Sequencing and PCR primers for primer information.
Expression vector of GST-tagged ncNLS (ASQGTKRSYEQMET).
| Catalog number | RDB13757 |
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| Resource name | pGEX-NP-ncNLS |
| Clone info. | Expression vector of GST-tagged influenza virus ncNLS (ASQGTKRSYEQMET). A non-classical nuclear localization signal of influenza A virus nucleoprotein. Expression was confirmed by the depositor with SDS-PAGE and crystallization. Refer Matsuura, Y. Nature 432 (7019): 872-877, 2004, PMID:15602554 for pGEX-TEV vector. |
| Vector backbone | pGEX-TEV (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Influenza virus nuclear protein NP cDNA |
| Depositor | Matsuura, Yoshiyuki | |
| Other clones in our bank | Influenza virus NP | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakada, R., Sci. Rep. 5: 15055 (2015)) (2) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB13757 | pGEX-NP-ncNLS | DNA solution |
Electronic file
Featured content
References
| original | Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13757_A6Acp1-2.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: pGEX_R | RDB13757_A6Aca.seq |
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>D03041A1_A6Ac_7_pGEX_R_E05_14.ab1 1 TTCATACGTA CTCGCGAGGC AGATCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTTAAGT 61 CTCCATTTGT TCATAAGAAC GTTTGGTGCC TTGAGACGCG GATCCCTGGA AATACAAGTT 121 CTCCGTGGTC GGGATGTCAT AATCAGATCC ATCCGGAACC AGATCCGATT TTGGAGGATG 181 GTCGCCACCA CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA 241 TTTCAAGTAC TTATCAATTT GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT 301 TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA 361 CATGAAGTCA GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC 421 TTCGAACATT TTCAGCATTT CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA 481 GTCTTTACTA TATGCAATTC TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG 541 CATTGAAATC TCTGCACGCT CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT 601 ATAACGTATG ATGGCCATAG ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG 661 ATTGGGAAAC TCCAAACCCA ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG 721 CTCATACAAA TGCTCTTCAT ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG 781 CACAAGGCCC TTAATTTTCC AATAACCTAG TATAGGGGAC ATGAATACTG TTTCCTGTGT 841 GAAATTGTTA TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG 901 CTCATTTCAG AATATTTGCC AGAACCCGTT ATGATGTCGG CGCAAAAAAC ATTATCCAGA 961 ACGGGAGTGC GCCTTTGAGC GACACGAATT ATGCAGTGAT TTACGACTGC ACAGCATACC 1021 ACAGCTTTCC GATGCCTGGC TGACGCAGAA GCATTGGTGC ACGTGCAGTT CGATTAGGCT 1081 AATTCAACGT CATGAAGATA ATTCGCGTCT GGCCTTCCTG TTAAGCC // |
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| Primer: M13(-40) | RDB13757_A6Acb.seq |
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>D03041A1_A6Ac_7_M13(-40)_F05_17.ab1 1 CCGGCAAGGA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA 61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG 121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG 181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC 241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG 301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG 361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC 421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG 481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG 541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG 601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA 661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC 721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA 781 GACATCAAGA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG 841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGGCAC 901 CGCCGCTTTA CAGGGCTTCG AACGCCGGCT TTCGTTCTAC CAATCGAACA CAACGCTGGC 961 ACAAGTTGGA TCCGGGCGCG GAGATTTATT CGGCTGCGAC AATTGCGACG GCCGTTGCAA 1021 GGCCAGACTT GAGGTGGCAA CGGCAATCAG CACGACTGTT TGGCCCGCCA AGCTTGTTTG 1081 CCACCGGTTG GGAGTATCAC GTCGGGACC // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934. |
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2019.03.15
GNP_filter3_RDBDEP_html_190307.pl