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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCS2P-krtt1c19e-linker-eGFP-mut-donor

Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus

Catalog number RDB13481
Resource name pCS2P-krtt1c19e-linker-eGFP-mut-donor
Clone info. Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus
Comment CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone pCS2p (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Zebrafish krtt1c19e genomic DNA Aquorea victoria Green flurescent protein (GFP) cDNA
Depositor|Developer Kawahara, Atsuo | Hisano, Yu |
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External Database
Zebrafish krtt1c19e Aquorea victoria Green flurescent protein (GFP)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci. Rep., 5, 8841 (2015))
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep., 5, 8841 (2015))
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13481 pCS2P-krtt1c19e-linker-eGFP-mut-donor DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB13481z.seq

          Original reference

          original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13481_A5L8p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: f1ori_R3
          Region: eGFP-gRNA,homology arm,linker,eGFP,SV40p(A)
          Sequence file: RDB13481_A5L8c.seq check
          >13481_13481_A5L8_f1ori_R3_G12_21.ab1
              1 GGAATGCCCA TTCAGGCTGC GCACTGTTGG GAGGGCGATC GGTGCGGGCC TCTTCGCTAT
             61 TACGCCAGTC GAGGCGAGGG CGATGCCACC TACGGAGAGG TGATTGATGG AAAGGTGGTT
            121 TCTTCCTCTT CCACGGGAGG AGGTGGTTCA GGTGGTGGAG GATCTGGAGG TGGAGGTTCA
            181 GAATTCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
            241 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA AGGGGACGCT
            301 ACATACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
            361 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
            421 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
            481 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
            541 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
            601 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
            661 AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
            721 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
            781 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
            841 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
            901 AAGTAATCTA GATTCTGCAG CCCTATAGTG AGTCGTATTA CGTAGATCCA GACATGATAA
            961 GATACATTGA TGAGTTTGGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT
           1021 TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCAATA ATAAGCTGCA TAACAAGCTA
          1081 ACCAACAACA ACTGGCCAAT TTCAATTTTT ATGGATTTCC AGCTTTCCAG
          //
          Primer: Reverse-2
          Region: eGFP-gRNA,homology arm,SV40p(A),eGFP
          Sequence file: RDB13481_A5L8d.seq check
          >13481_13481_A5L8_Reverse2_H12_24.ab1
              1 ATTTCCATGA ATTACGCCAA GCGCGCAATT AACCCTCACT GGCGAGGGCG ATGCCACCTA
             61 CGGTTTACCC GACATGAATC TGGTGTGCTT TACTTAACAA GGGCACACCT CCCCCTGAAC
            121 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT
            181 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
            241 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTA CGTAATACGA
            301 CTCACTATAG GGCTGCAGAA TCTAGATTAC TTGTACAGCT CGTCCATGCC GAGAGTGATC
            361 CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC GCTTCTCGTT GGGGTCTTTG
            421 CTCAGGGCGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA GCAGCACGGG GCCGTCGCCG
            481 ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC GATGTTGTGG
            541 CGGATCTTGA AGTTCACCTT GATGCCGTTC TTCTGCTTGT CGGCCATGAT ATAGACGTTG
            601 TGGCTGTTGT AGTTGTACTC CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG
            661 ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG
            721 GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGC
            781 ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG GGTAGCGGCT GAAGCACTGC
            841 ACGCCGTAGT CAGGGGTGGT CACGAGGGTG GGCCAGGGCA CGGGCAGCTT GCCGGTGGTG
            901 CAGATGAACT TCAGGGTCAG CTTGCCGTAT GTAGCGTCCC CTTTCGCCCT CGCCGGACAC
            961 GCTGAAACTT GTGGCCGTTT ACGTCGCCCG TCCAGCTCGA CCCAGGATGG GCACCACCCC
           1021 CGGGTGAACA GCTCCTCGCC CCTTGCTCAC CATGAATTTC TGAACCTCAC TCAGATCCTT
          1081 CCACCCACCT GACCAACTCT TCCGTGAAGA GGAGAACCCA CCTTTTCCCA AA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl