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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form) (#RDB13752)

Expression vector of human eIF5A, unhypusinated form.

Clone info. Expression vector of FLAG- and His-tagged mutant eIF5A (K50A), which cannot be hypusinated.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pcDNA3.1 Directional TOPO (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name human eIF5A cDNA
Depositor|Developer Seko, Yoshinori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Seko, Y., Sci. Rep. 5: 13737 2015.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Seko, Y., Sci. Rep. 5: 13737 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13752 FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13752).

Reference section:

Seko, Y., Fujimura, T., Yao, T., Taka, H., Mineki, R., Okumura, K., Murayama, K., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13752_A6A5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13752_A6A5a.seq check
>D02955A3_A6A5_7_CMV_Forward_D09_12.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG ATCCAGTACC
  181 CTTCACCGAA TTCAAAATGG CAGATGACTT GGACTTCGAG ACAGGAGATG CAGGGGCCTC
  241 AGCCACCTTC CCAATGCAGT GCTCAGCATT ACGTAAGAAT GGCTTTGTGG TGCTCAAAGG
  301 CCGGCCATGT AAGATCGTCG AGATGTCTAC TTCGAAGACT GGCGCGCACG GCCACGCCAA
  361 GGTCCATCTG GTTGGTATTG ACATCTTTAC TGGGAAGAAA TATGAAGATA TCTGCCCGTC
  421 AACTCATAAT ATGGATGTCC CCAACATCAA AAGGAATGAC TTCCAGCTGA TTGGCATCCA
  481 GGATGGGTAC CTATCACTGC TCCAGGACAG CGGGGAGGTA CGAGAGGACC TTCGTCTCCC
  541 TGAGGGAGAC CTTGGCAAGG AGATTGAGCA GAAGTACGAC TGTGGAGAAG AGATCCTGAT
  601 CACGGTGCTG TCTGCCATGA CAGAGGAGGC AGCTGTTGCA ATCAAGGCCA TGGCAAAAGA
  661 TTACAAGGAC GACGATGACA AGCACCACCA TCACCATCAC TGACTCGAGT ATATAAGGGT
  721 CAAGACAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGCGG
  781 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
  841 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
  901 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCCTTCCTTG ACCCTGGAAA GTGCCACTCC
  961 CACTGTCCCT TTCCTAATAA AATGAGGAAA TTTGCATCGC ATTGTCTGAG TAGTGTCATT
 1021 CTATTCCTGG GGGGTTGGGG TGGGGGCAGA CAGCCAGGGG GAAGAATGGT AGACCATAGC
1081 AGCATGCTAC GATGCGTGGC TCTATGGCCT TCTGAGC
//
Primer: SV40pro_F
Sequence file: RDB13752_A6A5b.seq check
>D02955A3_A6A5_7_SV40pro_F_F09_18.ab1
    1 CCCTGTCTGG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAAACCT GCCCATCACG AGATTTCGAT TTCCACCGCC CGCTTTCTAT GAAACGTTGG
 1021 CTTTCGGAAT CGTTTTCTGG ACGGCGGCTG CATGGATCCT TCAGCGCGGG GATCTCATGC
1081 TGAGTCATGC TCACCCGAAC TGGTTTAATT GCAGGTCTTT ATTAATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]