Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLEX_ALDH2Glu504Lys (#RDB13742)

Lentiviral plasmid vector of FLAG-tagged mutant ALDH2

Clone info. Lentiviral plasmid vector of FLAG-tagged mutant ALDH2 (Glu504Lys). This point mutation causes catalytic deficiency of human ALDH2.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pLEX-MCS (plasmid)
Size of vector backbone 10.7 kb
Selectable markers Ampicillin, Zeocin (E. coli), Puromycin (mammalian cells).
Gene/insert name human ALDH2 cDNA
Depositor|Developer Muto, Manabu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13742 pLEX_ALDH2Glu504Lys Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLEX_ALDH2Glu504Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13742).

Reference section:

Amanuma, Y., Ohashi, S., Itatani, Y., Tsurumaki, M., Matsuda, S., Kikuchi, O., Nakai, Y., Miyamoto, S., Oyama, T., Kawamoto, T., Whelan, K.A., Nakagawa, H., Chiba, T., Matsuda, T., Muto, M.., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13742_A6A5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB13742_A6A5a.seq check
>D02971A3_A6A5_1_CMV_Forward_B02_05.ab1
    1 CGGGTTAACT CCGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC GCTGTTTTGA
  121 CCTCCATAGA AGACACCGAC TCTACTAGAG GATCCACTAG CGATTACGCC AAGCTCGAAA
  181 TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTCCACCG CGGTGGCGGC CGCCACCATG
  241 GATTACAAGG ATGACGACGA TAAGAGCCCG GGCGGATCCC CCGGGCTGCA GGAATTCATG
  301 TTGCGCGCTG CCGCCCGCTT CGGGCCCCGC CTGGGCCGCC GCCTCTTGTC AGCCGCCGCC
  361 ACCCAGGCCG TGCCTGCCCC CAACCAGCAG CCCGAGGTCT TCTGCAACCA GATTTTCATA
  421 AACAATGAAT GGCACGATGC CGTCAGCAGG AAAACATTCC CCACCGTCAA TCCGTCCACT
  481 GGAGAGGTCA TCTGTCAGGT AGCTGAAGGG GACAAGGAAG ATGTGGACAA GGCAGTGAAG
  541 GCCGCCCGGG CCGCCTTCCA GCTGGGCTCA CCTTGGCGCC GCATGGACGC ATCACACAGG
  601 GGCCGGCTGC TGAACCGCCT GGCCGATCTG ATCGAGCGGG ACCGGACCTA CCTGGCGGCC
  661 TTGGAGACCC TGGACAATGG CAAGCCCTAT GTCATCTCCT ACCTGGTGGA TTTGGACATG
  721 GTCCTCAAAT GTCTCCGGTA TTATGCCGGC TGGGCTGATA AGTACCACGG GAAAACCATC
  781 CCCATTGACG GAGACTTCTT CAGCTACACA CGCCATGAAC CTGTGGGGGT GTGCGGGCAG
  841 ATCATTCCGT GGAATTTCCC GCTCCTGATG CAAGCATGGA AGCTGGGCCC AGCCTTGGCA
  901 ACTGGAAACG TGGTTGTGAT GAAGGTAGCT GAGCAGACAC CCCCTCACCG CCCTCTATGT
  961 GGCCCAACCC TGATCAAGGA GGCTGGCTTT TCCCCCCTGG TGTGGTCAAC ATTTGTGCCC
 1021 TGCATTTGGC CCCCCACGGC TGGGGGCGGC CCATTGCCTC CCATGGAGGA ATGTGATAAA
1081 GTGGCAATTC CACCAAGGAC TTCCAACA
//
Primer: IRES-R ()
Sequence file: RDB13742_A6A5b.seq check
>D02971A3_A6A5_1__IRES_R_C06_09.ab1
    1 GAATATCGGG GCTTTGGGCC AGTACGTTTA GGGGGGGGGG AGGGGAGAGG GGGCGACCGG
   61 TGGCCAGACG CGTCGGGCCC TCTAGACTCG AGTTATGAGT TCTTCTGAGG CACTTTGACT
  121 GTGACAGTTT TCACTTTAGT GTATGCCTGC AGCCCGTACT CGCCCAACTC CCGGCCACTC
  181 CCCGACATCT TGTAGCCACC AAAGGGTGAC TGGGCTCCAA ACACATCATA GCAGTTGACC
  241 CACACAGTGC CCGCCTGGAG GGCCTGGGAC AGGTAATTGG CCTTGTCCAA ATCCTTTGTG
  301 AAGACAGCTG CGGCCAGCCC GTACGTGGAA TTGTTGGCTC TCCCAACAAC CTCCTCTATG
  361 GTCTTGAACT TCAGGATCTG CATCACTGGC CCGAAGATCT CCTCCTTGGC GATGGTCATG
  421 CCATCCTGCA CATCTCCAAA CACAGTGGGC TGGATGAAGT AACCACGGTC AGCAGCAATG
  481 CCCCCACCAC ACAGCAGCTT CGCCCCCTCT TGCTTCCCCG TGTTGATGTA GCCGAGGATC
  541 TTCTTAAACT GAGTTTCATC CACCTGCGGC CCCTGCTCGG TCTTGCTATC AAAGGGGTTC
  601 CCGACCACCC GAGACTTGGC CCGGGCAACG CTCCGCTCCA CAAACTCATC ATAGATGTCC
  661 TCCTGCACGA AGGTCCGGGA GCCGGCACAG CAGCACTGGC CCTGGTTGAA GAACAGGGCG
  721 AAGTGGGCCT GTTCCACGGC CCAATCCATA TCGGCATCTG ACATGATGAT GTTGGGGCTC
  781 TTCCCCCCCA GCTCCAAGGG TCACTCTCTT GAGGTTGCTG CTCCCAGCAG CAACCTGGAT
  841 TACGCGGCCA ATCTCAGTGG GAGCCTGTGA ATGCCACTTT GTCCACATCC TCATGGGAGG
  901 CAATGGGCGG CCCCAGCCGT GGGGGCCAAA TCCAGGCACA AATGTTGACC ACACCAAGGG
  961 GGAAAAGCCA GCCTCCTTTG ATCCAGGTTG GGCCACATAA AGGGGCGGTG AGGGGGTTGT
 1021 CTTGGCTTCA GGCTAACCTT TCAATCACAA CCCACGGTTT TCCCAGGTTT GCCAAAGGGG
 1081 CTGGAGCTCC AAGCTTTCCA TTGCTTGCAT TCAAGGGAAC GGGAAAATTC CCCACGGGAA
 1141 ATGAATTCTG CCCCGTCAAA CCTCCTACAA GGTTCATGGG CACGGTGGTG TTATGACCAT
1201 CGAAGAGAAA AATCCTCTCC GGTC
//
Primer: CMV_en_R ()
Sequence file: RDB13742_A6A5c.seq check
>D02971A3_A6A5_1_CMV_en_R_C10_03_ABI08.ab1
    1 GGGTCTAATT GAATTACTAT TAATAACTAA TGCATGGCGG TAATACGTCT AGAACCTCGG
   61 ACCTGCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA
  121 CTCACTATAG GAGGGCCACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG CTCACCGCGC
  181 GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC CGGGACTTCG
  241 TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC AGCGCGGTCC
  301 AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC CTGGACGAGC
  361 TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC GGGCCGGCCA
  421 TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC CCGGCCGGCA
  481 ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGATTCCG GACTAGAGCG GGCCCGGTAC
  541 TAACCAAACT GGATCTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT
  601 TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC
  661 TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG
  721 TCTGCAGAAT TGGCGCACGC AGTGCCGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT
  781 CTCTCTCTCC ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA
  841 GGTGGGTCTG AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA
  901 CAGCAAAAAC TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAA TAATTTTATA
  961 TACCACAGCC AATTTGTTAT GTTAAACCAA TTCCACAAAC TTGCCCATTT ATCTAATTTC
 1021 CAATAATTCC TGTTCATTTC TTTTCTTGCT GGATTTTGCC GAATCTCCAA TTAAACGAGT
1081 GTATTAAGCT GTGTATGGTA TTTTCTCTGT TCCCCACTTC CATTCCAAGG CTTCG
//
Primer: IRES-F ()
Sequence file: RDB13742_A6A5d.seq check
>D02971A3_A6A5_1__IRES_F_D06_12.ab1
    1 ACACGGCAAG AAGAAGGAAG AGGAGAGGGG GGGGGGGGGG ATAAGATGTC GGAAGAAGGG
   61 TAAAAAAGAG GGGAGCCGAG CTTCGGGTTA TGAGTTAAAA CGTCTGGCCC CCCGAACCAC
  121 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG GCCACCGAGT ACAAGCCCAC
  181 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT
  241 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC
  301 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
  361 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
  421 GTTCGCCGAG ATCGGCTCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
  481 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
  541 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
  601 AGTGGAGGCG GCCGAGCGCG CTGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
  661 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
  721 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTTAACAAAT CAACCTCTGG
  781 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT
  841 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
  901 TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTTATG AAGGAGTTGT GGGCCCGTTG
  961 TCAGCAACGT GGGCGTGGTG TGCACTGTGT TTTGCTGACG CAACCCCCAC TGGTTGGGGG
 1021 CATTGCCACC ACCTGTCAGC TCCCTTTCCG GGGACTTTCG CTTTTCCCCC TTCCCCTATT
 1081 GGCCACGGGC GGGAAACATC AATCGCCGGC CCTTGCCCTT TGCCCGCTGG CCTTGGGAAC
 1141 AAGGGGGGGC CTCCGGCCTG GTTTGGGGGC CAACTTGAAC CAAAATTCCG TTGGGGTGTG
1201 TTTTTGTTTC CC
//
Primer: HIVgp_R1 ()
Sequence file: RDB13742_A6A5e.seq check
>D02971A3_A6A5_1__HIVgP_R1_E06_15.ab1
    1 GCGGCATCCT TATTTCCCAC TGGCTCTTTT TTCTCTCTGC ACCACCTCTT CTCTTTGCCC
   61 TTGGTGGGTG CTACTCCTAA CTGTTTTCAA TTTTTACTAC TTTATATTTA TATAATTCAC
  121 TTCTCCAATT GTCCCTCATA TCTCCTCCTC CAGGTCTGAA GATCAGCGGC CGGCCGCTTG
  181 CTGTGCGGTG GTCTTACTTT TGTTTTGCTC TTCCTCTATC TTGTCTAAAG CTTCCTTGGT
  241 GTCTTTTATC TCTATCCTTT GATGCACACA ATAGAGGGTT GCTACTGTAT TATATAATGA
  301 TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA
  361 GCCTTCTGAT GTTTCTAACA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC
  421 CCATACTATA TGTTTTAATT TATATTTTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC
  481 CCATCGCGAT CTAATTCTCC CCCGCTTAAT ACTGACGCTC TCGCACCCAT CTCTCTCCTT
  541 CTAGCCTCCG CTAGTCAAAA TTTTTGGCGT ACTCACCAGT CGCCGCCCCT CGCCTCTTGC
  601 CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC
  661 TTTCAAGTCC CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG
  721 AGGGATCTCT AGTTACCAGA GTCACACAAA CAGACGGGCA CACACTACTT GAAGCACTCA
  781 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC
  841 TCAGATCTGG TCTAACCAAG AGAGACCCAG TAACAGGCAA AAAAGCAGCT GCTTATATGC
  901 AGGAATCTGA GGGCTCGCCA CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC
  961 CCAGCGGAAA GTCCCTTGTA GCAAGCTCGA TAATCAGCAG TTCTTGGAGT ACTCCCGGAT
 1021 GCAGCTCTCG GGCCACGTGA ATGAAATGCT AAGCGGCTGT CAAACCCTCC ACTCTAAACA
 1081 CTTCTCTCTC CCGGTCATCC ATCCATGCCA GCCTCACAAG GTGGTAACAG CCTGTGTTCT
1141 CCTTCCTTAA TGGCCCTCTT CTTACCTATA ACTCTGGGCC CTCCACACTG TGGGTT
//
Primer: BGH_rev ()
Sequence file: RDB13742_A6A5f.seq check
>D02971A3_A6A5_1_BGH_rev_F06_18.ab1
    1 CATGATTCAA GGTCAAGGGC CCCTCTTCAC TCTCTGAATA TTCATTTCTT TGCAAGTTAT
   61 AAATACTGAA TAATAAGATG ACATGAACTA CTACTGCTAG AGATTTTCCA CACTGACTAA
  121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
  181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
  241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AAGCAAAAAG CAGATCTTGT
  301 CTTCGTTGGG AGTGAATTAG CCCTTCCAGT CCCCTCTTTT CTTTTAAAAA GTGGCTAAGA
  361 TCTACAGCTG CCTTGTAAGT CATTGGTCTT AAAGGTACCT GAGGTGTGAC TGGAAAACCC
  421 ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG GCACAATCAG CATTGGTAGC TGCTGTATTG
  481 CTACTTGTGA TTGCTCCATG TTTTTCTAGG TCTCGACTGC AGAATTAATT CCAGGCGGGG
  541 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC
  601 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT
  661 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG
  721 ACGTCAGCTT CCCCGACAAA CACCACGGAA TTGTCAGTGC CCCAACAGCC GAGCCCCTGT
  781 CCAGCAGCGG GCAAGGCAGG CGGCGATGAG TTCCGCCGTG GCAATAGGGA GGGGGAAAGC
  841 GAAAGTCCCG GAAAGGAGCT GACAGGTGGT GGCAATGCCC CAACCAGTGG GGGTTGCGTC
  901 AGCAAAACAC AGTGCACACC ACGCCACGTT GCCTGACAAC GGGGCCCACA ACTCCTCATA
  961 AAGGAGACAG CAACCAGGAT TTATACAGGG AGGAAGAAAA ATGAAAGCCC ATACGGGAAA
 1021 GCAAATAGCA TGATACAAAA GGGCATTAAA GCAGCGGTAA TCCACATAGC GTAAAAGGGA
 1081 GCAAACATAG TTAGAATAAC CAGGTCAATC TTTTCACCAA ATTTTTGTTA TCCAGAAGGG
 1141 TTGGATTGGT AAACTCAGGC ACGGCCATGG CGGATCAATG CACCAAGGGT GCGCGGTTCC
1201 CTTCGGCGGG CACCTCTT
//
Primer: SV40pro_F ()
Sequence file: RDB13742_A6A5g.seq check
>D02971A3_A6A5_1_SV40pro_F_D10_04_ABI08.ab1
    1 CCCCTGTGAA CAATTCCAAG GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GATGAAAAAG
  121 CCTGAACTCA CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC
  181 GACCTGATGC AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG
  241 CGTGGATATG TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT
  301 TATCGGCACT TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC
  361 AGCGAGAGCC TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG
  421 CCTGAAACCG AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT
  481 GCGGCCGATC TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA
  541 TACACTACAT GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA
  601 ACTGTGATGG ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT
  661 TGGGCCGAGG ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT
  721 GTCCTGACGG ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG
  781 GATTCCCAAT ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG
  841 CAGCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG
  901 GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC
  961 GATGATGCAG CTTGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCCGGGACT
 1021 GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA
 1081 GGTACTCGCC GAATAGTGAA TCGGAACGGA CCCAAGCACT CGTTCGAAAG GGCCAAAGGG
1141 AAAATAAGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Amanuma, Y., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]