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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

B95. delta A

Escherichia coli BL21(DE3)-based host strain with no specific assignment of the UAG codon.

Catalog number RDB13711
Resource name B95. delta A
Clone info. Escherichia coli BL-21(DE3)-based host strain with no specific assignment of the UAG codon.
Comment prfA gene has been deleted. Use primers prfAnoATG: AAGCCTTCTATCGTTGCCAAAC (22 bases) and prfATAARev: TTATTCCTGCTCGGACAACG (20 bases) for checking that the 1080 bp fragment of prfA gene is not amplified.
Growth remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Depositor|Developer Sakamoto, Kensaku |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The materials are only available based on the written approval by the depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mukai, T., Sci. Rep. 5: 9699 2015.) and an acknowledgment to the DEPOSITOR are requested. The materials are only available for the user belonging to a nonprofit organization.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
            Derived from BL21(DE3). It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は寄託者から事前に提供承諾書を取得すること。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mukai, T., Sci. Rep. 5: 9699 2015.) を引用し、謝辞の表明を必要とする。非営利機関の利用者に限定する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書 [Word]
            寄託者による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD), 受入れ確認書(書式J)
            BL21(DE3)を親株とする組換え体リソースです。実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
            組換え体提供にかかる書式が必要です。
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13711 B95. delta A Bacteria in stab agar

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Mukai, T., Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon. Sci. Rep. 5: 9699 (2015). PMID 25982672.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (English text)
            Featured content Dnaconda's recommendation EXR0072e (English text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)
            Featured content Incorporation of synthetic amino acids into proteins at specific sites [link] (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17937_B0Cgp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Mukai, T., Highly reproductive Escherichia coli cells with no specific assignment to the UAG codon. Sci. Rep. 5: 9699 (2015). PMID 25982672.
            reference Hauf, M., Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code. Chembiochem. 18 (18): 1819-1823 (2017). PMID 28650092.
            reference Mukai, T., Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli. Nucleic Acids Res. 43 (16): 8111-8122 (2015). PMID 26240376.
            protocol Seki, E., Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains. Methods Mol. Biol. 1728: 49-65 (2018). PMID 29404990.
            user_report Thyer, R., Directed Evolution of an Improved Aminoacyl-tRNA Synthetase for Incorporation of L-3,4-Dihydroxyphenylalanine (L-DOPA). Angew. Chem. Int. Ed. Engl. 60(27): 14811-14816 (2021). PMID 33871147.
            user_report Sapienza, P.J., Visualizing an Allosteric Intermediate Using CuAAC Stabilization of an NMR Mixed Labeled Dimer. ACS Chem. Biol. 16 (12): 2766-2775 (2021). PMID 34784173.

            2022.05.17

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