Resource data sheet
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pT2-7xTcf-ONL (#RDB13490)

Wnt signal reporter of orange nano-lantern (ONL).

Clone info. Wnt signal reporter of orange nano-lantern (ONL).
Comment Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Expression was confirmed by the depositor using HEK293A cells. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI. Please obtain transposase plasmid from Dr. Kawakami lab of National Institute of Genetics.
Vector backbone pT2-7xTcf-MCS-PGK-Puro (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name Synthetic ONL Renilla reniformis luciferase cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested. (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112(14):4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Additional terms and conditions:
The Tol2 system is covered by patents owned by the National Institute of Genetics (NIG) Japan. For use of the Tol2 system except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp.
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015)。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
付加的使用条件：
Tol2 systemは、国立遺伝学研究所が特許を保有している。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に連絡すること。
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB13490 pT2-7xTcf-ONL Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pT2-7xTcf-ONL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13490).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]