Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLEX_ALDH2Glu504Lys

Lentiviral plasmid vector of FLAG-tagged mutant ALDH2

Catalog number RDB13742
Resource name pLEX_ALDH2Glu504Lys
Clone info. Lentiviral plasmid vector of FLAG-tagged mutant ALDH2 (Glu504Lys). This point mutation causes catalytic deficiency of human ALDH2.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pLEX-MCS (plasmid)
Size of vector backbone 10.7 kb
Selectable markers Ampicillin, Zeocin (E. coli), Puromycin (mammalian cells).
Gene/insert name human ALDH2 cDNA
Depositor|Developer Muto, Manabu |
Other clones in our bank

External Database
human ALDH2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13742 pLEX_ALDH2Glu504Lys Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Amanuma, Y., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13742_A6A5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13742_A6A5a.seq check
          >D02971A3_A6A5_1_CMV_Forward_B02_05.ab1
              1 CGGGTTAACT CCGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
            121 CCTCCATAGA AGACACCGAC TCTACTAGAG GATCCACTAG CGATTACGCC AAGCTCGAAA
            181 TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTCCACCG CGGTGGCGGC CGCCACCATG
            241 GATTACAAGG ATGACGACGA TAAGAGCCCG GGCGGATCCC CCGGGCTGCA GGAATTCATG
            301 TTGCGCGCTG CCGCCCGCTT CGGGCCCCGC CTGGGCCGCC GCCTCTTGTC AGCCGCCGCC
            361 ACCCAGGCCG TGCCTGCCCC CAACCAGCAG CCCGAGGTCT TCTGCAACCA GATTTTCATA
            421 AACAATGAAT GGCACGATGC CGTCAGCAGG AAAACATTCC CCACCGTCAA TCCGTCCACT
            481 GGAGAGGTCA TCTGTCAGGT AGCTGAAGGG GACAAGGAAG ATGTGGACAA GGCAGTGAAG
            541 GCCGCCCGGG CCGCCTTCCA GCTGGGCTCA CCTTGGCGCC GCATGGACGC ATCACACAGG
            601 GGCCGGCTGC TGAACCGCCT GGCCGATCTG ATCGAGCGGG ACCGGACCTA CCTGGCGGCC
            661 TTGGAGACCC TGGACAATGG CAAGCCCTAT GTCATCTCCT ACCTGGTGGA TTTGGACATG
            721 GTCCTCAAAT GTCTCCGGTA TTATGCCGGC TGGGCTGATA AGTACCACGG GAAAACCATC
            781 CCCATTGACG GAGACTTCTT CAGCTACACA CGCCATGAAC CTGTGGGGGT GTGCGGGCAG
            841 ATCATTCCGT GGAATTTCCC GCTCCTGATG CAAGCATGGA AGCTGGGCCC AGCCTTGGCA
            901 ACTGGAAACG TGGTTGTGAT GAAGGTAGCT GAGCAGACAC CCCCTCACCG CCCTCTATGT
            961 GGCCCAACCC TGATCAAGGA GGCTGGCTTT TCCCCCCTGG TGTGGTCAAC ATTTGTGCCC
           1021 TGCATTTGGC CCCCCACGGC TGGGGGCGGC CCATTGCCTC CCATGGAGGA ATGTGATAAA
          1081 GTGGCAATTC CACCAAGGAC TTCCAACA
          //
          Primer: IRES-R
          Sequence file: RDB13742_A6A5b.seq check
          >D02971A3_A6A5_1__IRES_R_C06_09.ab1
              1 GAATATCGGG GCTTTGGGCC AGTACGTTTA GGGGGGGGGG AGGGGAGAGG GGGCGACCGG
             61 TGGCCAGACG CGTCGGGCCC TCTAGACTCG AGTTATGAGT TCTTCTGAGG CACTTTGACT
            121 GTGACAGTTT TCACTTTAGT GTATGCCTGC AGCCCGTACT CGCCCAACTC CCGGCCACTC
            181 CCCGACATCT TGTAGCCACC AAAGGGTGAC TGGGCTCCAA ACACATCATA GCAGTTGACC
            241 CACACAGTGC CCGCCTGGAG GGCCTGGGAC AGGTAATTGG CCTTGTCCAA ATCCTTTGTG
            301 AAGACAGCTG CGGCCAGCCC GTACGTGGAA TTGTTGGCTC TCCCAACAAC CTCCTCTATG
            361 GTCTTGAACT TCAGGATCTG CATCACTGGC CCGAAGATCT CCTCCTTGGC GATGGTCATG
            421 CCATCCTGCA CATCTCCAAA CACAGTGGGC TGGATGAAGT AACCACGGTC AGCAGCAATG
            481 CCCCCACCAC ACAGCAGCTT CGCCCCCTCT TGCTTCCCCG TGTTGATGTA GCCGAGGATC
            541 TTCTTAAACT GAGTTTCATC CACCTGCGGC CCCTGCTCGG TCTTGCTATC AAAGGGGTTC
            601 CCGACCACCC GAGACTTGGC CCGGGCAACG CTCCGCTCCA CAAACTCATC ATAGATGTCC
            661 TCCTGCACGA AGGTCCGGGA GCCGGCACAG CAGCACTGGC CCTGGTTGAA GAACAGGGCG
            721 AAGTGGGCCT GTTCCACGGC CCAATCCATA TCGGCATCTG ACATGATGAT GTTGGGGCTC
            781 TTCCCCCCCA GCTCCAAGGG TCACTCTCTT GAGGTTGCTG CTCCCAGCAG CAACCTGGAT
            841 TACGCGGCCA ATCTCAGTGG GAGCCTGTGA ATGCCACTTT GTCCACATCC TCATGGGAGG
            901 CAATGGGCGG CCCCAGCCGT GGGGGCCAAA TCCAGGCACA AATGTTGACC ACACCAAGGG
            961 GGAAAAGCCA GCCTCCTTTG ATCCAGGTTG GGCCACATAA AGGGGCGGTG AGGGGGTTGT
           1021 CTTGGCTTCA GGCTAACCTT TCAATCACAA CCCACGGTTT TCCCAGGTTT GCCAAAGGGG
           1081 CTGGAGCTCC AAGCTTTCCA TTGCTTGCAT TCAAGGGAAC GGGAAAATTC CCCACGGGAA
           1141 ATGAATTCTG CCCCGTCAAA CCTCCTACAA GGTTCATGGG CACGGTGGTG TTATGACCAT
          1201 CGAAGAGAAA AATCCTCTCC GGTC
          //
          Primer: CMV_en_R
          Sequence file: RDB13742_A6A5c.seq check
          >D02971A3_A6A5_1_CMV_en_R_C10_03_ABI08.ab1
              1 GGGTCTAATT GAATTACTAT TAATAACTAA TGCATGGCGG TAATACGTCT AGAACCTCGG
             61 ACCTGCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
            121 CTCACTATAG GAGGGCCACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
            181 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
            241 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
            301 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
            361 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
            421 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
            481 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGATTCCG GACTAGAGCG GGCCCGGTAC
            541 TAACCAAACT GGATCTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT
            601 TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC
            661 TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG
            721 TCTGCAGAAT TGGCGCACGC AGTGCCGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT
            781 CTCTCTCTCC ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA
            841 GGTGGGTCTG AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA
            901 CAGCAAAAAC TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAA TAATTTTATA
            961 TACCACAGCC AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTTC
           1021 CAATAATTCC TGTTCATTTC TTTTCTTGCT GGATTTTGCC GAATCTCCAA TTAAACGAGT
          1081 GTATTAAGCT GTGTATGGTA TTTTCTCTGT TCCCCACTTC CATTCCAAGG CTTCG
          //
          Primer: IRES-F
          Sequence file: RDB13742_A6A5d.seq check
          >D02971A3_A6A5_1__IRES_F_D06_12.ab1
              1 ACACGGCAAG AAGAAGGAAG AGGAGAGGGG GGGGGGGGGG ATAAGATGTC GGAAGAAGGG
             61 TAAAAAAGAG GGGAGCCGAG CTTCGGGTTA TGAGTTAAAA CGTCTGGCCC CCCGAACCAC
            121 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG GCCACCGAGT ACAAGCCCAC
            181 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT
            241 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC
            301 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
            361 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
            421 GTTCGCCGAG ATCGGCTCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
            481 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
            541 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
            601 AGTGGAGGCG GCCGAGCGCG CTGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
            661 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
            721 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTTAACAAAT CAACCTCTGG
            781 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
            841 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
            901 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTTATG AAGGAGTTGT GGGCCCGTTG
            961 TCAGCAACGT GGGCGTGGTG TGCACTGTGT TTTGCTGACG CAACCCCCAC TGGTTGGGGG
           1021 CATTGCCACC ACCTGTCAGC TCCCTTTCCG GGGACTTTCG CTTTTCCCCC TTCCCCTATT
           1081 GGCCACGGGC GGGAAACATC AATCGCCGGC CCTTGCCCTT TGCCCGCTGG CCTTGGGAAC
           1141 AAGGGGGGGC CTCCGGCCTG GTTTGGGGGC CAACTTGAAC CAAAATTCCG TTGGGGTGTG
          1201 TTTTTGTTTC CC
          //
          Primer: HIVgp_R1
          Sequence file: RDB13742_A6A5e.seq check
          >D02971A3_A6A5_1__HIVgP_R1_E06_15.ab1
              1 GCGGCATCCT TATTTCCCAC TGGCTCTTTT TTCTCTCTGC ACCACCTCTT CTCTTTGCCC
             61 TTGGTGGGTG CTACTCCTAA CTGTTTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC
            121 TTCTCCAATT GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG
            181 CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT
            241 GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA
            301 TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA
            361 GCCTTCTGAT GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC
            421 CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC
            481 CCATCGCGAT CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT
            541 CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC
            601 CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC
            661 TTTCAAGTCC CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG
            721 AGGGATCTCT AGTTACCAGA GTCACACAAA CAGACGGGCA CACACTACTT GAAGCACTCA
            781 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC
            841 TCAGATCTGG TCTAACCAAG AGAGACCCAG TAACAGGCAA AAAAGCAGCT GCTTATATGC
            901 AGGAATCTGA GGGCTCGCCA CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC
            961 CCAGCGGAAA GTCCCTTGTA GCAAGCTCGA TAATCAGCAG TTCTTGGAGT ACTCCCGGAT
           1021 GCAGCTCTCG GGCCACGTGA ATGAAATGCT AAGCGGCTGT CAAACCCTCC ACTCTAAACA
           1081 CTTCTCTCTC CCGGTCATCC ATCCATGCCA GCCTCACAAG GTGGTAACAG CCTGTGTTCT
          1141 CCTTCCTTAA TGGCCCTCTT CTTACCTATA ACTCTGGGCC CTCCACACTG TGGGTT
          //
          Primer: BGH_rev
          Sequence file: RDB13742_A6A5f.seq check
          >D02971A3_A6A5_1_BGH_rev_F06_18.ab1
              1 CATGATTCAA GGTCAAGGGC CCCTCTTCAC TCTCTGAATA TTCATTTCTT TGCAAGTTAT
             61 AAATACTGAA TAATAAGATG ACATGAACTA CTACTGCTAG AGATTTTCCA CACTGACTAA
            121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
            181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
            241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AAGCAAAAAG CAGATCTTGT
            301 CTTCGTTGGG AGTGAATTAG CCCTTCCAGT CCCCTCTTTT CTTTTAAAAA GTGGCTAAGA
            361 TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT GAGGTGTGAC TGGAAAACCC
            421 ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG CATTGGTAGC TGCTGTATTG
            481 CTACTTGTGA TTGCTCCATG TTTTTCTAGG TCTCGACTGC AGAATTAATT CCAGGCGGGG
            541 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
            601 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
            661 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
            721 ACGTCAGCTT CCCCGACAAA CACCACGGAA TTGTCAGTGC CCCAACAGCC GAGCCCCTGT
            781 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCGCCGTG GCAATAGGGA GGGGGAAAGC
            841 GAAAGTCCCG GAAAGGAGCT GACAGGTGGT GGCAATGCCC CAACCAGTGG GGGTTGCGTC
            901 AGCAAAACAC AGTGCACACC ACGCCACGTT GCCTGACAAC GGGGCCCACA ACTCCTCATA
            961 AAGGAGACAG CAACCAGGAT TTATACAGGG AGGAAGAAAA ATGAAAGCCC ATACGGGAAA
           1021 GCAAATAGCA TGATACAAAA GGGCATTAAA GCAGCGGTAA TCCACATAGC GTAAAAGGGA
           1081 GCAAACATAG TTAGAATAAC CAGGTCAATC TTTTCACCAA ATTTTTGTTA TCCAGAAGGG
           1141 TTGGATTGGT AAACTCAGGC ACGGCCATGG CGGATCAATG CACCAAGGGT GCGCGGTTCC
          1201 CTTCGGCGGG CACCTCTT
          //
          Primer: SV40pro_F
          Sequence file: RDB13742_A6A5g.seq check
          >D02971A3_A6A5_1_SV40pro_F_D10_04_ABI08.ab1
              1 CCCCTGTGAA CAATTCCAAG GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
             61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GATGAAAAAG
            121 CCTGAACTCA CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC
            181 GACCTGATGC AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG
            241 CGTGGATATG TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT
            301 TATCGGCACT TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC
            361 AGCGAGAGCC TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG
            421 CCTGAAACCG AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT
            481 GCGGCCGATC TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA
            541 TACACTACAT GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA
            601 ACTGTGATGG ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT
            661 TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT
            721 GTCCTGACGG ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG
            781 GATTCCCAAT ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG
            841 CAGCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG
            901 GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC
            961 GATGATGCAG CTTGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCCGGGACT
           1021 GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA
           1081 GGTACTCGCC GAATAGTGAA TCGGAACGGA CCCAAGCACT CGTTCGAAAG GGCCAAAGGG
          1141 AAAATAAGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Amanuma, Y., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl