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pGEX-NP-ncNLS

Expression vector of GST-tagged ncNLS (ASQGTKRSYEQMET).

Catalog number RDB13757
Resource name pGEX-NP-ncNLS
Clone info. Expression vector of GST-tagged influenza virus ncNLS (ASQGTKRSYEQMET).
A non-classical nuclear localization signal of influenza A virus nucleoprotein. Expression was confirmed by the depositor with SDS-PAGE and crystallization. Refer Matsuura, Y. Nature 432 (7019): 872-877, 2004, PMID:15602554 for pGEX-TEV vector.
Vector backbone pGEX-TEV (Plasmid)
Selectable markers Amp^r
Gene/insert name Influenza virus nuclear protein NP cDNA
Depositor Matsuura, Yoshiyuki |
 
Other clones in our bank Influenza virus NP |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakada, R., Sci. Rep. 5: 15055 (2015)) (2) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13757 pGEX-NP-ncNLS DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13757_A6Acp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX_R RDB13757_A6Aca.seq
>D03041A1_A6Ac_7_pGEX_R_E05_14.ab1
    1 TTCATACGTA CTCGCGAGGC AGATCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTTAAGT
   61 CTCCATTTGT TCATAAGAAC GTTTGGTGCC TTGAGACGCG GATCCCTGGA AATACAAGTT
  121 CTCCGTGGTC GGGATGTCAT AATCAGATCC ATCCGGAACC AGATCCGATT TTGGAGGATG
  181 GTCGCCACCA CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA
  241 TTTCAAGTAC TTATCAATTT GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT
  301 TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA
  361 CATGAAGTCA GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC
  421 TTCGAACATT TTCAGCATTT CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA
  481 GTCTTTACTA TATGCAATTC TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG
  541 CATTGAAATC TCTGCACGCT CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT
  601 ATAACGTATG ATGGCCATAG ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG
  661 ATTGGGAAAC TCCAAACCCA ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG
  721 CTCATACAAA TGCTCTTCAT ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG
  781 CACAAGGCCC TTAATTTTCC AATAACCTAG TATAGGGGAC ATGAATACTG TTTCCTGTGT
  841 GAAATTGTTA TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG
  901 CTCATTTCAG AATATTTGCC AGAACCCGTT ATGATGTCGG CGCAAAAAAC ATTATCCAGA
  961 ACGGGAGTGC GCCTTTGAGC GACACGAATT ATGCAGTGAT TTACGACTGC ACAGCATACC
 1021 ACAGCTTTCC GATGCCTGGC TGACGCAGAA GCATTGGTGC ACGTGCAGTT CGATTAGGCT
1081 AATTCAACGT CATGAAGATA ATTCGCGTCT GGCCTTCCTG TTAAGCC
//
Primer: M13(-40) RDB13757_A6Acb.seq
>D03041A1_A6Ac_7_M13(-40)_F05_17.ab1
    1 CCGGCAAGGA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
   61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
  121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
  181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
  241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
  301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
  361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
  421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
  481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
  541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
  601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
  661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
  721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
  781 GACATCAAGA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
  841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGGCAC
  901 CGCCGCTTTA CAGGGCTTCG AACGCCGGCT TTCGTTCTAC CAATCGAACA CAACGCTGGC
  961 ACAAGTTGGA TCCGGGCGCG GAGATTTATT CGGCTGCGAC AATTGCGACG GCCGTTGCAA
 1021 GGCCAGACTT GAGGTGGCAA CGGCAATCAG CACGACTGTT TGGCCCGCCA AGCTTGTTTG
1081 CCACCGGTTG GGAGTATCAC GTCGGGACC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934.

2019.03.15

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