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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pENTR4XE126 (#RDB13954)

Gateway Entry vector of human CSAP.

Clone info. Gateway Entry vector of human CSAP.
Vector backbone pENTR4 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Kanamycin^r
Gene/insert name Human CSAP cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Ohta, S., PLos One 10 (11): e0142798 2015.)
Remarks
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohta, S., PLos One 10 (11): e0142798 2015.)。
備考
Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13954 pENTR4XE126 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pENTR4XE126 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13954).

Reference section:

Ohta, S., Hamada, M., Sato, N., Toramoto, I., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13954_A6Bip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pA3'Element Reverse
Sequence file: RDB13954_A6Bia.seq check
>D03129A3_A6Bi_1_SV40pA3'element_Reverse_H10_22.ab1
    1 GCGGTCAGGG GGGGGCGGAG CCTATGGAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCTAGCA TGGATCTCGG GGACGTCTAA CTACTAAGCG AGAGTAGGGA
  181 ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGGAAG ACTGGGCCTT TCGTTTTATC
  241 TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC
  301 GTTGTGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT
  361 CAAACTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTCCT
  421 GTTAGTTAGT TACTTAAGCT CGGGCCCCAA ATAATGATTT TATTTTGACT GATAGTGACC
  481 TGTTCGTTGC AACAAATTGA TAAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA
  541 GCAGGCTCCA CCATGGGAAC CATGTCCCCG GGGAGCGGGG TGAAGAGCGA GTACATGAAG
  601 CGCTACCAGG AGCCGCGCTG GGAGGAGTAC GGGCCGTGCT ACCGCGAGCT GCTGCACTAC
  661 CGCCTAGGCC GCCGGCTGCT GGAGCAGGCG CACGCGCCCT GGCTCTGGGA CGACTGGGGC
  721 CCGGCCGGCT CCTCGGAGGG ACTCGGCGTC GTCAGAGTCG TCGGGGCGCC GGGGGGCCCC
  781 GCACCCCCGG TGCGCCCCCG CCCCTCGCCC CCGCCGCCCG GTAGAGCCGG CGACCCCAAG
  841 GAAGGAGGCG GAACGGGCGG GGCGCGCGGG GGCCCCCGGA AGGAGCAGGA ACGCCGAGGC
  901 CGGGGGGACG CCGGAAGCCC GAGGAAGCCG GAGGAACCCG GCTCTGCCAG CACTGGCCAG
  961 TGAAAAGAAT GATGAAAGAA TAACCTTGAA CAACCAAACC AAGAACAGAG AGACTTGACA
 1021 AATTCCCCCA CCAGTTCTTG GAGCCTTCGA CAGGAAACCA AAGTGGCTTT ATTTTGGCTA
 1081 GAATGGAAGA CAGGGAAAAG CCGGCTACGA AAGTCCGCCA ATAGAATCCA TGCGTAGGTC
1141 ACGAACATGA AGAAGGGAAA AACCCAAGGA CCATGCCCCA GAATGT
//
Primer: pDONR_R
Sequence file: RDB13954_A6Bib.seq check
>D03129A3_A6Bi_1_pDONR_R_A11_02.ab1
    1 GCCGGACGTC ACCTGGATGG CAATAATGAT TTTATTTTGA CTGATAGTGA CCTGTTCGTT
   61 GCAACAAATT GATAAGCAAT GCTTTCTTAT AATGCCAACT TTGTACAAGA AAGCTGGGTC
  121 TAGATAAGCT CTTGCCGAAT AGCACCTCAT GTATTCTGTC ATCCATGGGT TCTCTGAGGA
  181 GGAAGCCTTC ATCTTTCTGT TCTTCTCCAC ATCCACAGAG TGAGCTCGCT GCCTTTGAGC
  241 AACCAGTTTC CTTTTTTCCA CCTGTCTTCT GTTCTTGGCT CGTAATGCTG ATTCATGAAT
  301 CTCGTGCACA GGAGCGGACG CACAGACGTT GTGAGTCTTC TGGCTTCCTG TATCGGTCTG
  361 TTTTTCTCCC CAGCCATAAA GAGCAAATGG ATGCTTGTTT TCTTTTATTT TACTCGATGA
  421 TCTTTGGGGA CTTTTGACCG CTTTCCTGTT TCCTCTAGCA AATAAGGCAC TTGGTTGCTG
  481 TCGAGGCTCA GTACTGGTGG GTGATTTGTC AGTCTCTCTT GTTCTGGTTT GTTGTTCAGG
  541 TTTATCTTCT ACATCTTTCA CTGGCAGTGC TGGCAGAGCC GCGTCCTCCG CGTCCTCGGC
  601 CTCCGCGTCC CCGGCCTCCG CGTCCTGCTC CTCCGGGGCC CCGCGCGCCC GCCGTTCCGC
  661 CTCCTCCTGG GTCGCCGGCT CTACGGGCGG CGGGGGCGAG GGCGGGGCGC ACCGGGGTGC
  721 GGGGCCCCCG GCGCCCGACG ACTCTGACGA CGCCGAGTCC TCCGAGGAGC CGGCCGGGCC
  781 CCAGTCGTCC CAGAGCCAGG GCGCGTGCGC CTGCTCCAGC AGCCGGCGGC CTAGCGGTAG
  841 TGCAGCAGCT CGCGGTAGCA CGGCCCGTAC TCCTCCCAGC GCGGCTCCTG GTAGCGCTTC
  901 ATGTACTCGC TCTTCACCCC GCTCCCCGGG GACATGGTTC CCATGGTGGA GCCTGCTTTT
  961 TTGTACAAGT TGGCATTATA AAAAAGCATT GCTATCAATT GTTGCACGAA CAGTCACTAA
 1021 TCAGTCAAAA TAAAATCATT ATTTGGGGCC CGAGCTAGTT ACTTACTTAA CAGAGAGTTG
 1081 GTAGAAACGC AAAGCAATCG TCAGATGGCC TCTGGCTAGT GGAATGCTGC AGATTTATGC
1141 CAGACGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]