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pGEMHE-mBubR1-3A

Plasmid for in vitro transcription of mouse Bub1b in mouse oocyte.

Catalog number RDB13514
Resource name pGEMHE-mBubR1-3A
Alternative name pGEMHE-mEGFP-BubR1-3A
Clone info. Plasmid for in vitro transcription of mouse Bub1b tagged with mEGFP in mouse oocyte. Phosphodeficient mutation at S659A, S665A, T669A in KARD domain.
pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Bub1b cDNA Xenopus laevis beta-globin cDNA
Depositor Kitajima, Tomoya |
 
Other clones in our bank mouse Bub1b (NCBI Gene 12236) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13514 pGEMHE-mBubR1-3A Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

2019.07.20

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