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px330-Ins1

Expression vector for hCas9 and guide RNA of mouse Ins1 gene.

Catalog number RDB13945
Resource name px330-Ins1
Clone info. Expression vector for hCas9 and guide RNA of mouse Ins1 gene.
Comment Function was confirmed by the depositor by 2color assay.
Vector backbone pX330 (Plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name mouse Ins1 genomic DNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
Other clones in our bank
External Database
mouse Ins1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Hasegawa, Y., Exp. Anim. 65 (3): 319-327 (2016)).
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hasegawa, Y., Exp. Anim. 65 (3): 319-327 (2016))。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13945 px330-Ins1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Hasegawa, Y., Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp. Anim. 65 (3): 319-327 (2016). PMID 27053096.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Hasegawa, Y., Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp. Anim. 65 (3): 319-327 (2016). PMID 27053096.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl