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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pClneoMyc-LATS2 (#RDB13970)

Expression vector of Myc-tagged LATS2.

Clone info. Expression vector of Myc-tagged LATS2. cDNA fragment 443 to 3709nt of NM_014572.2 is cloned.
Comment Expression was confirmed by the depositor with immunoboting using anti-Myc antibody. Known difference (at least): A to G substitution at 523nt (Lys, synonym).
Vector backbone pCIneo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human LATS2 cDNA
Depositor|Developer Hata, Yutaka |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13970 pClneoMyc-LATS2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pClneoMyc-LATS2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13970).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13970_A6Btp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13970_A6Bta.seq check
>D03116A4_A6Bt_1_CMV_Forward_B07_04.ab1
    1 CTTCACTGCG ATCGCCCGCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT
   61 CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCACT AGAAGCTTTA TTGCGGTAGT
  121 TTATCACAGT TAAATTGCTA ACGCAGTCAG TGCTTCTGAC ACAACAGTCT CGAACTTAAG
  181 CTGCAGTGAC TCTCTTAAGG TAGCCTTGCA GAAGTTGGTC GTGAGGCACT GGGCAGGTAA
  241 GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG TCGAGACAGA
  301 GAAGACTCTT GCGTTTCTGA TAGGCACCTA TTGGTCTTAC TGACATCCAC TTTGCCTTTC
  361 TCTCCACAGG TGTCCACTCC CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ACTTAATACG
  421 ACTCACTATA GGCTAGACCC CCCAACATGG AGCAGAAGCT TATCAGCGAG GAGGACCTGC
  481 TCGAGGAATT CACGCGTATG AGGCCAAAGA CTTTTCCTGC CACGACTTAT TCTGGAAATA
  541 GCCGGCAGCG ACTGCAAGAG ATTCGTGAGG GGTTAAAGCA GCCATCCAAG TCTTCGGTTC
  601 AGGGGCTACC CGCAGGACCA AACAGTGACA CTTCCCTGGA TGCCAAAGTC CTGGGGAGCA
  661 AAGATGCCAC CAGGCAGCAG CAGCAGATGA GAGCCACCCC AAAGTTCGGA CCTTATCAGA
  721 AAGCCTTGAG GGAAATCAGA TATTCCTTGT TGCCTTTTGC TAATGAATCG GGCACCTCTG
  781 CAGCTGCAGA AGTGAACCGG CAAATGCTGC AGGAACTGGT GAACGCAGGA TGCGACCAGG
  841 AGATGGCTGG CCGAGCTCTC AAGCAGACTG GCAGCAGAGC ATCGAGGCCG CCCTGGAGTA
  901 CATCAGCAAG ATGGGCTACC TGGACCCGAG GAATGAGCAG ATTGTGCGGG TCATTAAGCA
  961 GACCTCCCCA GGAAAGGGGC TCATGCCAAC CCCAGTGACG CGAGCCCAGC TTCGACGACG
 1021 GCGATTCGGT TGCGTTCTTA CAACAGCTGA GCCGTACCCC TTACGAGACC AGCCTCCGCG
1081 CTGACGACCA CGCGCTGGAG AATGCGCGCC GGTACGGTGG AC
//
Primer: pAxCALNL_F1
Sequence file: RDB13970_A6Btb.seq check
>D03116A4_A6Bt_1_pAxCALNL_F1_C07_07.ab1
    1 ACATCATCTT ATCATGTCTG CTCGAGCATT AACCCTCACT AAAGGGAAGC GGCCGCCCGG
   61 GTCGACTACA CGTACACAGG CTGGCAGCCT TCAGTCTGAT CCACCAGATC AGAGCTTTCT
  121 AAATCTGAGC TCTCAGCCTG TGAAGCTTCT GCTCCTGAAG GCTTTGGGCA TCGAAAGGGG
  181 TAGCCATTGT CATCAAAGAA CCTTCGGAAG GTGAATTCGT AAAATGCGTG CTCAGGATGC
  241 TTGTTATTGG GCGAGGTGAG TGTGTCCCAG GCCTTGGTGC TACCTTCGCT GGCATCGTTC
  301 CAAGGGCTTT CTTCATCTAC GGGGTCGAAA TTCGAGGTGT CCATGGGGTG GCTGATGGTG
  361 GGAACGTAGG GGGCTGGCTG CTTCCGGATG TCACTGGAGA AGTCAATGGC GCTGAAGAAG
  421 GGGTGGGCCT TCAGGTCATC GGCCCCATTC CGCCCCAGGC GGTGGTCTGC GGAGCAGCAC
  481 AGCTTGGTGA TGAGGTCCCT GGCCTCAGGG CTCAGCTTCA CCTGGGCTGG AATGTGGAGC
  541 GTGTTCTCCC AGTTGATCAC CTTCAGCTGG GTTTCTGTGG GAGTAGGTGC CAAAAAGGGC
  601 GGCTGCCCCA CCAGCATCTC GAAGAGAATC ACTCCAACAC TCCACCAGTC ACAGAGTTGA
  661 GTGTACCCTT TGCGGAGGAG CACCTCGGGT GCGATGTAGT TTGGAGTCCC CACCAGTGAA
  721 TGTGCCAGGC ACCTCTGGTG CTGCTTCCGC GCCCTCTGCT CTAGGGTCTT CAGCCTGTCC
  781 CCACACCGAC AGTTAGACAC ATCATCCCAG AGGTCGCTGG GCTCCATGCT GTCCTGTCTG
  841 ACATGGCTCC CTTTCTGGTA ATATTTGGAA ATTGTGAGTC CACCTGAACC CAGTGCAGAG
  901 GCCGAAATCT GTGAGTTTAA TGTGACCATC CAGATCTATC AAAATGTATC AGCCTTGATG
  961 TCTCGGTGGA TGAAGCCCAT CTGTGGACAC TCTCATGATC AAGGTCAGCT CTGCGATGTA
 1021 GACCGGCAGT TGCTCAGCAG ACTCATCCGG ATCAGCAGGC TCATCATGTC CGACAGATGT
1081 AGTCATCCAA AGTACAGGCC GGATCATTGT CCTG
//
Primer: SV40pro_F_V2
Sequence file: RDB13970_A6Btc.seq check
>D03116A4_A6Bt_1_SV40pro_F_V2_D07_10.ab1
    1 GTGCACTTTT TGGAGCTAGG CTTTTGCAAA AGCTTGATTC TTCTGACACA ACAGTCTCGA
   61 ACTTAAGGCT AGAGCCACCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC
  121 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
  181 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC
  241 CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG
  301 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT
  361 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC
  421 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
  481 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA
  541 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT
  601 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC
  661 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT
  721 GGCGGACCGC TATCAGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
  781 GAATGGGCTG ACCGCTTCTC GTGCTTTACG GTATCGCCGC CTCCCGATCG CAGCGCATCG
  841 CTTCTATCGC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA TGAACGACAG
  901 CGACGCCACT GCATCACGAT GCGCATAAAA ATCTTATTTC ATACATCGTG TGTGCTTTTG
  961 GGTGATCGAT AGCGTAGGAT CCGTAGGTGC ACTCAGTACA ATTCGCTGAT GGCGCCTAGT
1021 AGCAGCTCGA ACTGCACGTA GACCTGACGC TATGAAGTTC CGATA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]