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pGEX-NP-ncNLS (#RDB13757)

Expression vector of GST-tagged ncNLS (ASQGTKRSYEQMET).

Clone info. Expression vector of GST-tagged influenza virus ncNLS (ASQGTKRSYEQMET).
Comment A non-classical nuclear localization signal of influenza A virus nucleoprotein. Expression was confirmed by the depositor with SDS-PAGE and crystallization. Refer Matsuura, Y. Nature 432 (7019): 872-877, 2004, PMID:15602554 for pGEX-TEV vector.
Vector backbone pGEX-TEV (Plasmid)
Selectable markers Amp^r
Gene/insert name Influenza virus nuclear protein NP cDNA
Depositor|Developer Matsuura, Yoshiyuki |
 

Distribution information

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Terms and conditions for distribution (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakada, R., Sci. Rep. 5: 15055 (2015)). (2) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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MTAに書く使用条件 １．利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Nakada, R., Sci. Rep. 5: 15055 (2015)) ２．利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 ３．営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13757 pGEX-NP-ncNLS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-NP-ncNLS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13757).

Reference section:

Nakada, R., Hirano, H., Matsuura, Y., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13757_A6Acp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_R ()
Sequence file: RDB13757_A6Aca.seq check
>D03041A1_A6Ac_7_pGEX_R_E05_14.ab1
    1 TTCATACGTA CTCGCGAGGC AGATCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTTAAGT
   61 CTCCATTTGT TCATAAGAAC GTTTGGTGCC TTGAGACGCG GATCCCTGGA AATACAAGTT
  121 CTCCGTGGTC GGGATGTCAT AATCAGATCC ATCCGGAACC AGATCCGATT TTGGAGGATG
  181 GTCGCCACCA CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA
  241 TTTCAAGTAC TTATCAATTT GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT
  301 TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA
  361 CATGAAGTCA GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC
  421 TTCGAACATT TTCAGCATTT CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA
  481 GTCTTTACTA TATGCAATTC TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG
  541 CATTGAAATC TCTGCACGCT CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT
  601 ATAACGTATG ATGGCCATAG ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG
  661 ATTGGGAAAC TCCAAACCCA ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG
  721 CTCATACAAA TGCTCTTCAT ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG
  781 CACAAGGCCC TTAATTTTCC AATAACCTAG TATAGGGGAC ATGAATACTG TTTCCTGTGT
  841 GAAATTGTTA TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG
  901 CTCATTTCAG AATATTTGCC AGAACCCGTT ATGATGTCGG CGCAAAAAAC ATTATCCAGA
  961 ACGGGAGTGC GCCTTTGAGC GACACGAATT ATGCAGTGAT TTACGACTGC ACAGCATACC
 1021 ACAGCTTTCC GATGCCTGGC TGACGCAGAA GCATTGGTGC ACGTGCAGTT CGATTAGGCT
1081 AATTCAACGT CATGAAGATA ATTCGCGTCT GGCCTTCCTG TTAAGCC
//
Primer: M13(-40) ()
Sequence file: RDB13757_A6Acb.seq check
>D03041A1_A6Ac_7_M13(-40)_F05_17.ab1
    1 CCGGCAAGGA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
   61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
  121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
  841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGGCAC
  901 CGCCGCTTTA CAGGGCTTCG AACGCCGGCT TTCGTTCTAC CAATCGAACA CAACGCTGGC
  961 ACAAGTTGGA TCCGGGCGCG GAGATTTATT CGGCTGCGAC AATTGCGACG GCCGTTGCAA
 1021 GGCCAGACTT GAGGTGGCAA CGGCAATCAG CACGACTGTT TGGCCCGCCA AGCTTGTTTG
1081 CCACCGGTTG GGAGTATCAC GTCGGGACC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934. [link to RRC of NBRP]