Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLEX_ALDH2WT (#RDB13741)

Lentiviral plasmid vector of FLAG-tagged ALDH2

Clone info. Lentiviral plasmid vector of FLAG-tagged ALDH2 for establish a stably transfected cell line expressing human ALDH2.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pLEX-MCS (plasmid)
Size of vector backbone 10.7 kb
Selectable markers Ampicillin, Zeocin (E. coli), Puromycin (mammalian cells).
Gene/insert name human ALDH2 cDNA
Depositor|Developer Muto, Manabu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13741 pLEX_ALDH2WT Please contact us. JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLEX_ALDH2WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13741).

Reference section:

Amanuma, Y., Ohashi, S., Itatani, Y., Tsurumaki, M., Matsuda, S., Kikuchi, O., Nakai, Y., Miyamoto, S., Oyama, T., Kawamoto, T., Whelan, K.A., Nakagawa, H., Chiba, T., Matsuda, T., Muto, M.., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13741_A6A5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward ()
Sequence file: RDB13741_A6A5a.seq check
>D02971A2_A6A5_1__CMV_Forward_E05_14.ab1
    1 CTTAATCACC TCCGGCCCCA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA TCCACGCTGT
  121 TTTGACCTCC ATAGAAGACA CCGACTCTAC TAGAGGATCC ACTAGCGATT ACGCCAAGCT
  181 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCCA
  241 CCATGGATTA CAAGGATGAC GACGATAAGA GCCCGGGCGG ATCCCCCGGG CTGCAGGAAT
  301 TCATGTTGCG CGCTGCCGCC CGCTTCGGGC CCCGCCTGGG CCGCCGCCTC TTGTCAGCCG
  361 CCGCCACCCA GGCCGTGCCT GCCCCCAACC AGCAGCCCGA GGTCTTCTGC AACCAGATTT
  421 TCATAAACAA TGAATGGCAC GATGCCGTCA GCAGGAAAAC ATTCCCCACC GTCAATCCGT
  481 CCACTGGAGA GGTCATCTGT CAGGTAGCTG AAGGGGACAA GGAAGATGTG GACAAGGCAG
  541 TGAAGGCCGC CCGGGCCGCC TTCCAGCTGG GCTCACCTTG GCGCCGCATG GACGCATCAC
  601 ACAGGGGCCG GCTGCTGAAC CGCCTGGCCG ATCTGATCGA GCGGGACCGG ACCTACCTGG
  661 CGGCCTTGGA GACCCTGGAC AATGGCAAGC CCTATGTCAT CTCCTACCTG GTGGATTTGG
  721 ACATGGTCCT CAAAATGTCT CCGGTATTAT GCCGGCTGGG CTGATAAAGT ACCACGGGGA
  781 AAACCATCCC CATTGACGGA GACTTCTTCA GCTACACACG CCATGAACCC TGTGGGGGTG
  841 TGCGGGCAGA TCATTCCGTG GAATTTCCCG CTCCTGATGC AAGCATGGGA AGCTGGGGCC
  901 CAGCCCTTGG CAACTGGAAA CGTGGGTTGT GATGAAAGGT AGCTGGAGCA GACACCCCTT
  961 CACGGCCCTC TTATGTTGGC CAACCTGGAT CAAGGGAGCT GGGCTTTCCC CCCTTGTGGT
 1021 TGGTTCAAAC ATTGGTGGCC TGGGATTGGG CCCACGGCTT GGGGCCGCAA TTGCCTTCCA
 1081 TGGGAGCATG TGGACAAAGG TGCAATCCAG CGTTCACTGA ATTGGCCCGG TATCAGTTGA
1141 CTGTCGCTGG GGAGCA
//
Primer: IRES-R ()
Sequence file: RDB13741_A6A5b.seq check
>D02971A2_A6A5_1__IRES_R_F05_17.ab1
    1 CTTCCGCCGG GGCCTTCCGG GCAGGTAACG GTTAGGGGGG AGGGGAGGGG AGAGGGGGCG
   61 ACCGGTGGCC AGACGCGTCG GGCCCTCTAG ACTCGAGTTA TGAGTTCTTC TGAGGCACTT
  121 TGACTGTGAC AGTTTTCACT TCAGTGTATG CCTGCAGCCC GTACTCGCCC AACTCCCGGC
  181 CACTCCCCGA CATCTTGTAG CCACCAAAGG GTGACTGGGC TCCAAACACA TCATAGCAGT
  241 TGACCCACAC AGTGCCCGCC TGGAGGGCCT GGGACAGGTA ATTGGCCTTG TCCAAATCCT
  301 TTGTGAAGAC AGCTGCGGCC AGCCCGTACG TGGAATTGTT GGCTCTCCCA ACAACCTCCT
  361 CTATGGTCTT GAACTTCAGG ATCTGCATCA CTGGCCCGAA GATCTCCTCC TTGGCGATGG
  421 TCATGCCATC CTGCACATCT CCAAACACAG TGGGCTGGAT GAAGTAACCA CGGTCAGCAG
  481 CAATGCCCCC ACCACACAGC AGCTTCGCCC CCTCTTGCTT CCCCGTGTTG ATGTAGCCGA
  541 GGATCTTCTT AAACTGAGTT TCATCCACCT GCGGCCCCTG CTCGGTCTTG CTATCAAAGG
  601 GGTTCCCGAC CACCCGAGAC TTGGCCCGGG CAACGCTCCG CTCCACAAAC TCATCATAGA
  661 TGTCCTCCTG CACGAAGGTC CGGGAGCCGG CACAGCAGCA CTGGCCCTGG TTGAAAGAAC
  721 AGGGCGAAGT GGGCCTGTTC CACGGCCCAA TCCATATCGG CATCTGACAT GATGATGTTG
  781 GGGCTCTTCC CCCCCCAGCT CCAAGGTCAC TCTCTTGAGG TTGCTGCTCC CAGCAGCAAC
  841 CTGGATTACG CGGCCAATCT CAGTGGAGCC TGTGAATGCC ACTTTTGTCC CACATCCTTC
  901 ATGGGGAAGG CAATGGCGGG CCCCAGCACG TGGGGCCAAA ATCCCAGGCC ACAATGTTGA
  961 CCACACCCAG GGGGGAAAGC CAGCCCTCCT TGATCAGGGT TGGGCCACAT AGAAGGGCGG
 1021 GTGAGGGGTG GTCTGCCTCA GCTAACCTTT CATTCACAAA CCACCGTTTT CCAAGTTTGC
 1081 CCAAGGCTGG AGCCCAAGCT TTCCATGGCC TTGCCATCGA GAAGCGGGAA AATTTCCAAC
1141 GGGAAATGGA ATGCTTGCCC CGGCAACATC CCCCCCTACT ACAAGTGTTC TCATTGGT
//
Primer: CMV_en_R ()
Sequence file: RDB13741_A6A5c.seq check
>D02971A2_A6A5_1_CMV_en_R_A10_01_ABI08.ab1
    1 TCCGGTATAT GATTACTATT AATAACTAAT GCATGGCGGT AATACGTCTA GAACCTCGGA
   61 CCTGCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC
  121 TCACTATAGG AGGGCCACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG
  181 CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT
  241 GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA
  301 GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT
  361 GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT
  421 GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA
  481 CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGATTCCGG ACTAGAGCGG GCCCGGTACT
  541 AACCAAACTG GATCTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT TTTTGTAATT
  601 TGTTTTTGTA ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT TCTTTCCCCT
  661 GCACTGTACC CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC TGCCATTTGT
  721 CTGCAGAATT GGCGCACGCA GTGCCGATCC GTTCACTAAT CGAATGGATC TGTCTCTGTC
  781 TCTCTCTCCA CCTTCTTCTT CTATTCCTTC GGGCCTGTCG GGTCCCCTCG GGGTTGGGAG
  841 GTGGGTCTGA AACGATAATG GTGAATATCC CTGCCTAACT CTATTCACTA TAGAAAGTAC
  901 AGCAAAAACT ATTCTTAAAC CTACCAAGCC TCCTACTATC ATTATGAATA ATTTTATATA
  961 CCACAGCCAA TTTGTTATGT TAAAACCAAT TCCACAAACT TGCCCATTTA TCTAAATTCC
 1021 CAATAAATTT CTTGTTCATT CTTTTCTTGC TGGGTTTTGC GAATTCTTTC CATTTAAAGA
1081 GTGTATAAGG CCTGCTGTAT GGTAATTTCT CTGATCCCCA CCTCCAATTC CCCAGGGA
//
Primer: IRES-F ()
Sequence file: RDB13741_A6A5d.seq check
>D02971A2_A6A5_1__IRES_F_G05_20.ab1
    1 CAAATCCTTT AAATGTTGTT TAGTCGAGGT TAAAAAAAAC GTCTAGGCCC CCCGAACCAC
   61 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG GCCACCGAGT ACAAGCCCAC
  121 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT
  181 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC
  241 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
  301 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
  361 GTTCGCCGAG ATCGGCTCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
  421 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
  481 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
  541 AGTGGAGGCG GCCGAGCGCG CTGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
  601 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
  661 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTTAACAATC AACCTCTGGA
  721 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGGCTAT
  781 GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT
  841 TCTCCTCCTT GTATAAATCC TGGGTTGCTG TCTCTTTATG AGGAGTTTGT GGGCCCGTTT
  901 GTCAGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCCAC TGGTTGGGGG
  961 CATTTGCCAC CAACCTGTCA GCTCCTTTCC CGGGACTTTC GCTTTCCCCC CTCCCTATTG
 1021 CCACGGCGGA AACTCATCGC CGCTTGCCTT GCCGCTGCTG GACAAGGGGC CTCGGCCTGT
 1081 TGGGCCACTG ACAATTTCCG TGGGTGTTGG TCGGGGGAAA GCCTGAACGT TCCTTTACAA
1141 TGGCTGGCTT CGCCTGTTGA TTGTCCACCG TGAAATTTCT GTGCGCCG
//
Primer: HIVgp_R1 ()
Sequence file: RDB13741_A6A5e.seq check
>D02971A2_A6A5_1__HIVgP_R1_H05_23.ab1
    1 TCGGCTTCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
   61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
  121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG
  181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA
  241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT
  301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG
  361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA
  421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG
  481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC
  541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG
