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pCS2P-krtt1c19e-linker-eGFP-mut-donor (#RDB13481)

Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hisano, Y., Sci. Rep. 5: 8841 (2015). PMID 25740433. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Donor vector for integrating gRNA-resistant eGFP mutant into zebrafish krtt1c19e locus
Comment CMV promoter of the pCS2p vector was removed by HindIII-SalI digestion.
Vector backbone pCS2p (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Growth conditions LB + Amp, 37oC
Gene/insert name Zebrafish krtt1c19e genomic DNA Aquorea victoria Green flurescent protein (GFP) cDNA
Depositor|Developer Kawahara, Atsuo | Hisano, Yu |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sci. Rep., 5, 8841 (2015))
提供案内 (日本国内) [open/close]

必要書類
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Sci Rep., 5, 8841 (2015))

Catalog # Resource name Shipping form Fee
RDB13481 pCS2P-krtt1c19e-linker-eGFP-mut-donor DNA solution

How to cite this biological resource

Materials & Methods section:

The pCS2P-krtt1c19e-linker-eGFP-mut-donor was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13481).

Reference section:

Hisano, Y., Sakuma, T., Nakade, S., Ohga, R., Ota, S., Okamoto, H., Yamamoto, T., Kawahara, A., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hisano, Y., Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish. Sci. Rep. 5: 8841 (2015). PMID 25740433. [PubMed] [Article] [RRC of NBRP]