Resource data sheet
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pGEMHE-mBubR1-WT

Plasmid for in vitro transcription of mouse Bub1b in mouse oocyte.

Catalog number RDB13513
Resource name pGEMHE-mBubR1-WT
Alternative name pGEMHE-mEGFP-BubR1
Clone info. Plasmid for in vitro transcription of mouse Bub1b tagged with mEGFP in mouse oocyte.
pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name mouse Bub1b cDNA Xenopus laevis beta-globin cDNA
Depositor Kitajima, Tomoya |
 
Other clones in our bank mouse Bub1b (NCBI Gene 12236) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
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RDB13513 pGEMHE-mBubR1-WT DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13513_A6Aep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13513_A5Isa.seq
>D02675A5-A5Is_4_T7_20mer_B02_05.ab1
    1 TACCCCGGTA CCAGCTTGCT TGTTCTTTTT GCAGAAGCTC AGAATAAACG CTCAACTTTG
   61 GCAGATCAAT TCCCCGGTGC TAGCGCTACC GGTCGCCACC ATGGTGAGCA AGGGCGAGGA
  121 GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA
  181 GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT
  241 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA
  301 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC
  361 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  421 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  481 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
  541 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
  601 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  661 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
  721 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTTAAGGAGT TCGTGACCGC
  781 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAC TCAGATCTCG AGCTCAAGCT
  841 TCGAATTCTG CAGTCGACGA TGGCGGAGGC GAGTGAAGCC ATGTGCCTGG AGGGAGCAGA
  901 GTGGGAGCTG AGTAAAGAAA ACATACAGCC CTTTACGGCA CGGGCGGGTC ATGTCCACAC
  961 TTCAGGAGCT TTGGGCAAGC AGAGTCAGCC CGGCCACACT GCTCTGCAGC AGCAGAAACC
 1021 GGGCAATTTT GATCTGAAAT CCGCTTTTAC TCTGAGATGA ACCCTCTGGT ATGTGGACCG
1081 ATATATATGG GACAGAAACG GAACTTAA
//
Primer: mEGFP-down2 RDB13513_A5Isb.seq
>D02675A5_A5Is_4_mEGFP-down2_E12_15.ab1
    1 CTGAATTTGC AGTCGACGAT GGCGGAGGCG AGTGAAGCCA TGTGCCTGGA GGGAGCAGAG
   61 TGGGAGCTGA GTAAAGAAAA CATACAGCCC TTACGGCACG GGCGGGTCAT GTCCACACTT
  121 CAGGGAGCTT TGGCAAAGCA AGAGTCAGCC GGCCACACTG CTCTGCAGCA GCAGAAACGG
  181 GCATTTGAAT CTGAAATCCG CTTTTACTCT GGAGATGACC CTCTGGATGT GTGGGACAGA
  241 TATATTAATT GGACAGAACA GAACTACCCT CAAGGGGGGA AAGAGAGTAA CATGTCAGCG
  301 TTAGTGGAGA GAGCGATAGA AGCACTCCAA GGAGAGACGC GCTATTATAA TGACCCCCGC
  361 TTTCTCAGTC TCTGGATCAA ATTGGGACAT TTGTGCAATG AACCTTTGGA TATGTACAGC
  421 TATTTACAAA GCCAAGGAAT TGGCGTTTCC CTTGCCCAGT TCTATATTTC ATGGGCTGAA
  481 GAATACGAAG CTAGAGAAAA TTTCAAGAAA GCGGACATAA TATTCCAGGA AGGGATTGAA
  541 CGCAAGGCTG AGCCCCTGGA CAGACTGCAG TCCCAGCACA GACAGTTCCA GTCTCGAGTG
  601 TCGCGACAAG CTTTCTTGGC CCTTGGGAAT GAAGAGGAGA GCTTTGGAGC CTTCTGAACC
  661 ACAGAGAGCT CGCTAGCTGA GCTGAAGCAA AGGAAGAAGA TGGCCAGAGC GCCATCAGCG
  721 TGTCGAGGTG CTCTGAAAGC TCCAGTCGAG CGAAGGTCTA AATGCAGTTC CCCAGCAGTA
  781 CACGGTAATC GCAGATCACC GTTTTGATGA ATGGCGAAAC CCGGCTCTAA CGATTATAGC
  841 CTTGTACCCA CCATGGATGC ACCCTTGTGC CAGGCAAGGA AAGACTCACA GTCTAGGGAA
  901 CAATGGCCGG GAACAGCCTA GAATCAGCCT GGGAGCGAAT CCAGGTCCAG TACGATGAAG
  961 GCCCCAACTG AACCTGATGC TATACCGTTC CCGACCGGGA CGACCTTGGA GTCACTCCGT
1021 GAGGAGTCGG AAACT
//
Primer: RDB13509#1 RDB13513_A5Isc.seq
>D02675A5-A5Is_4_RDB13509#1_D02_11.ab1
    1 CGGGCCTTTT GTAGTGTAAG TTGGTATTAT GTAGCTTAGA GACTCCATTC GGGTGTTCTT
   61 GAGGCTGGTT TAGTGGTAAC CAGATCAAGC TATCATTTAT CTAGAGCGGC CGCTTAGGCA
  121 GGGCTCTGGG AGCCGCCTGG CTGCTTGTCT TGCTGAGTGA GCAAAGCTTC CGGGCTGATT
  181 GTCTTCCCCA GCTTCCACAG GGCTCTGTTC AGGTGGCTAT GGAATGTGGC ATCAAAAGCC
  241 CCACCCATTT CTGCTGCCAG CTCCCCCAGA ACAGACACTG TGGACTTGTC ACTGGCATTC
  301 AGAATCCGCA CAAAGAATTT ATTCCACAAT TCACTGTCTT TTAGCTCAGA GGTGCTCTGG
  361 CTAAGTTTCC AGAGGAGTCC ATCCCAGAAG ACATGGAGGT GCTCCTTGAA CAGGAGTAAG
  421 TGCGCTAGGT CTGCTATACC CAACAGATCT ACATGGTAGG GAGAAGAACA GTTAGCCAGG
  481 ATCTTTTGTC CTTCCAGGAT CTGTGCAGTC CGAAAGCCAC TATAGGCAAA CGCATCCAGC
  541 TGCACCCTCA GGTCAACACT GTAGGAGAAG TCCATGATCC TCACAGCGTG ATCGTCCTTA
  601 TTTACATAGT CATAGGGGTC GTGGATTCTG TTTCGTAGGA TCAGACTCCG TGGACTCAAG
  661 TCTCCGTGTA CTATTTCAGC TCTGTGTAGC TTCTCCACGA TTGTCAAGAG GTTGTAAATA
  721 ATCAACACTA TTATTTCATG AGTAACAAAT TCGCTGTGTT GGAGAAGATT CTGAAGGGTG
  781 GAGCAGTTTA TATACTGGTA CCAAACAACA TGGCCATCTT GGTACTGACA GCAGCTGCAA
  841 AGCTGGTCAT AGTCCTCATT CAGACGCTCC TTCAACTTGA GGTTGATATA AAAAATCCCA
  901 CGGGATAGGC TGAGATGATG CCTTTATCAT GGGTTAGCTC TGCAGAGCTT CTTTGGTGCC
  961 ACCCCAGAAT AACCCTGTAA TCGTCACATG TTTAGTGCTC TTGCTTGATG ACGTTAATCT
 1021 CAGACCCTAA CTCAATCTCG ACCCTATTTC AAGCACAGCA TCGGTTCGGA TCATCCACAG
1081 AAAACTTTCG CAAAAGTCAA CT
//
Primer: RDB13513#1 RDB13513_A5Isd.seq
>D02675A5-A5Is_4_RDB13513#1_E02_14.ab1
    1 CCGTTACCGA GAGGGAATGT TTGCAGGAAA GAGGATCTGG ATGGGAAAGG CCGGCCGAGG
   61 CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA ATTATTGAAG
  121 AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA GCTCCCAGTA
  181 CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT GACGGGGCAG
  241 AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA AAATCCCTAC
  301 TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT GTGCTGGAAA
  361 TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG CACCTAACAT
  421 GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA ACCATGATAA
  481 AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG AAGGAGCGTC
  541 TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC CATGTTGTTT
  601 GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC GAATTTGTTA
  661 CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG AAGCTACACA
  721 GAGCTGAAAA TAGTACACGG AGACTTGAGT CCACGGAGTC TGATCCTACG AAACAGAATC
  781 CACGACCCCT ATGACTATGT AAATAAAGGA CGATCACGCT GTGAGGATCA TGGACTTCTC
  841 CTACAGTGTT GACCCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC TTTCGGACTT
  901 GCACAGATCC TGGAAAGGAC AAAAGATCCC TGGCTAACTG TTCTTCCTCC CTACCAATGT
  961 AGATCTGTTT GGGGTATAGC CAGACCCTAG CCGCAACTTA CTTCCCTGTT CCAAGGGAGC
 1021 CACCCTTCCA TGTTCTTTCC TTGGGAATGG ACCTTCCTTC TGGGAAACCT TTAGCCCAGA
 1081 AGCCAACCCT TCTGAAGCTT AAAAAGACAC AGTTGAAAAT TTGTGGGAAT TAAATTCCTT
1141 TTGTTGCCGA TATTCTGAAA TGACCAAGGT GGGAAACAAG AAGATTTTCC CA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl