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pCS2P-krtt1c19e-linker-eGFP-mut-donor

Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus

Catalog number RDB13481
Resource name pCS2P-krtt1c19e-linker-eGFP-mut-donor
Clone info. Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus
CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone pCS2p (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Zebrafish krtt1c19e genomic DNA Aquorea victoria Green flurescent protein (GFP) cDNA
Depositor Kawahara, Atsuo | Hisano, Yu |
 
Other clones in our bank Zebrafish krtt1c19e | Aquorea victoria |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci. Rep., 5, 8841 (2015))
(Japanese text [open/close])
Remarks ((Additional form)) GFP-A
Please use the Exclusive MTA (genome editing) [Word] [pdf]
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13481 pCS2P-krtt1c19e-linker-eGFP-mut-donor DNA solution

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13481_A5L8p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_R3 RDB13481_A5L8a.seq
>13481_13481_A5L8_f1ori_R3_G12_21.ab1
    1 GGAATGCCCA TTCAGGCTGC GCACTGTTGG GAGGGCGATC GGTGCGGGCC TCTTCGCTAT
   61 TACGCCAGTC GAGGCGAGGG CGATGCCACC TACGGAGAGG TGATTGATGG AAAGGTGGTT
  121 TCTTCCTCTT CCACGGGAGG AGGTGGTTCA GGTGGTGGAG GATCTGGAGG TGGAGGTTCA
  181 GAATTCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
  241 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA AGGGGACGCT
  301 ACATACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
  361 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
  421 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
  481 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
  541 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
  601 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
  661 AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
  721 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
  781 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
  841 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
  901 AAGTAATCTA GATTCTGCAG CCCTATAGTG AGTCGTATTA CGTAGATCCA GACATGATAA
  961 GATACATTGA TGAGTTTGGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
 1021 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCAATA ATAAGCTGCA TAACAAGCTA
1081 ACCAACAACA ACTGGCCAAT TTCAATTTTT ATGGATTTCC AGCTTTCCAG
//
Primer: Reverse2 RDB13481_A5L8b.seq
>13481_13481_A5L8_Reverse2_H12_24.ab1
    1 ATTTCCATGA ATTACGCCAA GCGCGCAATT AACCCTCACT GGCGAGGGCG ATGCCACCTA
   61 CGGTTTACCC GACATGAATC TGGTGTGCTT TACTTAACAA GGGCACACCT CCCCCTGAAC
  121 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT
  181 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
  241 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTA CGTAATACGA
  301 CTCACTATAG GGCTGCAGAA TCTAGATTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC
  361 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
  421 CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG
  481 ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG
  541 CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG
  601 TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG
  661 ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG
  721 GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC
  781 ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC
  841 ACGCCGTAGT CAGGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG
  901 CAGATGAACT TCAGGGTCAG CTTGCCGTAT GTAGCGTCCC CTTTCGCCCT CGCCGGACAC
  961 GCTGAAACTT GTGGCCGTTT ACGTCGCCCG TCCAGCTCGA CCCAGGATGG GCACCACCCC
 1021 CGGGTGAACA GCTCCTCGCC CCTTGCTCAC CATGAATTTC TGAACCTCAC TCAGATCCTT
1081 CCACCCACCT GACCAACTCT TCCGTGAAGA GGAGAACCCA CCTTTTCCCA AA
//

Please visit plasmidSequencing and PCR primers for primer information.

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

2017.10.19

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