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pCAG-EGxxFP-Tyr-M

A simple validation system for the mouse Tyr genomic region targeted double strand breaks.

Catalog number RDB13944
Resource name pCAG-EGxxFP-Tyr-M
Clone info. A simple validation system for the mouse Tyr genomic region targeted double strand breaks by observing GFP reconstituted.
Comment Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pCAG-EGxxFP (Plasmid)
Size of vector backbone 6.9 kb
Selectable markers Amp^r
Gene/insert name mouse Tyr genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
Other clones in our bank

External Database
mouse Tyr Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(MAMM GENOME. 25 (7-8):327-34 ,2014)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(MAMM GENOME. 25 (7-8):327-34 ,2014)
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13944 pCAG-EGxxFP-Tyr-M Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

          2022.05.17

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