A Genome editting tool for confirm the activity of CRISPR.
| Clone info. | A Genome editting tool for confirm the activity of CRISPR. |
|---|---|
| Vector backbone | p2color (Plasmid) |
| Size of vector backbone | 8.4 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cells). |
| Growth conditions | LB, Ampicillin, 37oC |
| Depositor|Developer | Sugiyama, Fumihiro | Mizuno, Seiya | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment In order to receive this biological resource, please use the Exclusive MTA [Link]. Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hasegawa, Y., Exp. Anim. 65 (3): 319-327 (2016)). |
[open/close]| 必要書類 | 提供依頼書 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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| MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hasegawa, Y., Exp. Anim. 65 (3): 319-327 (2016))。 |
| Catalog # | Resource name | Availability | Shipping form | Fee |
|---|---|---|---|---|
| RDB13948 | p2color | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
| The p2color was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13948). |
Reference section:
| Hasegawa, Y., Hoshino, Y., Ibrahim, A.E., Kato, K., Daitoku, Y., Tanimoto, Y., Ikeda, Y., Oishi, H., Takahashi, S., Yoshiki, A., Yagami, K., Iseki, H., Mizuno, S., Sugiyama, F., Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp. Anim. 65 (3): 319-327 (2016). PMID 27053096. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
| original | Hasegawa, Y., Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp. Anim. 65 (3): 319-327 (2016). PMID 27053096. [PubMed] [Article] [RRC of NBRP] |
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| reference | Ieda, D., Two mouse models carrying truncating mutations in Magel2 show distinct phenotypes. PLoS One 15 (8): e0237814 (2020). PMID 32804975. [PubMed] [Article] |
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