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pCAG-EGxxFP-NE

A simple validation system for the mouse Kit WE genomic region targeted double strand breaks.

Catalog number RDB13942
Resource name pCAG-EGxxFP-NE
Clone info. A simple validation system for the mouse Kit WE genomic region targeted double strand breaks by observing GFP reconstituted.
Comment Mouse genomic region near the Exoc1 gene (Chr. 5: 76,443,899–76,444,843 of NC_000071.6). Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pCAG-EGxxFP (Plasmid)
Size of vector backbone 7.3 kb
Selectable markers Amp^r
Gene/insert name mouse Exoc1 genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
Other clones in our bank

External Database
mouse Exoc1 Aequorea victoria GFP

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Sci Rep. 5:13632, 2015.)
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep. 5:13632, 2015.)
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13942 pCAG-EGxxFP-NE Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Mizuno, S., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Mizuno, S., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620.

          2023.04.10

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