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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEMHE-mEGFP-INCENP

Plasmid for in vitro transcription of human INCENP in mouse oocyte.

Catalog number RDB13519
Resource name pGEMHE-mEGFP-INCENP
Clone info. Plasmid for in vitro transcription of human INCENP in mouse oocyte. Fused with mEGFP.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name human INCENP cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |
Other clones in our bank

External Database
human INCENP Aequorea victoria GFP
    Xenopus laevis beta-globin

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13519 pGEMHE-mEGFP-INCENP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13519_B0Jup1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: mVenus-M_F
          Region: insert mid (mEGFP 3', INCENP 5')
          Sequence file: RDB13519_B0Jua.seq check
          >13519_13519_B0Ju_1_mVenus-M_F_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNANNNGGN NTCNNGGTGN NCTTCNNGAN CCGCCNCNAC ATCGAGGACG
             61 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
            121 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
            181 AGCGCGATCA CATGGTCCTT AAGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
            241 ACGAGCTGTA CAAGTACTCA GATCTCGAGC TCAAGCTTCG AATTCTGCAG TCGACGGCCA
            301 CCATGGGGAC GACGGCCCCA GGGCCCATTC ACCTGCTGGA GCTATGTGAC CAGAAGCTCA
            361 TGGAGTTTCT CTGCAACATG GATAATAAGG ACTTGGTGTG GCTTGAGGAG ATCCAAGAGG
            421 AGGCCGAGCG CATGTTCACC AGAGAATTCA GCAAAGAGCC AGAGCTGATG CCCAAAACAC
            481 CTTCTCAGAA GAACCGACGG AAGAAGAGAC GGATTTCTTA TGTTCAGGAT GAAAACAGAG
            541 ATCCCATCAG GAGAAGGTTA TCCCGCAGAA AGTCTCGGAG CAGCCAGCTG AGCTCCCGAC
            601 GCCTCCGCAG CAAGGACAGT GTAGAGAAGC TGGCTACAGT GGTCGGGGAG AACGGCTCCG
            661 TTCTGCGGCG TGTGACCCGT GCTGCGGCTG CAGCTGCCGC GGCTACCATG GCATTGGCTG
            721 CACCTTCTTC ACCCACCCCT GANTCTCCCA CGATGCTGAC TAANAAGCCC GANGATAACC
            781 ACACCCAGNG CCAGCTGGTG CCTGTGGNGG AGATCGGNAT CAGTGAGCGN CNGAATGCTG
            841 ANCANCATGT CACCCAGCTC ATGTCCNCCG ANCCTCNGCC CCGCANNCTG NCCCCGANTC
            901 CNGCTTCNNN NNNNGCTNCN ANCNTCCCAG GGNATCCCGA NNTCNNANGA NGNNNCNNNN
            961 NCNTANGNNG NNNAANGNCN NNNANACTNN NNNCCANNNN NNNGANNNNC NNNNNNNNNN
          //
          Primer: RDB13509#1
          Region: insert mid (Xenopus globin 3'-UTR, INCENP 3')
          Sequence file: RDB13519_B0Jub.seq check
          >13519_13519_B0Ju_1_RDB13509#1_G03_21_ABI24.ab1
              1 NNNNNNNNNN NNNNANNNGT NNNNNGGTAT TATGTAGCTT AGAGACTCCA TTCGGGTGTT
             61 CTTGAGGCTG GTTTAGTGGT AACCAGATCA AGCTATCATT TATCTAGAGC GGCCGCTTTA
            121 CTTGTACAGG ATCCGCCTAG TGCTTCTTCA GGCTGTAGGC CAGGCTGCTG GGGACCCTGG
            181 CGCCCTGCAG GGGCGGTGAG TTCCAGACAG CAGAGCTGGT GCGCTTGTGA TAGCGGGGCT
            241 TGCTCTTCTT GAAGATATCC TCCAAGTCCA GTGGGAGAAT GGTTCCAAAG AGCTCCAGAA
            301 GGTTCGGTGG GTGGTAGTAC TGGTGAATGA TAGCCTGGCT GAGCGGGGTG CCTCGGGCCC
            361 AGGTGGGGAT GGGCTTCCGG GGATGGGCCT CATCATCGGT GGAGTCGTCG CTATTCAGAT
            421 CCATCCCGTA GTTATCTGGG TTGATCTTGG GAGGGGCCCT GTGCCCTTGC GGAGTCATCT
            481 GATATGAGGT ACAAGCTGGA GACTGCACGT CCACAGTCAC ATTCAGGGCC TTGCTGGCCC
            541 CTGCTGCCTC CTTGGCTTTC TTCTCTTGCT CCTCCTGCAG CTGCCGCTCA GCCAGACGCT
            601 GCTGCTCTTC CTTCTTCTTC TTCTCCTCCA GTTCCCTCTG CAGCTGCTCC TTCTGCAGCC
            661 GCANGGCCTT CTCCCGCTCC TGCAGCTNCC TCTCNGNCCT NNNCCGCTCC NGNTNCCGNN
          721 NANNCNNNNN TCNNNNNNNN NN
          //
          Primer: HB-NOS_R
          Region: T7 pro, insert 5' (Xenopus globin 5'-UTR, mEGFP 5')
          Sequence file: RDB13519_B0Juc.seq check
          >13519_13519_B0Ju_1_HB-NOS_R_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNGNGNNN NNTTGGNNNN NCCNGGGTTT TCCCAGTCNC GACGTTGTAA
             61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTAATTC GAGCTCGGTA
            121 CCCAGCTTGC TTGTTCTTTT TGCAGAAGCT CAGAATAAAC GCTCAACTTT GGCAGATCAA
            181 TTCCCCGGTG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
            241 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
            301 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
            361 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
            421 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
            481 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
            541 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
            601 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
            661 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
            721 CAACATCGAG GANGGCAGCG TGCAGCTCGC CGACCACTAC NAGCAGANCA CCCCCATCGG
            781 CGACNGCCCC GTGCTGCTGC CCGACNACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA
            841 AGACCCCAAC GAGAANCGCG ATNACATGGT NCTTAAGGAG TTCGTGANNG CCGCCGGGNT
            901 CACTCTCGGC ANGGNNGANC NGTACNAGTA CTCANATCTC GAGCTNNNGC TTCNNNNTCN
            961 GCANNCNANN NNCNNCNNNG NNNNNGANNG NNNNAGGGNC CATNNNNCNN GCNNNNNNNN
          1021 NN
          //
          Primer: SV40pA3'element Reverse
          Region: SP6 pro
          Sequence file: RDB13519_B0Jud.seq check
          >13519_13519_B0Ju_1_SV40pA3'elementReverse_H03_24_ABI24.ab1
              1 NNNNNNNGNN NNNNNNNNGN NNNNGCCNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
             61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
            121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
            181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
            241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
            301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
            361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
            421 GACCATGATT ACGCCAAGCT ATTTAGGTGA CACTATAGAA TACTCAAGCT AGATTAATTA
          481 ATTTAAATCG CCGGCGGCGC GCCTGCAGGG GGGGGGGGGG NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          2022.07.13

          GNP_filter3_RDBDEP_html_220713.pl