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pCAG-EGxxFP-PK

A simple validation system for the mouse Kit WE genomic region targeted double strand breaks.

Catalog number RDB13941
Resource name pCAG-EGxxFP-PK
Clone info. A simple validation system for the mouse Kit WE genomic region targeted double strand breaks by observing GFP reconstituted.
Comment Mouse genomic region near the Kit gene (Chr. 5: 75,408,224–7,5408,767 of NC_000071.6). Function was confirmed by the depositor by EGxxFP assay.
Vector backbone pCAG-EGxxFP (Plasmid)
Size of vector backbone 6.9 kb
Selectable markers Amp^r
Gene/insert name mouse Kit genomic DNA Aequorea victoria GFP cDNA
Depositor|Developer Sugiyama, Fumihiro | Mizuno, Seiya |
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External Database
mouse Kit Aequorea victoria GFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Sci Rep. 5:13632, 2015.)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep. 5:13632, 2015.)
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB13941 pCAG-EGxxFP-PK Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Mizuno, S., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Mizuno, S., Peri-implantation lethality in mice carrying megabase-scale deletion on 5qc3.3 is caused by Exoc1 null mutation. Sci. Rep. 5: 13632 (2015). PMID 26346620.

          2022.05.17

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