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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEMHE-mBubR1-WT

Plasmid for in vitro transcription of mouse Bub1b in mouse oocyte.

Catalog number RDB13513
Resource name pGEMHE-mBubR1-WT
Alternative name pGEMHE-mEGFP-BubR1
Clone info. Plasmid for in vitro transcription of mouse Bub1b tagged with mEGFP in mouse oocyte.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Bub1b cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |
Other clones in our bank

External Database
mouse Bub1b Xenopus laevis beta-globin

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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          RDB13513 pGEMHE-mBubR1-WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13513_A6Aep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB13513_A5Isa.seq check
          >D02675A5-A5Is_4_T7_20mer_B02_05.ab1
              1 TACCCCGGTA CCAGCTTGCT TGTTCTTTTT GCAGAAGCTC AGAATAAACG CTCAACTTTG
             61 GCAGATCAAT TCCCCGGTGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA
            121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
            181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
            241 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
            301 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
            361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
            421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
            481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
            541 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
            601 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
            661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
            721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTTAAGGAGT TCGTGACCGC
            781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAC TCAGATCTCG AGCTCAAGCT
            841 TCGAATTCTG CAGTCGACGA TGGCGGAGGC GAGTGAAGCC ATGTGCCTGG AGGGAGCAGA
            901 GTGGGAGCTG AGTAAAGAAA ACATACAGCC CTTTACGGCA CGGGCGGGTC ATGTCCACAC
            961 TTCAGGAGCT TTGGGCAAGC AGAGTCAGCC CGGCCACACT GCTCTGCAGC AGCAGAAACC
           1021 GGGCAATTTT GATCTGAAAT CCGCTTTTAC TCTGAGATGA ACCCTCTGGT ATGTGGACCG
          1081 ATATATATGG GACAGAAACG GAACTTAA
          //
          Primer: mEGFP-down2
          Sequence file: RDB13513_A5Isb.seq check
          >D02675A5_A5Is_4_mEGFP-down2_E12_15.ab1
              1 CTGAATTTGC AGTCGACGAT GGCGGAGGCG AGTGAAGCCA TGTGCCTGGA GGGAGCAGAG
             61 TGGGAGCTGA GTAAAGAAAA CATACAGCCC TTACGGCACG GGCGGGTCAT GTCCACACTT
            121 CAGGGAGCTT TGGCAAAGCA AGAGTCAGCC GGCCACACTG CTCTGCAGCA GCAGAAACGG
            181 GCATTTGAAT CTGAAATCCG CTTTTACTCT GGAGATGACC CTCTGGATGT GTGGGACAGA
            241 TATATTAATT GGACAGAACA GAACTACCCT CAAGGGGGGA AAGAGAGTAA CATGTCAGCG
            301 TTAGTGGAGA GAGCGATAGA AGCACTCCAA GGAGAGACGC GCTATTATAA TGACCCCCGC
            361 TTTCTCAGTC TCTGGATCAA ATTGGGACAT TTGTGCAATG AACCTTTGGA TATGTACAGC
            421 TATTTACAAA GCCAAGGAAT TGGCGTTTCC CTTGCCCAGT TCTATATTTC ATGGGCTGAA
            481 GAATACGAAG CTAGAGAAAA TTTCAAGAAA GCGGACATAA TATTCCAGGA AGGGATTGAA
            541 CGCAAGGCTG AGCCCCTGGA CAGACTGCAG TCCCAGCACA GACAGTTCCA GTCTCGAGTG
            601 TCGCGACAAG CTTTCTTGGC CCTTGGGAAT GAAGAGGAGA GCTTTGGAGC CTTCTGAACC
            661 ACAGAGAGCT CGCTAGCTGA GCTGAAGCAA AGGAAGAAGA TGGCCAGAGC GCCATCAGCG
            721 TGTCGAGGTG CTCTGAAAGC TCCAGTCGAG CGAAGGTCTA AATGCAGTTC CCCAGCAGTA
            781 CACGGTAATC GCAGATCACC GTTTTGATGA ATGGCGAAAC CCGGCTCTAA CGATTATAGC
            841 CTTGTACCCA CCATGGATGC ACCCTTGTGC CAGGCAAGGA AAGACTCACA GTCTAGGGAA
            901 CAATGGCCGG GAACAGCCTA GAATCAGCCT GGGAGCGAAT CCAGGTCCAG TACGATGAAG
            961 GCCCCAACTG AACCTGATGC TATACCGTTC CCGACCGGGA CGACCTTGGA GTCACTCCGT
          1021 GAGGAGTCGG AAACT
          //
          Primer: RDB13509#1
          Sequence file: RDB13513_A5Isc.seq check
          >D02675A5-A5Is_4_RDB13509#1_D02_11.ab1
              1 CGGGCCTTTT GTAGTGTAAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT
             61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTAGGCA
            121 GGGCTCTGGG AGCCGCCTGG CTGCTTGTCT TGCTGAGTGA GCAAAGCTTC CGGGCTGATT
            181 GTCTTCCCCA GCTTCCACAG GGCTCTGTTC AGGTGGCTAT GGAATGTGGC ATCAAAAGCC
            241 CCACCCATTT CTGCTGCCAG CTCCCCCAGA ACAGACACTG TGGACTTGTC ACTGGCATTC
            301 AGAATCCGCA CAAAGAATTT ATTCCACAAT TCACTGTCTT TTAGCTCAGA GGTGCTCTGG
            361 CTAAGTTTCC AGAGGAGTCC ATCCCAGAAG ACATGGAGGT GCTCCTTGAA CAGGAGTAAG
            421 TGCGCTAGGT CTGCTATACC CAACAGATCT ACATGGTAGG GAGAAGAACA GTTAGCCAGG
            481 ATCTTTTGTC CTTCCAGGAT CTGTGCAGTC CGAAAGCCAC TATAGGCAAA CGCATCCAGC
            541 TGCACCCTCA GGTCAACACT GTAGGAGAAG TCCATGATCC TCACAGCGTG ATCGTCCTTA
            601 TTTACATAGT CATAGGGGTC GTGGATTCTG TTTCGTAGGA TCAGACTCCG TGGACTCAAG
            661 TCTCCGTGTA CTATTTCAGC TCTGTGTAGC TTCTCCACGA TTGTCAAGAG GTTGTAAATA
            721 ATCAACACTA TTATTTCATG AGTAACAAAT TCGCTGTGTT GGAGAAGATT CTGAAGGGTG
            781 GAGCAGTTTA TATACTGGTA CCAAACAACA TGGCCATCTT GGTACTGACA GCAGCTGCAA
            841 AGCTGGTCAT AGTCCTCATT CAGACGCTCC TTCAACTTGA GGTTGATATA AAAAATCCCA
            901 CGGGATAGGC TGAGATGATG CCTTTATCAT GGGTTAGCTC TGCAGAGCTT CTTTGGTGCC
            961 ACCCCAGAAT AACCCTGTAA TCGTCACATG TTTAGTGCTC TTGCTTGATG ACGTTAATCT
           1021 CAGACCCTAA CTCAATCTCG ACCCTATTTC AAGCACAGCA TCGGTTCGGA TCATCCACAG
          1081 AAAACTTTCG CAAAAGTCAA CT
          //
          Primer: RDB13513#1
          Sequence file: RDB13513_A5Isd.seq check
          >D02675A5-A5Is_4_RDB13513#1_E02_14.ab1
              1 CCGTTACCGA GAGGGAATGT TTGCAGGAAA GAGGATCTGG ATGGGAAAGG CCGGCCGAGG
             61 CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA ATTATTGAAG
            121 AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA GCTCCCAGTA
            181 CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT GACGGGGCAG
            241 AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA AAATCCCTAC
            301 TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT GTGCTGGAAA
            361 TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG CACCTAACAT
            421 GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA ACCATGATAA
            481 AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG AAGGAGCGTC
            541 TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC CATGTTGTTT
            601 GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC GAATTTGTTA
            661 CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG AAGCTACACA
            721 GAGCTGAAAA TAGTACACGG AGACTTGAGT CCACGGAGTC TGATCCTACG AAACAGAATC
            781 CACGACCCCT ATGACTATGT AAATAAAGGA CGATCACGCT GTGAGGATCA TGGACTTCTC
            841 CTACAGTGTT GACCCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC TTTCGGACTT
            901 GCACAGATCC TGGAAAGGAC AAAAGATCCC TGGCTAACTG TTCTTCCTCC CTACCAATGT
            961 AGATCTGTTT GGGGTATAGC CAGACCCTAG CCGCAACTTA CTTCCCTGTT CCAAGGGAGC
           1021 CACCCTTCCA TGTTCTTTCC TTGGGAATGG ACCTTCCTTC TGGGAAACCT TTAGCCCAGA
           1081 AGCCAACCCT TCTGAAGCTT AAAAAGACAC AGTTGAAAAT TTGTGGGAAT TAAATTCCTT
          1141 TTGTTGCCGA TATTCTGAAA TGACCAAGGT GGGAAACAAG AAGATTTTCC CA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl