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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HERC2 (#RDB13974)

Expression vector of human HERC2.

Clone info. Expression vector of human HERC2.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Gene/insert name human HERC2 cDNA
Depositor|Developer Imai, Yuzuru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13974 pEF-HERC2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-HERC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13974).

Reference section:

Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13974_A6C2p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Sequence file: RDB13974_A6C2a.seq check
>D03172A2_A6C2_1_EF1a_C_F_E11_14.ab1
    1 TATGGGATTG CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
   61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGTCGAGAT GCCCTCTGAA
  121 TCTTTCTGTT TGGCTGCCCA GGCTCGCCTC GACTCCAAAT GGTTGAAAAC AGATATACAG
  181 CTTGCATTCA CAAGAGATGG GCTCTGTGGT CTGTGGAATG AAATGGTTAA AGATGGAGAA
  241 ATTGTATACA CTGGAACAGA ATCAACCCAG AACGGAGAGC TCCCTCCTAG AAAAGATGAT
  301 AGTGTCGAAC CAAGTGGAAC AAAGAAAGAA GATCTGAATG ACAAAGAGAA AAAAGATGAA
  361 GAAGAAACTC CTGCACCTAT ATATAGGGCC AAGTCAATTC TGGACAGCTG GGTATGGGGC
  421 AAGCAACCAG ATGTGAATGA ACTGAAGGAG TGTCTTTCTG TGCTGGTTAA AGAGCAGCAG
  481 GCCCTGGCCG TCCAGTCAGC CACCACCACC CTCTCAGCCC TGCGACTCAA GCAGAGGCTG
  541 GTGATCTTGG AGCGCTATTT CATTGCCTTG AATAGAACCG TTTTTCAGGA GAATGTCAAA
  601 GTTAAGTGGA AAAGCAGCGG TATTTCTCTG CCTCCTGTGG ACAAAAAAAG TTCCCGGCCT
  661 GCGGGCAAAG GTGTGGAGGG GCTCGCCAGA GTGGGATCCC GAGCGGCGCT GTCTTTTGCC
  721 TTTGCCTTCC TGCGCAGGGC CTGGCGATCA GGCGAGGATG CGGACCTCTG CAGTGAGCTG
  781 TTGCAGGAGT CCCTGGACGC CCTGCGAGCA CTTCCCGAGG CCTCGCTCTT TGACGAGAGC
  841 ACCGTGTCCT CTGTGTGGCT GGAGGTGGTG GAGAGAGCGA CCAGGTTTCC TCAGGTCCGT
  901 CGTGACGGGG GGATGTTCAC GGAACGCCAG CCACCAAAGG GCCAGGAAGC ATCCCCCCTG
  961 CAGACCAGCA CTTGGCCCTG GCCCATCCTT GCTGGGAGCT GGCTGTGCAG AGGAGGCACG
 1021 CTTGAGCCCA AATGTTTGTC TGCCCATTCC TTGTTTGTTT GCTTTCAGCT GTGGGGACAG
1081 CGGGGCCACA GGAGACCTGG ACTATGAAGC GATTCCGCCC CAGGGGCAAC CAAGT
//
Primer: Poly(A) region primer #0
Sequence file: RDB13974_A6C2b.seq check
>D03172A2_A6C2_1_GNPmixture_D01_10.ab1
    1 AGGAAAAATT CGGTGAAAAT TCAGTGTACA ATGTGACAGT GGAGGGGACA CTGGTGAGTG
   61 GGGGGTGAAG AGGTGGAGGG TGACAGTGGA GCTTCCTTTC ACACACAGGC CTGACATGAT
  121 TCCTCCTGTC TGCTCCCTCC CACATCCCCA GTCCCCGTCC AGCAAGGCAA ATGTCCAGGC
  181 ACCTGCCTGG CCCTAAAAAG AGTCACAGTC CTGACCCTCG TGGGGGCAGG GCAGGTGTCC
  241 CGAGGCCACC CAGAAAAACA GGAGACACGT CGGTGATGTT CGGGAGTCAA ACCATGTCCC
  301 AAAAGTCTTA AGAAGAAAAA CGAGATTCCC CAGCAAATTC GTGGGACCCC AGGGCCATGC
  361 CTCCCAGCTC TGGCCACCTC TGCTTGTCCA GGGGCCTCAC AGCCAAGGGG ATGCAGGGGC
  421 CGCTGTGCAG TCGGCTGAGG CCAGAGTGAG GGGTGCAAGG ACCCAGATGG GGAACACTGC
  481 TGTTTAAATA TTAAACAGGG ATTTCTTGTC TCCCACGTGG GAGACCTGAT ACTCTCAAGG
  541 GTCCCAGGTG GGGACCCTCA CTCTAGGCTT TACTTGTACA GGGCCGCTTA GTGTCCTGTT
  601 AAATAATCTT GTGTAGAGTC CGAAGCAAAG GAGTCGACAT CCTCGTTATC TGAATCGTCG
  661 CTGCTGTCGT CGGCGGCTGG CTCTCCTGTA AGTGCGATGC GAGCGTAGTC ATCTGTGTCT
  721 ATGGACTTGC AGAAGTGGAT GGCGTACTTG AGCTTCTCCT CCAGCACCTG CTTGCAGGAA
  781 TACCTGGGCA GCTTTCAGCA AGAAGAAACA GGTGTAGGAC TCAGGGAGGA AGTGGGTCTG
  841 GAGGGTTGTA TTTATCCAAA CACCTGGATG ACGAAAGTCT TCGGCCCCGG AAGTCGCCGA
  901 TGGGTCCTGG GCTACCCTCG TCCGGCCCCA GAACGAAAGC AAAAGAAAAA GAGAACGCTC
  961 TGTTGTTTGG AAAAAGGACA CCAATCACCT TCCCAAAAAT CAACTTGGAT TCAGCGGATT
 1021 GACGGAAAGG GCTTCGAATG GCCTTTTTAA TAGGTGGATC AACCCGAAAC TTTTGAAGAA
1081 AAGTAGCTAG ACCGGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]