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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pDEST132NTrAP126 (#RDB13953)

Expression vector of human CSAP, TrAP tag.

Clone info. Expression vector of human CSAP, TrAP tag (his6, Streptavidin binding peptide, and S tag).
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 7.8 kb
Selectable markers Ampicillin^r (E. coli), Neomycin^r (mammalian cell)
Gene/insert name Human CSAP cDNA
Depositor|Developer Ohta, Shinya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Ohta, S., PLos One 10 (11): e0142798 2015.)
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohta, S., PLos One 10 (11): e0142798 2015.)。
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13953 pDEST132NTrAP126 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDEST132NTrAP126 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13953).

Reference section:

Ohta, S., Hamada, M., Sato, N., Toramoto, I., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13953_A6Bip1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13953_A6Bia.seq check
>D03129A2_A6Bi_1_CMV_Forward_E10_13.ab1
    1 TGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGCA TGCATCACCA TCACCATCAC ATGGACGAGA
  181 AGACCACCGG CTGGCGGGGC GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC
  241 TGCGGGCCAG GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCGGCGGC GGCGGCAAAG
  301 AAACCGCTGC TGCTAAATTC GAACGCCAGC ACATGGACAG CGGATCTCGA GCTCTGGAAG
  361 TTCTGTTCCA GGGGCCCGAA AACCTGTATT TTCAGGGTAC CACAAGTTTG TACAAAAAAG
  421 CAGGCTCCAC CATGGGAACC ATGTCCCCGG GGAGCGGGGT GAAGAGCGAG TACATGAAGC
  481 GCTACCAGGA GCCGCGCTGG GAGGAGTACG GGCCGTGCTA CCGCGAGCTG CTGCACTACC
  541 GCCTAGGCCG CCGGCTGCTG GAGCAGGCGC ACGCGCCCTG GCTCTGGGAC GACTGGGGCC
  601 CGGCCGGCTC CTCGGAGGAC TCGGCGTCGT CAGAGTCGTC GGGCGCCGGG GGCCCCGCAC
  661 CCCGGTGCGC CCCGCCCTCG CCCCCGCCGC CCGTAGAGCC GGCGACCCAG GAGGAGGCGG
  721 AACGGCGGGC GCGCGGGGCC CCGGAGGAGC AGGACGCGGA GGCCGGGGAC GCGGAGGCCG
  781 AGGACGCGGA GGACGCGGCT CTGCCAGCAC TGCCAGTGAA AGATGTAGAA GATAAACCTG
  841 AACAACAAAC CAGAACAAGA GAGACTGACA AATCACCCAC CAGTACTGAG CCTCGACAGC
  901 AACCAAGTGC CTTATTTGCT AGAGGAAACA GGAAAGCGGT CAAAAGTCCC CAAAGATCAT
  961 CGAGTAAAAT AAAAGAAAAC AAGCATCCAT TTGCTCTTTT ATGGCTGGGG AGAAAAACAG
 1021 ACCCGATACA GAAGCAGAGA CTCACACGTC TGTGCGTCCG CTCTGTGCAC GAGATCATGA
1081 TCAGCATACG AGCAGGACAG GAGGACAGCT GAAAAAATGG AAACCTTGGC TGGTC
//
Primer: BGH_rev2
Sequence file: RDB13953_A6Bib.seq check
>D03129A2_A6Bi_1_BGH_rev2_F10_16.ab1
    1 CGGTTAGGTA GAGCACGGGG GAGGGGCAAC AACAGATGGC TGGCAACTAG AAGGCACAGT
   61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTAC TTTAGACCAC TTTGTACAAG
  121 AAAGCTGGGT CTAGATAAGC TCTTGCCGAA TAGCACCTCA TGTATTCTGT CATCCATGGG
  181 TTCTCTGAGG AGGAAGCCTT CATCTTTCTG TTCTTCTCCA CATCCACAGA GTGAGCTCGC
  241 TGCCTTTGAG CAACCAGTTT CCTTTTTTCC ACCTGTCTTC TGTTCTTGGC TCGTAATGCT
  301 GATTCATGAA TCTCGTGCAC AGGAGCGGAC GCACAGACGT TGTGAGTCTT CTGGCTTCCT
  361 GTATCGGTCT GTTTTTCTCC CCAGCCATAA AGAGCAAATG GATGCTTGTT TTCTTTTATT
  421 TTACTCGATG ATCTTTGGGG ACTTTTGACC GCTTTCCTGT TTCCTCTAGC AAATAAGGCA
  481 CTTGGTTGCT GTCGAGGCTC AGTACTGGTG GGTGATTTGT CAGTCTCTCT TGTTCTGGTT
  541 TGTTGTTCAG GTTTATCTTC TACATCTTTC ACTGGCAGTG CTGGCAGAGC CGCGTCCTCC
  601 GCGTCCTCGG CCTCCGCGTC CCCGGCCTCC GCGTCCTGCT CCTCCGGGGC CCCGCGCGCC
  661 CGCCGTTCCG CCTCCTCCTG GGTCGCCGGC TCTACGGGCG GCGGGGGCGA GGGCGGGGCG
  721 CACCGGGGTG CGGGGCCCCC GGCGCCCGAC GACTCTGACG ACGCCGAGTC CTCCGAGGAG
  781 CCGGCCGGGC CCCAGTCGTC CCAGAGCCAG GGCGCGTGCG CCTGCTCCAG CAGCCGGCGG
  841 CCTAGGCGGT AGTGCAGCAG CTCGCGGTAG CACGGCCCGT ACTCCTCCCA GCGCGGCTCC
  901 TGGTAGCGCT TCATGTACTC GCTCTTCACC CCGCTCCCCG GGGACATGGT TCCCATGGTG
  961 GAGCCTGCTT TTTTGTACAA ACTTGTGGGT ACCCTGAAAA TACAGGTTTT CGGCCCCTGG
 1021 AACAGAACTT TCCAGAGCTC GAGATCCGCT GTCCATGTGC TGGCGTTCGA ATTAGCAGCA
 1081 GCGGCTTCTT GCCGCCGCCG CGGCTCCCGC TGCCTGACGT GGTGCTTCAG GCCTGGACTG
1141 CAGCTGACTC AGCTCGATCG GAATCA
//
Primer: pAxEF_R2
Sequence file: RDB13953_A6Bic.seq check
>D03129A2_A6Bi_1_pAxEF_R2_G10_19.ab1
    1 GGGGGAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTGGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG
  121 ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG
  181 GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC
  241 AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG
  301 GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG
  361 CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC
  421 AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC
  481 CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCGCGC CTTGAGCCTG GCGAACAGTT
  541 CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT
  601 CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG
  661 CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG
  721 GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC
  781 TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC
  841 ACGATAGCCG CGCTGCCTCG TCCTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA
  901 CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA
  961 TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCCTCTCCA CCCCAAGCGG CCCGGAGACC
 1021 CTGCGTGCAT CCCATCTTGT TCAATCATGC GAACGATCCT CATCTGTCTC TGATCAGATC
 1081 CGAAAATGCA TTACCAGCTT CCGGAAGCTT TTTGGCAAAA GCCGTAGGGC CCTTCCAACA
1141 T
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohta, S., Polyglutamylated Tubulin Binding Protein C1orf96/CSAP Is Involved in Microtubule Stabilization in Mitotic Spindles. PLoS One 10 (11): e0142798, 2015. PMID 26562023. [link to RRC of NBRP]