Resource data sheet
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pGEMHE-mHec1-sfGFP

Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.

Catalog number RDB13516
Resource name pGEMHE-mHec1-sfGFP
Clone info. Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte. Fused with sfGFP.
pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Ndc80 cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor Kitajima, Tomoya |
 
Other clones in our bank mouse Ndc80 (NCBI Gene 67052) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13516 pGEMHE-mHec1-sfGFP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Organelle marker (English text)

References

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13516_A5Isp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB13516_A5Isa.seq
>D02675A8_A5Is_4_M13(-40)_E03_15.ab1
    1 AGCCAGCTAA TGTAATACGA CTCACTATAG GGCGAATTAA TTCGAGCTCG GTACCCAGCT
   61 TGCTTGTTCT TTTTGCAGAA GCTCAGAATA AACGCTCAAC TTTGGCAGAT CAATTCCCCG
  121 GTGCTAGCGC TACCGGTATG AAGCGCAGTT CAGTTTCCAC CTGTGGTGCT GGCCGCCTCT
  181 CTATGCAGGA GTTAAGGACC CTGGACCTCA ATAAGCCAGG CCTTTATACC CCTCAAACCA
  241 AAGAAAGATC AACCTTTGGA AAGCTGAGTA CACACAAACC GACATCGGAA AGAAAAGTCT
  301 CAATATTTGG GAAAAGGACT AGCGGACATG GATCCAGGAA TAGTCAACTT GGTATATTTT
  361 CCAGTTCTGA AAAAATCAAG GACCCAAGAC CACTTAATGA CAAAGCATTC ATTCAGCAGT
  421 GTATTCGACA ACTCTATGAG TTTCTTACAG AAAACGGTTA TGTGTATAGT GTATCCATGA
  481 AGTCTCTGCA AGCTCCATCC ACTAAAGAGT TCCTAAAGAT CTTCGCCTTT CTTTATGGCT
  541 TTCTGTGCCC GTCGTATGAA CTTCCTGGTA CAAAATGTGA AGAAGAGGTC CCAAGAATTT
  601 TTAAAGCACT TGGGTATCCC TTCACACTGT CCAAGAGCTC CATGTATACA GTGGGAGCCC
  661 CTCACACGTG GCCTCACATC GTGGCTGCCT TGGTGTGGCT CATAGACTGC ATCAAGATTG
  721 ATACTGCCAT GAAAGAAAGC TCACCTTTAT TTGATGATGG GCAGCTCTGG GGAGAAGAGA
  781 CTGAAGATGG AATTAAACAC AATAAGTTGT TTTTGGAGTA CACCAAAAAG TGCTATGAGA
  841 AGTTCATGAC CGGGGCCGAC AGCTTTGAAG AAGAGGATGC TGAGCTGCAG GCGAGGCTGA
  901 AAGGACTTGT ACAAGGTAGA TGCGTCTAAG CTGGAGTCAC TCGAAGCAGA AAACAAAGCA
  961 CTAAATGAAC AGATTGCAAG ACTGGAGGAG GAAAGAGAAA GAGAACCGAA ACCGTCTGAT
 1021 GTCATTGAGA ACTGAAAGCG TCCTTACAAG CAGATGTCAA AACTATAAAG CATACATGAA
 1081 GCCACCTGAG TCTCATTAGG CGTCTTGAAC AGATTCGATA GGTCCTGAAT GAAGAATGTT
1141 AAGAAGTTAG AACAAGGAAA GT
//
Primer: EGFP-N RDB13516_A5Isb.seq
>D02675A8-A5Is_4_EGFP_N_G03_21.ab1
    1 GGGTACCGGG GTGACGCCTC TCGCCCTTGC TCACCATGGT GGCGACCGGT CCTTTGTCGG
   61 GAGCCTTAAG TTGGGCAGCA TTTCTCTTAT ACTTCTCTGC CATCTCCCTG ATGTTTTCCA
  121 GGAGATCTTC AGACATGAAT TCTTCATACT CTCTGTCGGC TTTAGCATTC TCCTCTTCAA
  181 GATGTTTCTC CAAAGACCCT ACGTGTGTGG CGACCATCTC CAAAAGACGT TGTAAGTTGT
  241 TTTCCACCTT TCTTCTTTCT TCAGTTGTGG TCTTCACAGT TAGCTGGTAT TCCCGCTGGA
  301 CGGCGTCCAA CTCATCCATG GCCTCGCTGA GGCCATCGTT CACCCCGCTC TCCAGCAGGT
  361 GCTTGTGTTT CTCCAGGGAC TCAAGCTCAC TGGCACTCTT CTTGTCTTTT TCCTCAGCTT
  421 CCTTCACTGC CTGCTGGTGA AGGTCATCCA GTTTCTGAAT CTCCTCCTTC AGCATCCTCA
  481 CAGTGTTCTT GCTTTCCGTT TTCATGGTGT TCAGTTGTTC TAAAGTATCC TCCAGATGCC
  541 TCTTTTTATT CAGAGCTTTG TTAATTTCTT CTTCGCTTTC ATTCAAGAGC TCTTTGAGCG
  601 GTGCATACAC TTGAGTCCTG TATTTGACAA GGCAGTTGGC ACCCGCCTCA GGATTAAACT
  661 TAATTTCAAA GTCGTAACCT TTGGAATTCT CAGCACCTTT GGGGGATAAG CTTTAATTTT
  721 CCTAGCCAAC TTGTGGTACT CCGCTAGCTG CGCTTCAATC GCCTCTTTGC CTCTTGCGTA
  781 TTTTAATTCT TTCATTCCAC ATCTGTTGCT GTTTCGGCCT TCCAGGTCTT TTGGTTTAAT
  841 TTATTAAATA GTCTGCTGCA ATTTCATTTT TTCCTCATGA ATTTATTTCT CTTCAAATGT
  901 CCAGCGACCT GAAATACCTT CTGGGTTATT CAACGATACT TCTGGTATTC CAAGTGGTTT
961 TCCCTGTTTT AACAG
//
Primer: RDB13509#1 RDB13516_A5Isc.seq
>D02675A8-A5Is_4_RDB13509#1_H03_24.ab1
    1 CGGGCATTTT GTAAGTGTAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT
   61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTTACTT
  121 GTACAGGATC CCGGGCCGTC GACTGCAGAA TTCGAAGCTT GAGCTCGAGA TCTGAGTCCG
  181 GCCGGACTTG TACAGCTCGT CCATGCCGTG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
  241 AAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCACGGACT GGGTGCTCAG
  301 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
  361 GTCGGCGAGC TGCACGCTGC CGTCCTCCAC GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
  421 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGAAGT TGTACTCCAG
  481 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
  541 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GCCGCGGGTC TTGTAGGTGC CGTCGTCCTT
  601 GAAGCTGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
  661 GCGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC
  721 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GTTGGTGCAG ATGAACTTCA GGGTCAGCTT
  781 GCCGTTGGTG GCATCGCCCT CGCCCTCGCC GCGCACGCTG AACTTGTGGC CGTTTACGTC
  841 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
  901 CATGGTGGCG ACCGGTCCTT TGTCGGGAGC CTTAAGTTGG GCAGCATTTC TCTTATACTT
  961 CTCTGCCATC TCCCTGATGT TTTCCCAGGG AGATCTTCAG ACATGAAATT TCTTTCATAC
 1021 TCTCTGTCGG CTTTAGCATT CTCGTCTTCA GATGTTTCTC CAAGACCTAC GTGTGTGGCG
1081 AACAATCTCC AAAAAGAACC G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

2018.12.03

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