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pCMV-Tag2B_ALDH2 (#RDB13740)

Expression vector of FLAG-tagged human ALDH2.

Clone info. Expression vector of FLAG-tagged human ALDH2.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pCMV-Tag2B (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cels).
Gene/insert name human ALDH2 cDNA
Depositor|Developer Muto, Manabu |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Amanuma, Y., Sci. Rep. 5: 14142, 2015)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Amanuma, Y., Sci. Rep. 5: 14142, 2015)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13740 pCMV-Tag2B_ALDH2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-Tag2B_ALDH2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13740).

Reference section:

Amanuma, Y., Ohashi, S., Itatani, Y., Tsurumaki, M., Matsuda, S., Kikuchi, O., Nakai, Y., Miyamoto, S., Oyama, T., Kawamoto, T., Whelan, K.A., Nakagawa, H., Chiba, T., Matsuda, T., Muto, M.., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13740_A6A5p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMVpro,T3pro,FLAG,insert5
Sequence file: RDB13740_A6A5a.seq check
>D02971A1_A6A5_1_CMV_Forward_B05_05.ab1
    1 TTGTTCAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GATTACGCCA AGCTCGAAAT
  121 TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTCCACCGC GGTGGCGGCC GCCACCATGG
  181 ATTACAAGGA TGACGACGAT AAGAGCCCGG GCGGATCCCC CGGGCTGCAG GAATTCATGT
  241 TGCGCGCTGC CGCCCGCTTC GGGCCCCGCC TGGGCCGCCG CCTCTTGTCA GCCGCCGCCA
  301 CCCAGGCCGT GCCTGCCCCC AACCAGCAGC CCGAGGTCTT CTGCAACCAG ATTTTCATAA
  361 ACAATGAATG GCACGATGCC GTCAGCAGGA AAACATTCCC CACCGTCAAT CCGTCCACTG
  421 GAGAGGTCAT CTGTCAGGTA GCTGAAGGGG ACAAGGAAGA TGTGGACAAG GCAGTGAAGG
  481 CCGCCCGGGC CGCCTTCCAG CTGGGCTCAC CTTGGCGCCG CATGGACGCA TCACACAGGG
  541 GCCGGCTGCT GAACCGCCTG GCCGATCTGA TCGAGCGGGA CCGGACCTAC CTGGCGGCCT
  601 TGGAGACCCT GGACAATGGC AAGCCCTATG TCATCTCCTA CCTGGTGGAT TTGGACATGG
  661 TCCTCAAATG TCTCCGGTAT TATGCCGGCT GGGCTGATAA GTACCACGGG AAAACCATCC
  721 CCATTGACGG AGACTTCTTC AGCTACACAC GCCATGAACC TGTGGGGGTG TGCGGGCAGA
  781 TCATTCCGTG GAATTTCCCG CTCCTGATGC AAGCATGGAA GCTGGGCCCA GCCTTGGCAA
  841 CTGGAAACGT GGTTGTGATG AAGGTAGCTG AGCAGACACC CCTCACCGCC CTCTATGTGG
  901 CCAACCTGAT CAAAGGAGGC TGGCTTTCCC CCTGGTGTGG TCAACATTGT GCCTGGATTT
  961 GGCCCCACGG CTGGGGCCGC CATTGCCCTC CCATGAGATG TGGACAAAGT GGCATTCACA
 1021 GCTCCACTGA GATTGGGCCG CGTATCCAGG TGCTGCTGGG GAGCAGCACT CAGAGAGTGA
 1081 CTGCAGCTGG GGGGAGAGCC CACATCATCA TGTCAGATGC CGGAATATGT GAATTGGGGG
1141 CG
//
Primer: SV40polyA_R2 (Pr0623)
Region: SV40p(A),T7pro,insert3'
Sequence file: RDB13740_A6A5b.seq check
>D02971A1_A6A5_1-SV40polyA_R2_E10_05_ABI08.ab1
    1 CATTAAAAGG CTGGCAATAA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA
   61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT
  121 GGCTGATTAT GATCATGAAC AGACTGTGAG GACTGAGGGG CCTGAAATGA GCCTTGGGAC
  181 TGTGAATCTA AAATACACAA ACAATTAGAA TCAGTAGTTT AACACATTAT ACACTTAAAA
  241 ATTGGATCTC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGAGTGAATT
  301 GTAATACGAC TCACTATAGG GCGAATTGGG TACACTTACC TGGTACCTTA ATTAATTAAG
  361 GTACCGGGCC CCCCCTCGAG TTATGAGTTC TTCTGAGGCA CTTTGACTGT GACAGTTTTC
  421 ACTTCAGTGT ATGCCTGCAG CCCGTACTCG CCCAACTCCC GGCCACTCCC CGACATCTTG
  481 TAGCCACCAA AGGGTGACTG GGCTCCAAAC ACATCATAGC AGTTGACCCA CACAGTGCCC
  541 GCCTGGAGGG CCTGGGACAG GTAATTGGCC TTGTCCAAAT CCTTTGTGAA GACAGCTGCG
  601 GCCAGCCCGT ACGTGGAATT GTTGGCTCTC CCAACAACCT CCTCTATGGT CTTGAACTTC
  661 AGGATCTGCA TCACTGGCCC GAAGATCTCC TCCTTGGCGA TGGTCATGCC ATCCTGCACA
  721 TCTCCAAACA CAGTGGGCTG GATGAAGTAA CCACGGTCAG CAGCAATGCC CCCACCACAC
  781 AGCAGCTTCG CCCCCTCTTG CTTCCCCGTG TTGATGTAGC CGAGGATCTT CTTAAACTGA
  841 GTTTCATCCA CCTGCGGCCC CTGCTCGGTC TTGCTATCAA AGGGGTTCCC GACCACCCGA
  901 GACTTGGCCC GGGCAACGCT CCGCTCCACA AACTCATCAT AGATGTCCTC CTGCACGAAA
  961 GTCCGGGAGC CGGCACAGCA GCACTGGGCC CTGGTTGAAG AACAGGCGAA GTGGGCCTGT
 1021 TCCACGGGCC CAATCCATAT CGGCATCTGA CATGATGATG TGGGGCTCTC CCCCCCAGCT
 1081 CCAGTCACTC TTTGAGTTGC TGCTCCAGCA GCAACTTGGA TTACGGGCGG GCCAAATCTC
//
Primer: SV40pro_F_V2 (Pr0154)
Region: SV40pro_ori,NeoR_KanR
Sequence file: RDB13740_A6A5c.seq check
>D02971A1_A6A5_1_sv40poly_F_v2_D05_11.ab1
    1 ACGAGCCTTT TTTTTTGGAG GCCTAGGGCT TTTTGGCAAG ATCGATCAAG AGACAGGATG
   61 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
  121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
  181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
  241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
  301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
  361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
  421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
  481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
  541 TGATCTGGAC GAAGAACATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
  601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
  661 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGGCGG
  721 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAAACTTG GGCGGCGAAT
  781 GGGGCTTGAC CCGCTTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGGCAA
  841 TCGCCTTCCT ATCGCCTTTC TTGAACGAGT TCCTTCTGAA CGGGACTCTT GGGGGTTCGA
  901 AATGAACCGA CCAAACCGAC GCCAAACCTG GCCATCGCGA GATTTCGAAT TCCCCCTCCG
  961 CCCTTCTATA AAAGTTTGGG CTTTCGGAAT CTTTTTCCGG GACGCCCGGC TGGGATGATC
 1021 CTCCAACGCG GGATCTCCAG GCTGGAATTT CTCCGCCCAC CCCTAGGGGG TAGGCTAACT
1081 GAAACCACGG GAAGGAGAAA AATTACCGGT AAACGGAAAA CTCCGCGTCC TAATGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Amanuma, Y., Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium. Sci. Rep. 5: 14142 (2015). PMID 26374466. [link to RRC of NBRP]