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eIF5A cDNA on pcDNA4/Myc-His vector (#RDB13750)

Expression vector of human eIF5A.

Clone info. Expression vector of Myc- and His-tagged human eIF5A.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pcDNA4/Myc-His (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin (E. coli), Zeocin (mammalian cells)
Gene/insert name human eIF5A cDNA
Depositor|Developer Seko, Yoshinori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Seko, Y., Sci. Rep. 5: 13737 2015.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Seko, Y., Sci. Rep. 5: 13737 2015.)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13750 eIF5A cDNA on pcDNA4/Myc-His vector DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The eIF5A cDNA on pcDNA4/Myc-His vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13750).

Reference section:

Seko, Y., Fujimura, T., Yao, T., Taka, H., Mineki, R., Okumura, K., Murayama, K., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13750_A6A5p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13750_A6A5a.seq check
>D02955A1_A6A5_1_CMV_Forward_A04_01.ab1
    1 TCGTCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT
  181 CCAGTGTGGT GGAATTCAAA ATGGCAGATG ACTTGGACTT CGAGACAGGA GATGCAGGGG
  241 CCTCAGCCAC CTTCCCAATG CAGTGCTCAG CATTACGTAA GAATGGCTTT GTGGTGCTCA
  301 AAGGCCGGCC ATGTAAGATC GTCGAGATGT CTACTTCGAA GACTGGCAAG CACGGCCACG
  361 CCAAGGTCCA TCTGGTTGGT ATTGACATCT TTACTGGGAA GAAATATGAA GATATCTGCC
  421 CGTCAACTCA TAATATGGAT GTCCCCAACA TCAAAAGGAA TGACTTCCAG CTGATTGGCA
  481 TCCAGGATGG GTACCTATCA CTGCTCCAGG ACAGCGGGGA GGTACGAGAG GACCTTCGTC
  541 TCCCTGAGGG AGACCTTGGC AAGGAGATTG AGCAGAAGTA CGACTGTGGA GAAGAGATCC
  601 TGATCACGGT GCTGTCTGCC ATGACAGAGG AGGCAGCTGT TGCAATCAAG GCCATGGCAA
  661 AACTCGAGTC TAGAGGGCCC TTCGAACAAA AACTCATCTC AGAAGAGGAT CTGAATATGC
  721 ATACCGGTCA TCATCACCAT CACCATTGAG TTTAAACCCG CTGATCAGCC TCGACTGTGC
  781 CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG
  841 GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA
  901 GGTGTCATTC TATTCTGGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA
  961 GACAATAGCA GGCATGCTGG GGATGCGGTG GCTCTATGGC TTCTGAGCGG AAAGAACAGC
 1021 TGGGGCTCTA GGGGTATCCC ACGCGCCCTG TAGCGGCGCA TAGCGCGCCG GTGTGTGCTA
1081 ACGCGCAGCT GACGCTACCG TGCAGCGACT TAGCACCGCC CTCGATTTCG CTTTCC
//
Primer: SV40pro_F
Sequence file: RDB13750_A6A5b.seq check
>D02955A1_A6A5_1_sv40pro_F_C04_07.ab1
    1 CCTCCGTCCT GTAGCAATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
  121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
  181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
  241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
  301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
  361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
  421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
  481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
  541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
  601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
  661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
  721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
  781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
  841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
  901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTTGGGGGT GCCTAATGAG TGAGCTAACT
  961 CACATTTATT TGCGTTGCGC TCACTGCCCG CTTTTCCAGT CGGGAAACCT GTCGTGCCAG
 1021 CTGCATTAAA TGAAATCGGC CAACGCGCGG GGGAGAGGGC AGATTTGCGT AATTGGGGGC
 1081 GCTCTTCGGT TCCTTCGCTC ACTGAACTCC GCTGCGCTCG GATCGCTCGG CTTGCGGCGG
1141 AGCGGTATCC AGCTCCACTT CAAAAGGCGC GGGTTAAATA TCCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594. [link to RRC of NBRP]