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pCIneoMyc-LATS1 (#RDB13969)

Expression vector of Myc-tagged LATS1.

Clone info. Expression vector of Myc-tagged LATS1.
Comment Expression was confirmed by the depositor with immunoboting using anti-Myc.
Vector backbone pCIneo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human LATS1 cDNA
Depositor|Developer Hata, Yutaka |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13969 pCIneoMyc-LATS1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCIneoMyc-LATS1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13969).

Reference section:

Kawano, S., Maruyama, J., Nagashima, S., Inami, K., Qiu, W., Iwasa, H., Nakagawa, K., Ishigami-Yuasa, M., Kagechika, H., Nishina, H., Hata, Y., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13969_A6Btp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13969_A6Bta.seq check
>D03116A3_3_A6Bt_CMV_Forward_G06_21.ab1
    1 CTTCACTGCG ATCGCCCGCC CCGTTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
   61 TCTATATAAG CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT ATTGCGGTAG
  121 TTTATCACAG TTAAATTGCT AACGCAGTCA GTGCTTCTGA CACAACAGTC TCGAACTTAA
  181 GCTGCAGTGA CTCTCTTAAG GTAGCCTTGC AGAAGTTGGT CGTGAGGCAC TGGGCAGGTA
  241 AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT GTCGAGACAG
  301 AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA CTTTGCCTTT
  361 CTCTCCACAG GTGTCCACTC CCAGTTCAAT TACAGCTCTT AAGGCTAGAG TACTTAATAC
  421 GACTCACTAT AGGCTAGACC CCCCAACATG GAGCAGAAGC TTATCAGCGA GGAGGACCTG
  481 CTCGAGGAAT TCACGCGTAT GAAGAGGAGT GAAAAGCCAG AAGGATATAG ACAAATGAGG
  541 CCTAAGACCT TTCCTGCCAG TAACTATACT GTCAGTAGCC GGCAAATGTT ACAAGAAATT
  601 CGGGAATCCC TTAGGAATTT ATCTAAACTA TCTGATGCTG CTAAGGCTGA GCATAACATG
  661 AGTAAAATGT CAACCGAAGA TCCTCGACAA GTCAGAAATC CACCCAAATT TGGGACGCAT
  721 CATAAAGCCT TGCAGGAAAT TCGAAACTCT CTGCTTCCAT TTGCAAATGA AACAAATTCT
  781 TCTCGGAGTA CTTCAGAAGT TAATCCACAA ATGCTTCAAG ACTTGCAAGC TGCTGGATTT
  841 GATGAGGATA TGGTTATACA AGCTCTTCAG AAAACTAACA ACAGAAGTAT AGAAGCAGCA
  901 ATTGAATTCA TTAGTAAAAT GAGTTACCCA AGATCCTCGA CGAGAGCAGA TGGCTGCAGC
  961 AGCTGCCAGA CTATATGCAG CATGAACACG GATGTGCAGC ATCAGTACGC AAACAGAGCT
 1021 GGAAGTCCTA AGATTCGTAG TCTCGAGCAT GGCGCACTAG AGAAAGTTGC TATCATCTGA
1081 ACTCGAACTC ATGAACCGAA TTGTTACGAG AC
//
Primer: pAxCALNL_F1
Sequence file: RDB13969_A6Btb.seq check
>D03116A3_3_A6Bt_pAxCALNL_F1_H06_24.ab1
    1 ATATATATCT TTATCATGTC TGCTCGAAGC ATTAACCCTC ACTAAAGGGA AGCGGCCGCG
   61 TCGACGGTAC CTCAAAACAC CTCGCATTTC AGGCCCTTTT ACAAATCCTC ATTACATTTA
  121 TTTACTAGTG TGTTAAACAT ATACTAGATC GCGATTTTTA ATCTCTGAGC CTGTGTTTTG
  181 ATCATCTTCA TCCGACTGCT GCTCTGAGCC TTGTGAATTA ATGTATTCAT ATTCAATAGG
  241 CTTCGGATAA TTATATGGGT AGCCATTGTC ATCAAAAAAC CTTCGGAAGG TAAATTCATA
  301 GAATGCATGT TCAGGATGCT TTCCATTTTT ATACCATCCA TTGAGAGTGT CATTTACATT
  361 TTCTTCCTCG TTATCATCAC TCCATAATTT ATCAGGATCA ACAGGATCAA AATTTGATGT
  421 ATCTGTTGGG TGTGTGATTT TAGGAATGTA TGAAGCAGAC TGCTGTCTCA GGTCACTGGA
  481 GAAGTCAATT GTTTTAAAAA ATGGATGAGC TTTTATTTCA TCAGCACCAT TCTTGCCTAA
  541 GCGATCTTCG GGTCCTCGGC AAAGTTTAAT AATAAGATCA GAAGCTTCAG GACTGAGTTT
  601 AGCTTGTGGT GGAATGTGAA GAGATGTTTG CCAGTTGATA ACCTTCATTT GTGTTTCTAA
  661 TGGTGTTTGT GCCAAGAAAG GAGGTTGTCC CACCAACATT TCAAAAAGAA TAACACCAAC
  721 ACTCCACCAA TCACACAACT GTGTGTATCC TGTTCGTAGC AACACTTCAG GTGCAATATA
  781 ATTGGGAGTC CCAACCAAAG AATGTGCTAG ACATCGCTGG TGCTGGCGTG CAGCTCTCCG
  841 CTCTAATGGC TTCAGTCTGT CTCCACATCG ACAGCTTGAG GGATCCCCCC CATTCATTAC
  901 TGAAATCCAT GCTATCTTGC CGTGGATGGT CACCACTCTG ATAGTACTTA GAATCGTGTG
  961 TCCATCTGAA GCCAGTGCAG AGGCCCAAAG TCAGTCAATT TAATATGACC ATCACGATCA
 1021 TCAAAATATT ATCAGTTTAA TATCTCTATG ATAAACCCAT TTTTATGACA CTTTCACTGC
 1081 ACAGGTAAGT TCCTGCCTAT GTAGATTCGT GCCAGACTTT CTTGGAAAGA ATTGCCCCAA
1141 TCTA
//
Primer: SV40pro_F_V2
Sequence file: RDB13969_A6Btc.seq check
>D03116A3_A6Bt_3_SV40pro_F_V2_A07_01.ab1
    1 GTTGCCCCTT TTTTTGGAGG CTAGGCTTTT GCAAAAGCTT GATTCTTCTG ACACAACAGT
   61 CTCGAACTTA AGGCTAGAGC CACCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG
  121 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
  181 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
  241 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
  301 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
  361 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
  421 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
  481 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
  541 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
  601 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC
  661 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG
  721 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT
  781 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG
  841 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA
  901 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT
  961 TTCATTACAT CTGTGTGTTG GGTTTTTTTG TGTGAATCGA TAGCGATAAG GATCCCGCGT
 1021 ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCCGCATA GTTTAAGCAG CCCCGACCAC
 1081 CCGCCAACAC CGCTGAACGC GGCCCTGACC AGGCCTTGTC TGCCTTCCCC CGGGCAATTC
1141 CCGCGCCCGT TA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969. [link to RRC of NBRP]