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px330-Tyr-M

Expression vector for hCas9 and guide RNA of mouse Tyr gene.

Catalog number RDB13943
Resource name px330-Tyr-M
Clone info. Expression vector for hCas9 and guide RNA of mouse Tyr gene.
Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pX330 (Plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r
Gene/insert name mouse Tyr genomic DNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
 
Other clones in our bank mouse Tyr (NCBI Gene 22173) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(MAMM GENOME. 25 (7-8):327-34 ,2014)
Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please refer the Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose).
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13943 px330-Tyr-M Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

Further references such as user reports and related articles (go to bottom)

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Sequence information

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Mizuno, S., Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm. Genome. 25 (7-8): 327-334 (2014). PMID 24879364.

2021.03.11

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