Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEMHE-mHec1-sfGFP

Labeling outer kinetochore. Plasmid for in vitro transcription of mouse Hec1 (Ndc80) in mouse oocyte.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13516_A5Isp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 () Sequence file: RDB13516_A5Isa.seq
>D02675A8_A5Is_4_M13(-40)_E03_15.ab1     1 AGCCAGCTAA TGTAATACGA CTCACTATAG GGCGAATTAA TTCGAGCTCG GTACCCAGCT    61 TGCTTGTTCT TTTTGCAGAA GCTCAGAATA AACGCTCAAC TTTGGCAGAT CAATTCCCCG   121 GTGCTAGCGC TACCGGTATG AAGCGCAGTT CAGTTTCCAC CTGTGGTGCT GGCCGCCTCT   181 CTATGCAGGA GTTAAGGACC CTGGACCTCA ATAAGCCAGG CCTTTATACC CCTCAAACCA   241 AAGAAAGATC AACCTTTGGA AAGCTGAGTA CACACAAACC GACATCGGAA AGAAAAGTCT   301 CAATATTTGG GAAAAGGACT AGCGGACATG GATCCAGGAA TAGTCAACTT GGTATATTTT   361 CCAGTTCTGA AAAAATCAAG GACCCAAGAC CACTTAATGA CAAAGCATTC ATTCAGCAGT   421 GTATTCGACA ACTCTATGAG TTTCTTACAG AAAACGGTTA TGTGTATAGT GTATCCATGA   481 AGTCTCTGCA AGCTCCATCC ACTAAAGAGT TCCTAAAGAT CTTCGCCTTT CTTTATGGCT   541 TTCTGTGCCC GTCGTATGAA CTTCCTGGTA CAAAATGTGA AGAAGAGGTC CCAAGAATTT   601 TTAAAGCACT TGGGTATCCC TTCACACTGT CCAAGAGCTC CATGTATACA GTGGGAGCCC   661 CTCACACGTG GCCTCACATC GTGGCTGCCT TGGTGTGGCT CATAGACTGC ATCAAGATTG   721 ATACTGCCAT GAAAGAAAGC TCACCTTTAT TTGATGATGG GCAGCTCTGG GGAGAAGAGA   781 CTGAAGATGG AATTAAACAC AATAAGTTGT TTTTGGAGTA CACCAAAAAG TGCTATGAGA   841 AGTTCATGAC CGGGGCCGAC AGCTTTGAAG AAGAGGATGC TGAGCTGCAG GCGAGGCTGA   901 AAGGACTTGT ACAAGGTAGA TGCGTCTAAG CTGGAGTCAC TCGAAGCAGA AAACAAAGCA   961 CTAAATGAAC AGATTGCAAG ACTGGAGGAG GAAAGAGAAA GAGAACCGAA ACCGTCTGAT  1021 GTCATTGAGA ACTGAAAGCG TCCTTACAAG CAGATGTCAA AACTATAAAG CATACATGAA  1081 GCCACCTGAG TCTCATTAGG CGTCTTGAAC AGATTCGATA GGTCCTGAAT GAAGAATGTT 1141 AAGAAGTTAG AACAAGGAAA GT //
Primer: EGFP-N Sequence file: RDB13516_A5Isb.seq
>D02675A8-A5Is_4_EGFP_N_G03_21.ab1     1 GGGTACCGGG GTGACGCCTC TCGCCCTTGC TCACCATGGT GGCGACCGGT CCTTTGTCGG    61 GAGCCTTAAG TTGGGCAGCA TTTCTCTTAT ACTTCTCTGC CATCTCCCTG ATGTTTTCCA   121 GGAGATCTTC AGACATGAAT TCTTCATACT CTCTGTCGGC TTTAGCATTC TCCTCTTCAA   181 GATGTTTCTC CAAAGACCCT ACGTGTGTGG CGACCATCTC CAAAAGACGT TGTAAGTTGT   241 TTTCCACCTT TCTTCTTTCT TCAGTTGTGG TCTTCACAGT TAGCTGGTAT TCCCGCTGGA   301 CGGCGTCCAA CTCATCCATG GCCTCGCTGA GGCCATCGTT CACCCCGCTC TCCAGCAGGT   361 GCTTGTGTTT CTCCAGGGAC TCAAGCTCAC TGGCACTCTT CTTGTCTTTT TCCTCAGCTT   421 CCTTCACTGC CTGCTGGTGA AGGTCATCCA GTTTCTGAAT CTCCTCCTTC AGCATCCTCA   481 CAGTGTTCTT GCTTTCCGTT TTCATGGTGT TCAGTTGTTC TAAAGTATCC TCCAGATGCC   541 TCTTTTTATT CAGAGCTTTG TTAATTTCTT CTTCGCTTTC ATTCAAGAGC TCTTTGAGCG   601 GTGCATACAC TTGAGTCCTG TATTTGACAA GGCAGTTGGC ACCCGCCTCA GGATTAAACT   661 TAATTTCAAA GTCGTAACCT TTGGAATTCT CAGCACCTTT GGGGGATAAG CTTTAATTTT   721 CCTAGCCAAC TTGTGGTACT CCGCTAGCTG CGCTTCAATC GCCTCTTTGC CTCTTGCGTA   781 TTTTAATTCT TTCATTCCAC ATCTGTTGCT GTTTCGGCCT TCCAGGTCTT TTGGTTTAAT   841 TTATTAAATA GTCTGCTGCA ATTTCATTTT TTCCTCATGA ATTTATTTCT CTTCAAATGT   901 CCAGCGACCT GAAATACCTT CTGGGTTATT CAACGATACT TCTGGTATTC CAAGTGGTTT 961 TCCCTGTTTT AACAG //
Primer: RDB13509#1 Sequence file: RDB13516_A5Isc.seq
>D02675A8-A5Is_4_RDB13509#1_H03_24.ab1     1 CGGGCATTTT GTAAGTGTAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT    61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTTACTT   121 GTACAGGATC CCGGGCCGTC GACTGCAGAA TTCGAAGCTT GAGCTCGAGA TCTGAGTCCG   181 GCCGGACTTG TACAGCTCGT CCATGCCGTG AGTGATCCCG GCGGCGGTCA CGAACTCCAG   241 AAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCACGGACT GGGTGCTCAG   301 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG   361 GTCGGCGAGC TGCACGCTGC CGTCCTCCAC GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT   421 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGAAGT TGTACTCCAG   481 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT   541 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GCCGCGGGTC TTGTAGGTGC CGTCGTCCTT   601 GAAGCTGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG   661 GCGCTTCATG TGGTCGGGGT AGCGGCTGAA GCACTGCACG CCGTAGGTCA GGGTGGTCAC   721 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GTTGGTGCAG ATGAACTTCA GGGTCAGCTT   781 GCCGTTGGTG GCATCGCCCT CGCCCTCGCC GCGCACGCTG AACTTGTGGC CGTTTACGTC   841 GCCGTCCAGC TCGACCAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC   901 CATGGTGGCG ACCGGTCCTT TGTCGGGAGC CTTAAGTTGG GCAGCATTTC TCTTATACTT   961 CTCTGCCATC TCCCTGATGT TTTCCCAGGG AGATCTTCAG ACATGAAATT TCTTTCATAC  1021 TCTCTGTCGG CTTTAGCATT CTCGTCTTCA GATGTTTCTC CAAGACCTAC GTGTGTGGCG 1081 AACAATCTCC AAAAAGAACC G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl