RDB13487 - pCAG-GFP-mScp3 cDNA

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icon below as well as clone information before placing your order.

pCAG-GFP-mScp3 cDNA (#RDB13487)

Visualization of chromosome axes. Expression vector of mouse Scp3 in mammalian cells.

Clone info.	Expression vector of mouse Scp3 in mammalian cells. Visualization of chromosome axes.
Comment	Refer Proc Natl Acad Sci U S A. 101 (1) :16-22 (2004) as a reference.
Vector backbone	pCAGGS (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Mouse Scp3 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Watanabe, Yoshinori \| Shibuya, Hiroki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014)。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shibuya, H., Nat. Cell Biol. 16 (2): 145-156 (2014)。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB13487	pCAG-GFP-mScp3 cDNA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAG-GFP-mScp3 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13487).

Reference section:

Shibuya, H., Ishiguro, K., Watanabe, Y., The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat. Cell Biol. 16 (2): 145-156 (2014). PMID 24413433. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13487_A6Dqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB13487_A6Dqa.seq
>D02365A2_A6Dq_1_pAxCA_F1_G02_20.ab1 1 CTTAGCTTGC GTTGCTCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT 61 GCTGGTTATT GTGCTGTCTC ATCATTTTGG CAAAGAATTC GCCACCATGG TGAGCAAGGG 121 CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG 181 CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT 241 GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT 301 GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT 361 CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG 421 CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA 481 GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA 541 CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA 601 CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA 661 GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA 721 GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT 781 GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGCTCGAGA TGCTTCGAGG 841 GTGTGGGGAC AGCGACAGCT CACCGGAGCC GCTGAGCAAA CATCTAAAGA TGGTGCCTGG 901 TGGAAGAAAG CATTCTGGGA AATCTGGGAA GCCACCCTTT GGGTTGATCA GCCTAAAAAA 961 GCCCTTTGAC TTTGGAGAAG AATGATAAGA ATCTGTCTTG GATCCAGAGA GAATGGTGCT 1021 GGATGATAGG CTTCAGTAAT GTATACATGC AGAAGATCTG CTGGGAATAA //
Primer: pAxCA_R1 Sequence file: RDB13487_A6Dqb.seq
>D02365A2_A6Dq_1_pAxCA_R1_A06_03.ab1 1 TGAACAGGGG CAGTGGCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC ACCTGAGGAG 61 TGCGGCCGCT CAGAATAACA TGGATTGAAG AGACTTTCGA ACATTTGCCA TCTCTTGCTG 121 CTGAGTTTCC ATCATAACTT TTTTTTGCAA CATAGCCATT TCTTTTTTAA GTTCACTTTG 181 TGTGCCAGTA AATAGATTAT CATTATTCTT TTCCACATCT TCCAAACTCT TTATGAACTG 241 CTCGTGTATC TGTTTGATTG CTTTCATTCT CTGGCTCTGA ACAATTCTAG ACTGCTGAAA 301 AATCTTTTGT TGTTGTCGAA AAAGATTAGA TAGTTTTTCT CCTTGTTCCT CAAATTTCTG 361 TATATCCAGT TCCCACTGCT GCAACACATT CATAAATTGC TGAGAATATT CATTGTTAAG 421 CTTCTGTATT TCCTCTTGTT GTGTTTTCCA AATTTGCTCA ATTTTCTGGT TACTGGCTTT 481 GAAAGAAGCT TTGGTATACA TTTCTATTCT TTTCCTCTTG GCAAGAAGAG CTTTGTTGAT 541 GTCAGCTCCA AATTTTTCCA GCATATTCTG TACTTCACCT CCAACATCTT CAATTATCCC 601 AGCAGATCTT TTCTTTCCAT GTTTATCAAT TACTGGAGCC TTTTCATCAG CAACATCTTC 661 TTCTGAACCA GACAGATCTT TATCATCTTT CTCAAAGTCA AAGGCTTTTT TAGGCTGATC 721 AACCAAAGGT GGCTTCCCAG ATTTCCCAGA ATGCTTTCTT CCACCAGGCA CCATCTTTAG 781 ATGTTTGCTC AGCGGCTCCG GTGAGCTGTC GCTGTCCCCA CACCCTCGAA GCATCTCGAG 841 CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC 901 CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAAGGGC GGACTGGGGT GCTCAGGTAG 961 TGGTTGTTCG GGCAGCAGCA CGGGGGCCCG TTCGCCGGAT GGGGGGTGTT TCTGCTGGGT 1021 AGTGTTCGGC CGAGGCTGCA CGGCTGCCGA TCTCGATGGT TGGATGGTCG GAATTTCTTT 1081 TAAAAGGATC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shibuya, H., The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nat. Cell Biol. 16 (2): 145-156 (2014). PMID 24413433. [link to RRC of NBRP]