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pCAG-EGxxFP-Tyr-M

A simple validation system for the mouse Tyr genomic region targeted double strand breaks.

Catalog number RDB13944
Resource name pCAG-EGxxFP-Tyr-M
Clone info. A simple validation system for the mouse Tyr genomic region targeted double strand breaks by observing GFP reconstituted.
Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pCAG-EGxxFP (Plasmid)
Size of vector backbone 6.9 kb
Selectable markers Amp^r
Gene/insert name mouse Tyr genomic DNA Aequorea victoria GFP cDNA
Depositor Sugiyama, Fumihiro | Mizuno, Seiya |
 
Other clones in our bank mouse Tyr (NCBI Gene 22173) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(MAMM GENOME. 25 (7-8):327-34 ,2014)
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13944 pCAG-EGxxFP-Tyr-M Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

2019.07.20

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