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pcDNA3-NEURL4-FLAG-His (#RDB13978)

Expression vector of FLAG-His-tagged human NEURAL4.

Clone info. Expression vector of FLAG-His-tagged human NEURAL4.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human NEURL4 cDNA
Depositor|Developer Imai, Yuzuru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13978 pcDNA3-NEURL4-FLAG-His DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-NEURL4-FLAG-His was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13978).

Reference section:

Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13978_A6C2p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13978_A6C2a.seq check
>D03172A6_A6C2_2_CMV_Forward_E12_15.ab1
    1 AGTCACCTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTGGTACCG AGCTCGGATC CACTAGTAAC GGCCGCCAGT
  181 GTGCTGGAAT TCCTCGACAT GGCGGCAGGG TCGGGTGGGA GTGGGGGCTC TGGGGGAGGC
  241 CCTGGACCGG GGCCGGGCGG GGGTGGGGGC CCCAGCGGGA GCGGCTCAGG ACCGGGGTCC
  301 AACGGGGGTC TGGGCAGCGG CGGGGAACTG CACCCGCGCA CTGGGCGCTT GGTGAGCCTG
  361 TCGGCCTGTG GGCGTACGGC GCGGCGGCAG CAGCCGGGCC AGGAGTTTAA CCACGGGCTG
  421 GTGTTGAGCC GAGAACCCTT GCGCGATGGA CGCGTCTTCA CCGTCCGCAT CGACCGCAAG
  481 GTCAACTCCT GGAGCGGCTC CATTGAGATT GGGGTGACAG CGCTGGACCC CAGTGTGCTG
  541 GACTTTCCAA GCAGTGCCAC GGGCCTGAAA GGGGGCTCGT GGGTAGTGTC GGGCTGCTCT
  601 GTGCTGAGAG ATGGACGCTC TGTGTTGGAG GAGTATGGTC AGGACCTGGA CCAGCTTGGT
  661 GAAGGGGACC GCGTGGCGTG GAGCGCACAG TTGCTGGGGG AGCTTCGGCT CTGGGTGAAT
  721 GGGCGGGATT GCGTGTGCTG CACAGCTGCC CCCCTCGTGT CTGGGCGTCG TGACTTTATG
  781 CAGTGCACCA GATCACGTGC TACCCTGAGC AGCTCAGCCC CCTACTCCAT TCTAACTACT
  841 TCGAGCTTGC CCCACTGAGA CTCTGGCTTG CCTGACAGGA ACCTCTGCAG TGAGCTTCAT
  901 GGTGTCCCAG GCCAGGCCGT CGAACGTTCT AACGCTTGGA TCGGATTGAC GTGAGCACTG
961 GAACGCG
//
Primer: BGH_rev2
Sequence file: RDB13978_A6C2b.seq check
>D03172A6_A6C2_2_BGH_rev2_H01_22.ab1
    1 ACTGGCTAGG GGTAAGGGAA GGCAGAGGGG AGGGGCAAAC AACAGGATGG CTGGCAACTA
   61 GAAGGCACAG TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG
  121 CCCTCTAGAG CAGCCAACTC AGCTTCCTTT CGGGCTTTGT TAGCAGCCGG ATCTCAGTGG
  181 TGGTGGTGGT GGTGCTCGAA GATCTTGTCA TCGTCGTCCT TGTAGTCCTC GACCTCGACG
  241 CGGCCGCATT CCACCCGTAC CAGCAGGGCA CAGAGGAGTG TGGCCCCCTT CTCCTTAGTG
  301 ACCCACTCAA GTTCGGCTGG ACTGAAGTGA GGGTCAGGAG GTTCTCCAAG GGCAGCGGAA
  361 GGGGGTCCCG GGGTGTAGGA GCCAGGGCGC ACACACACCT GGAACGCCAC CTGAGCCTGG
  421 TGCGTCCGCT GGGATTTGGG GTCCCGGAAT TGCACTTTGG AGGCCAGGGT CTCCGCCCCA
  481 GCATATTGAA GGGAGGGGGA AAGCAGCACA GGAGGGGGCT GCTCCTCCCG AGGAGGTGCA
  541 CAGTTCTCGC CAGGCTCCTC GAACCCTACC CCAGGTTCTC CCTTCAAAGG ACGGCAGCTC
  601 AGGATGGAGG CAGTACCTGC TCCCAGCTCC CCTCGGTCCA GCACTCTCCG TACAGCGGCA
  661 ACATTGCTCC CGTGATATGC CATGTGCCAC TTCTTGGTTA GTGTCCCAGC CTCCAGGCGG
  721 GGATTCACTC TGAGGTTGAA CCTGCACCAG CCAAAGGGCA GTGCATATTC CCGGGGAGGC
  781 TCCCCTCTGC GCCTGTGGGC CTCGTCTCCT CGCAGCTTCC GGCAAGACTC ACAGTAGCAC
  841 AGGCTTCGCT TTGGCGGAGG CATGAAATAA TCTTCGGGAA GCAGCAGGAG TTCTTGGAAG
  901 CGAGAGCAAA GGGCATGGGT ACTCGCAGCT CTTTAGAGGA CTAGCGGCCA GTGGGTGGAC
  961 CCCAGCACAC ACTCTCTTTG ATACCGTCCA CCATGTCTGC TTTCTCCATG TCCCCTTTGG
 1021 TTCCAGCACT TTTTCCCACT GGCAGCCCCT GCTTCAAGGA ATCACGATTG TCAACTTGCT
1081 TCAACTACCA TAGAAGGTTC ACAAGCGCAA TGCATGGCCT TGGGGGGC
//
Primer: SV40pro_F
Sequence file: RDB13978_A6C2c.seq check
>D03172A6_A6C2_2_SV40pro_F_G12_21.ab1
    1 CCTGGTCTGG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC CTTCTATCGC
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTTC GAAATGACCG ACCAAGCGAC
  961 GCCCCAACTG CCATCACGAG ATTTCGATTT CCACCGCCGC GTCTATGAAC GTGGGCTCGA
 1021 ATCGGTTTCG GACGGCGCCT GGATGATCTC AAGCGCGGGG AATCTCATGC TGAGTCATCG
1081 CCACCCACTT GGTGATGCAG CTTATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]