  601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT
  661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGAGGGATCT
  721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT
  781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG AGCTCCCAGG CTCAGATCTG
  841 GTCTAACCAG AGAGACCCAG TACAGGCAAA AAGCAGCTGC TTATATGCAG GATCTGAGGG
  901 CTCGCCACTC CCCAGTCCCG CCCAGGCCAC GCCTCCCTGG GAAAGTCCCC AGCGGAAAGT
  961 CCCTTGTAGC AAGCTCGATA TCAGCAGTTC TTGAAGTACT CCGGGATGCA GCTCTCGGGC
 1021 CACGTGATGA AATGCTAGGC GGGCTGTCAA ACCTCACTCT AACACTTCTT CTTCTCCGGG
 1081 GTCATCATCC CATGCAGCTC CACAGGTGGT TAACAAGGCT GGTGTCTCCT CCATTTATTG
1141 GCTCTTCTAA CTTATCTTGG CTCACTGTAC ATAGCCTGTA AGCCACTCTA ACT
//
Primer: BGH_rev ()
Sequence file: RDB13741_A6A5f.seq check
>D02971A2_A6A5_1_BGH_rev_A06_03.ab1
    1 AGGTTTTAAA GGGTCAAGGC CTTCTCACTC TCTGATATTC ATTTCTTTGC AAGTTATAAA
   61 TACTGAATAA TAAGATGACA TGAACTACTA CTGCTAGAGA TTTTCCACAC TGACTAAAAG
  121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC
  181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC
  241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAAG CAAAAAGCAG ATCTTGTCTT
  301 CGTTGGGAGT GAATTAGCCC TTCCAGTCCC CTCTTTTCTT TTAAAAAGTG GCTAAGATCT
  361 ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA GGTACCTGAG GTGTGACTGG AAAACCCACC
  421 TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA CAATCAGCAT TGGTAGCTGC TGTATTGCTA
  481 CTTGTGATTG CTCCATGTTT TTCTAGGTCT CGACTGCAGA ATTAATTCCA GGCGGGGAGG
  541 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA
  601 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
  661 AGAAGGACGT CCCGCGCAGA ATCCAGGGTG GCAAACACAG GCGAGCAGCC ATGGAAAGGA
  721 CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC AAACAGCCGA GCCCCTGTCC
  781 AGCAGCGGGC AAGGCAGGCG GCGATGAGTT CCGCCGTGGC AATAGGGAGG GGGGAAAGCG
  841 AAAGTCCCGG AAAAGGGAGC TGACAGGTGG TGGCAATGCC CCAACCAGTG GGGGTTGCGG
  901 TCAGCAAAAC ACAGTGCACA CCACGCCACG TTGCCCTGAC AAACGGGGCC ACAACTCCTC
  961 ATTAAAGGAG GACAGCAACC AAGGATTTAT ACAAGGGAAG GAGGAAAAAT GAAAGCCAAT
 1021 ACGGGGAAGC AATAGCAATG GATACAAAGG GCATTTAAAA GCAGCCGTTA TTCCCACATA
 1081 GCGTAAAAGG GAGGCAAACA TTAGTTAGAG GAAATACCAA GTTCAATCTT TTTCAACAAA
 1141 ATTTTTGGTT AATTCCCAGG AAGGGTTTGA TTGTTACTTC AGGCACCCGG GGCTTGCGAG
1201 GGTTCACATG GCACCTAGGG GTGTCGCCGC AGGT
//
Primer: SV40pro_F ()
Sequence file: RDB13741_A6A5g.seq check
>D02971A2_A6A5_1_SV40pro_F_B10_02_ABI08.ab1
    1 CCTTGTAACA TTCAGGAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG
   61 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGATGAA AAAGCCTGAA
  121 CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG
  181 ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA
  241 TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG
  301 CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG
  361 AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA
  421 ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC
  481 GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT
  541 ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG
  601 ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC
  661 GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG
  721 ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC
  781 CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG
  841 ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT
  901 ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG GTTGACGGCA ATTTCGATGA
  961 TGCAGCTTGG CGCAGGGTCG ATGCGACGCA AATCGTCCGA TCCGGAGCCG GGACTGTCGG
 1021 GCGTACACAA TCGGCCCGCA GAGCGCGGCC GTCTGGGACC GGAATGACTG TGTAGAAGTA
 1081 CTCGCCGATA GTGAACGGAA CGCCCTAGCA CTCGGTCCGG AGGGGCCAAA AAGGCAAATA
1141 AGTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Amanuma, Y., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]