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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEMHE-mHec1-sfGFP

Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.

Catalog number RDB13516
Resource name pGEMHE-mHec1-sfGFP
Clone info. Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. Fused with sfGFP.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Ndc80 cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |
Other clones in our bank

External Database
mouse Ndc80 Aequorea victoria GFP
    Xenopus laevis beta-globin

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13516 pGEMHE-mHec1-sfGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Visualization of Organelles (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13516_A5Isp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13516_A5Isa.seq check
          >D02675A8_A5Is_4_M13(-40)_E03_15.ab1
              1 AGCCAGCTAA TGTAATACGA CTCACTATAG GGCGAATTAA TTCGAGCTCG GTACCCAGCT
             61 TGCTTGTTCT TTTTGCAGAA GCTCAGAATA AACGCTCAAC TTTGGCAGAT CAATTCCCCG
            121 GTGCTAGCGC TACCGGTATG AAGCGCAGTT CAGTTTCCAC CTGTGGTGCT GGCCGCCTCT
            181 CTATGCAGGA GTTAAGGACC CTGGACCTCA ATAAGCCAGG CCTTTATACC CCTCAAACCA
            241 AAGAAAGATC AACCTTTGGA AAGCTGAGTA CACACAAACC GACATCGGAA AGAAAAGTCT
            301 CAATATTTGG GAAAAGGACT AGCGGACATG GATCCAGGAA TAGTCAACTT GGTATATTTT
            361 CCAGTTCTGA AAAAATCAAG GACCCAAGAC CACTTAATGA CAAAGCATTC ATTCAGCAGT
            421 GTATTCGACA ACTCTATGAG TTTCTTACAG AAAACGGTTA TGTGTATAGT GTATCCATGA
            481 AGTCTCTGCA AGCTCCATCC ACTAAAGAGT TCCTAAAGAT CTTCGCCTTT CTTTATGGCT
            541 TTCTGTGCCC GTCGTATGAA CTTCCTGGTA CAAAATGTGA AGAAGAGGTC CCAAGAATTT
            601 TTAAAGCACT TGGGTATCCC TTCACACTGT CCAAGAGCTC CATGTATACA GTGGGAGCCC
            661 CTCACACGTG GCCTCACATC GTGGCTGCCT TGGTGTGGCT CATAGACTGC ATCAAGATTG
            721 ATACTGCCAT GAAAGAAAGC TCACCTTTAT TTGATGATGG GCAGCTCTGG GGAGAAGAGA
            781 CTGAAGATGG AATTAAACAC AATAAGTTGT TTTTGGAGTA CACCAAAAAG TGCTATGAGA
            841 AGTTCATGAC CGGGGCCGAC AGCTTTGAAG AAGAGGATGC TGAGCTGCAG GCGAGGCTGA
            901 AAGGACTTGT ACAAGGTAGA TGCGTCTAAG CTGGAGTCAC TCGAAGCAGA AAACAAAGCA
            961 CTAAATGAAC AGATTGCAAG ACTGGAGGAG GAAAGAGAAA GAGAACCGAA ACCGTCTGAT
           1021 GTCATTGAGA ACTGAAAGCG TCCTTACAAG CAGATGTCAA AACTATAAAG CATACATGAA
           1081 GCCACCTGAG TCTCATTAGG CGTCTTGAAC AGATTCGATA GGTCCTGAAT GAAGAATGTT
          1141 AAGAAGTTAG AACAAGGAAA GT
          //
          Primer: EGFP-N
          Sequence file: RDB13516_A5Isb.seq check
          >D02675A8-A5Is_4_EGFP_N_G03_21.ab1
              1 GGGTACCGGG GTGACGCCTC TCGCCCTTGC TCACCATGGT GGCGACCGGT CCTTTGTCGG
             61 GAGCCTTAAG TTGGGCAGCA TTTCTCTTAT ACTTCTCTGC CATCTCCCTG ATGTTTTCCA
            121 GGAGATCTTC AGACATGAAT TCTTCATACT CTCTGTCGGC TTTAGCATTC TCCTCTTCAA
            181 GATGTTTCTC CAAAGACCCT ACGTGTGTGG CGACCATCTC CAAAAGACGT TGTAAGTTGT
            241 TTTCCACCTT TCTTCTTTCT TCAGTTGTGG TCTTCACAGT TAGCTGGTAT TCCCGCTGGA
            301 CGGCGTCCAA CTCATCCATG GCCTCGCTGA GGCCATCGTT CACCCCGCTC TCCAGCAGGT
            361 GCTTGTGTTT CTCCAGGGAC TCAAGCTCAC TGGCACTCTT CTTGTCTTTT TCCTCAGCTT
            421 CCTTCACTGC CTGCTGGTGA AGGTCATCCA GTTTCTGAAT CTCCTCCTTC AGCATCCTCA
            481 CAGTGTTCTT GCTTTCCGTT TTCATGGTGT TCAGTTGTTC TAAAGTATCC TCCAGATGCC
            541 TCTTTTTATT CAGAGCTTTG TTAATTTCTT CTTCGCTTTC ATTCAAGAGC TCTTTGAGCG
            601 GTGCATACAC TTGAGTCCTG TATTTGACAA GGCAGTTGGC ACCCGCCTCA GGATTAAACT
            661 TAATTTCAAA GTCGTAACCT TTGGAATTCT CAGCACCTTT GGGGGATAAG CTTTAATTTT
            721 CCTAGCCAAC TTGTGGTACT CCGCTAGCTG CGCTTCAATC GCCTCTTTGC CTCTTGCGTA
            781 TTTTAATTCT TTCATTCCAC ATCTGTTGCT GTTTCGGCCT TCCAGGTCTT TTGGTTTAAT
            841 TTATTAAATA GTCTGCTGCA ATTTCATTTT TTCCTCATGA ATTTATTTCT CTTCAAATGT
            901 CCAGCGACCT GAAATACCTT CTGGGTTATT CAACGATACT TCTGGTATTC CAAGTGGTTT
          961 TCCCTGTTTT AACAG
          //
          Primer: RDB13509#1
          Sequence file: RDB13516_A5Isc.seq check
          >D02675A8-A5Is_4_RDB13509#1_H03_24.ab1
              1 CGGGCATTTT GTAAGTGTAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT
             61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTTACTT
            121 GTACAGGATC CCGGGCCGTC GACTGCAGAA TTCGAAGCTT GAGCTCGAGA TCTGAGTCCG
            181 GCCGGACTTG TACAGCTCGT CCATGCCGTG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
            241 AAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCACGGACT GGGTGCTCAG
            301 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
            361 GTCGGCGAGC TGCACGCTGC CGTCCTCCAC GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
            421 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGAAGT TGTACTCCAG
            481 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
            541 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GCCGCGGGTC TTGTAGGTGC CGTCGTCCTT
            601 GAAGCTGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
            661 GCGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC
            721 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GTTGGTGCAG ATGAACTTCA GGGTCAGCTT
            781 GCCGTTGGTG GCATCGCCCT CGCCCTCGCC GCGCACGCTG AACTTGTGGC CGTTTACGTC
            841 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
            901 CATGGTGGCG ACCGGTCCTT TGTCGGGAGC CTTAAGTTGG GCAGCATTTC TCTTATACTT
            961 CTCTGCCATC TCCCTGATGT TTTCCCAGGG AGATCTTCAG ACATGAAATT TCTTTCATAC
           1021 TCTCTGTCGG CTTTAGCATT CTCGTCTTCA GATGTTTCTC CAAGACCTAC GTGTGTGGCG
          1081 AACAATCTCC AAAAAGAACC G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl