGH_rev2_F02_17.ab1
    1 CCAATGCCGG GCTTAAGGGA GAGCACGGGG GGAGGGGCAA ACAAACAGAT GGCTGGCAAA
   61 CTAGAAGGCA CAGTCGAGGC TGATCAGCGA GCTCTAGCAT TTAGGTGACT ATAGAATAGG
  121 GCCCTCTAGA TGCATGCTCG ACGCGGCCGC TTAGTGTCCT GTTAAATAAT CTTGTGTAGA
  181 GTCCGAAGCA AAGGAGTCGA CATCCTCGTT ATCTGAATCG TCGCTGCTGT CGTCGGCGGC
  241 TGGCTCTCCT GTAAGTGCGA TGCGAGCGTA GTCATCTGTG TCTATGGACT TGCAGAAGTG
  301 GATGGCGTAC TTGAGCTTCT CCTCCAGCAC CTGCTTGCAG GAATACCTGG GCAGCTTCAG
  361 CAAGAAGAAA CAGGTGTAGG ACTCAGGGAG GAAGTGGTCT GGAGGGTTGT ATTTATCCAA
  421 CACCTGGATG ACGAAGTCTC GGCCCCGGAA GTCGGCGATG GTCCTGGGCA GCCTCGTCCG
  481 GCCCCAGACG AAGCGAAGGA AAAGAGAGCG CTCTGTGTTG GAGAAGGACT CCATCACCTC
  541 CCAGAACCAC TGGATCAGCG ATGCGGAAGG CTCGATGCCT TTATAGGTGG CCACCGACTT
  601 GAGAAGGTGC AGCGGGATGT CAGGGCTGCC ACACACCATC GTCTCCAGTT CGTAGCCGGT
  661 GAACAGAGAG AGGGAGGGGA ACAGGCACAA CGCGGGCCAT TCCTTCCCGA ACAGCAGCCA
  721 CCTGCTCATC AAATTCATGG AGTCTATAGT TTATCGCCAG CCGCACGTAC TCCGCGCGGT
  781 TGTCCAGGGT GATGTGTGTG TGCTTGGAGC TCAACTGAAT GTCCTGGGCC ACTGGCACTT
  841 GGGCACTGTG AAAGGGCAGG CTCATGGCTT TCAAAACTCC TCTGAGGGTG GGCTTCATTG
  901 TCTCGGGATG TACATGAAGT CCAGGAAATA AAAATCCCTT ATCAACCCTC ACTGAAGGTC
  961 CGGCGATGGG TGAAGGCTCA TCCCAAGCCA GCCTGCTTTC AAGACAGGGC CTCGGGCAAG
 1021 GTTGGAGGCT CAAGGGGGAC TTCCCGGTTC GGAATGGCAA TGCCCAGCAA CCACACCCCA
 1081 GAAGCGGGAC AATGCTGGCT GGGTGCCACG GGTGGCTCTG GCCGGTCCGG GCCTTGAGAC
1141 CAGGGTAGAC CAAGTTCTTT CG
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB13979_A6C2c.seq check
>D03172A7_A6C2_3_SV40pro_F_G02_20.ab1
    1 GCCCGGTCCC TTGACTTTCC AGAATAGTGG AGGGAGGCTT TTTTGGAGGG CCTAAGGCTT
   61 TTGCAAAAAG CTCCCGGGAG GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT
  121 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG
  181 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG
  241 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG
  301 CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC
  361 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG
  421 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA
  481 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC
  541 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG
  601 ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG
  661 CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA
  721 TCATGGTGGA AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG
  781 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA
  841 TGGGGCTGAC CGCTTCCCTC GGTGCTTTAC GGGTATCGCC GCTCCCGGAT TCGCAGCGGC
  901 ATCGCCTTCT ATCGCCCTTC TTGACGAAGT TCTTTCTGAG CGGGGACTCC TGGGGGTTCG
  961 AAAATGACCG ACCCAAGCCG AACGCCCAAC CCTGCCCAAT CACGAGATTT CGGATTTCCA
 1021 ACCCGGCCCG GCCTTCTAAT GAAAGGGTTG GGGCCTTCCG GGAAATCGTT TTTTCCGGGG
 1081 GAAGCCGGCC TGGGATGGAA TCCCTCCCAG CGCGGGGGAA TCCTCAATGC TGGGGAGCTT
1141 ATTCGGCCTC ACACCCTCCA AACTTGTGTT CATAATGTGC GACAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]